Difference between revisions of "Team:CU-Boulder/Notebook"

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<figcaption style = "padding: 10px 10px 10px;">Fig.2 - Lane 2: eGFP-Hi 1c3 Lane 3: eGFP-Hi 1c3 Lane 4: ladder Lane 5: eGFP-Lac 1c3 Lane 6: eGFP-Lac 1c3</figcaption>
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<figcaption style = "padding: 10px 10px 10px;"> Gel.1 - Lane 2: eGFP-Hi 1c3 Lane 3: eGFP-Hi 1c3 Lane 4: ladder Lane 5: eGFP-Lac 1c3 Lane 6: eGFP-Lac 1c3</figcaption>
 
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<h2> Week 14: 7/31/16-8/6/16 </h2>
 
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<figcaption style = "padding: 10px 10px 10px;">Gel.5 - Lane 1: Ladder Lane 2: EutS-Hi Lane 3: EutS-Hi Lane 4: EutS-Hi Lane 5: Ladder Lane 6: EutS-Lac 6A1 Lane 7: EutS-Lac 4A5 Lane 8: EutS-Lac 4A5</figcaption>
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Revision as of 19:03, 9 August 2016

May
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June
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August
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September
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October
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Notebook

Week 1: 5/8/16-5/14/16

Fig.1 - Jonah and his creepy smile

Successful microscopy has confirmed the viability of EutS and EutC-eGFP in E.Coli, but the laser used to excite eGFP may also cause conformational change of azo-benzene. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.

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Week 2: 5/15/16-5/21/16

Gel.1 - Lane 2: eGFP-Hi 1c3 Lane 3: eGFP-Hi 1c3 Lane 4: ladder Lane 5: eGFP-Lac 1c3 Lane 6: eGFP-Lac 1c3

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 3: 5/22/16-5/28/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 4: 5/29/16-6/4/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 5: 6/5/16-6/11/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 6: 6/12/16-6/18/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 7: 6/19/16-6/25/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 8: 6/26/16-7/2/16

Gel.2 - Lane 2: bad cut with E Lane 3: Nep-Lo 1k3 Lane 4: Nep-Lo 1k3 Lane 5: Ladder Lane 6: eGFP-Hi 1k3 Lane 7: eGFP-Hi 1k3

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 9: 7/3/16-7/9/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 10

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 11: 7/10/16-7/16/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 12: 7/17/16-7/23/16

Gel.3 - Lane 2: eGFP-Hi 4A5 Lane 3: eGFP-Hi 4A5 Lane 4: Ladder Lane 5: eGFP-Hi 4A5 Lane 6: eGFP-Hi 4A5. Lane 3 and Lane 5 look good

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 13: 7/24/16-7/30/16

Gel.4 - Lane 1: Ladder Lane 2: EutS- Hi 6A1 Lane 3: EutS-Hi 4A5 Lane 4: EutS-Hi 4A5 Lane 5: Ladder Lane 6: eGFP-Hi Lane 7: eGFP-Hi. eGFP is good

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 14: 7/31/16-8/6/16

Gel.5 - Lane 1: Ladder Lane 2: EutS-Hi Lane 3: EutS-Hi Lane 4: EutS-Hi Lane 5: Ladder Lane 6: EutS-Lac 6A1 Lane 7: EutS-Lac 4A5 Lane 8: EutS-Lac 4A5

Successful microscopy has confirmed the viability of EutS and EutC-eGFP in E.Coli, but the laser used to excite eGFP may also cause conformational change of azo-benzene. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.Instead Neptune, another light activated protein that is excited at a much longer wavelength, will be used to visualize the formation and destruction of EutS microcompartments. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance. Future work will focus on the implementation of a multiconstruct system with EutS, EuC-Neptune, and a third construct that creates tRNA’s to integrate azo-benzene into the EutS at locations we expect to see significant steric hinderance.

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Week 15: 8/7/16-8/13/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 17: 8/14/16-8/20/16

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 18

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 19

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 20

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 21

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 22

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 23

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 24

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 25

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 26

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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Week 27

BBBBBBBBBBBBB BBBBB BBBBBBBBBBBBBBB BBBBBBBBBBBBBBBBB BBBBBBB BBBBB BBBBBBBB BBBBB bbbbbb bbb bbbbb bb bb bbbbbbbbbbb bb bbbbbbbb

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