(→pPS16_010 extraction) |
(→pPS16_010 extraction) |
||
Line 12: | Line 12: | ||
====[[Team:Paris_Saclay/Experiments#pPS16_010|pPS16_010]] extraction==== | ====[[Team:Paris_Saclay/Experiments#pPS16_010|pPS16_010]] extraction==== | ||
''By Alice'' | ''By Alice'' | ||
+ | |||
After screening colony screening PCR, 3 clones (clones 3, 7 and 8) transformed with pPS16_010 seemed to have the insert of interest. Plasmids from these clones were extracted following [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|this protocol]]. After extraction, plasmids were migrated on a gel.z | After screening colony screening PCR, 3 clones (clones 3, 7 and 8) transformed with pPS16_010 seemed to have the insert of interest. Plasmids from these clones were extracted following [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|this protocol]]. After extraction, plasmids were migrated on a gel.z | ||
[[File:T--Paris_Saclay--20160811_extraction_FRB_3_7_8.jpeg|400px|thumb|right|Migration of FRB plasmid]] | [[File:T--Paris_Saclay--20160811_extraction_FRB_3_7_8.jpeg|400px|thumb|right|Migration of FRB plasmid]] | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} |
Revision as of 15:06, 11 August 2016