Difference between revisions of "Team:CU-Boulder/Notebook"

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<h2> Week 3 </h2>
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<h2> Week 3: 5/22/16-5/28/16 </h2>
 
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<a name="0822"></a>
<h2> Week 4 </h2>  
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<h2> Week 4: 5/29/16-6/4/16 </h2>  
 
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<h2> Week 5 </h2>
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<h2> Week 5: 6/5/16-6/11/16 </h2>
 
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<a name="0905"></a>
<h2> Week 6 </h2>
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<h2> Week 6: 6/12/16-6/18/16 </h2>
 
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<p class = "main" > BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb </p>
 
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<a name="0912"></a>
<h2> Week 7 </h2>  
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<h2> Week 7: 6/19/16-6/25/16 </h2>  
 
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<p class = "main" > BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
 
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<a name="0919"></a>
<h2> Week 8 </h2>
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<h2> Week 8: 6/26/16-7/2/16 </h2>
 
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<p class = "main" > BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
 
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<a name="0926"></a>
<h2> Week 9 </h2>
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<h2> Week 9: 7/3/16-7/9/16 </h2>
 
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<a name="1010"></a>
<h2> Week 11</h2>
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<h2> Week 11: 7/10/16-7/16/16</h2>
 
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<p class = "main" > BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb
 
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<a name="1017"></a>
<h2> Week 12 </h2>
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<h2> Week 12: 7/17/16-7/23/16 </h2>
 
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<p class = "main" > BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb</p>
 
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<a name="1024"></a>
<h2> Week 13 </h2>  
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<h2> Week 13: 7/24/16-7/30/16 </h2>  
 
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<h2> Week 14 </h2>
+
<h2> Week 14: 7/31/16-8/6/16 </h2>
 
<figure style="float:right">
 
<figure style="float:right">
 
     <img src="https://static.igem.org/mediawiki/2016/e/e3/Jonah1.jpg" style="width:350px;height:458px;margin-right: 70px; padding: 10px 10px 10px 10px; float:right">
 
     <img src="https://static.igem.org/mediawiki/2016/e/e3/Jonah1.jpg" style="width:350px;height:458px;margin-right: 70px; padding: 10px 10px 10px 10px; float:right">
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<h2> Week 15 </h2>
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<h2> Week 15: 8/7/16-8/13/16 </h2>
 
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<h2> Week 17 </h2>
+
<h2> Week 17: 8/14/16-8/20/16 </h2>
 
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Revision as of 18:00, 9 August 2016

May
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June
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July
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October
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Notebook

Week 1: 5/8/16-5/14/16

Fig.1 - Jonah and his creepy smile

Successful microscopy has confirmed the viability of EutS and EutC-eGFP in E.Coli, but the laser used to excite eGFP may also cause conformational change of azo-benzene. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.

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Week 2: 5/15/16-5/21/16

Fig.2 - Lane 2: eGFP-Hi 1c3 Lane 3: eGFP-Hi 1c3 Lane 4: ladder Lane 5: eGFP-Lac 1c3 Lane 6: eGFP-Lac 1c3

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 3: 5/22/16-5/28/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 4: 5/29/16-6/4/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 5: 6/5/16-6/11/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 6: 6/12/16-6/18/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 7: 6/19/16-6/25/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 8: 6/26/16-7/2/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 9: 7/3/16-7/9/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 10

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 11: 7/10/16-7/16/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 12: 7/17/16-7/23/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 13: 7/24/16-7/30/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 14: 7/31/16-8/6/16

Fig.1 - Jonah and his creepy smile

Successful microscopy has confirmed the viability of EutS and EutC-eGFP in E.Coli, but the laser used to excite eGFP may also cause conformational change of azo-benzene. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.

Go to top

Week 15: 8/7/16-8/13/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 17: 8/14/16-8/20/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 18

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 19

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 20

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 21

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 22

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 23

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 24

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 25

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 26

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 27

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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