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<h1><center>Alverno iGEM Interlab Study</center></h1> | <h1><center>Alverno iGEM Interlab Study</center></h1> | ||
+ | <h1><center>Quantifying different sources of variation in gene expression using | ||
+ | fluorescent transformed E. coli bacteria</center></h1> | ||
<center><img src="https://s-media-cache-ak0.pinimg.com/originals/86/08/18/860818cfc65e5ff04725bb0f0c05a8af.png" alt="Alverno iGEM Logo" style="width:300px;"></center> | <center><img src="https://s-media-cache-ak0.pinimg.com/originals/86/08/18/860818cfc65e5ff04725bb0f0c05a8af.png" alt="Alverno iGEM Logo" style="width:300px;"></center> | ||
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<p><h3>Project Description:</h3></p> | <p><h3>Project Description:</h3></p> | ||
− | <p> | + | <p>We tested 3 different fluorescent protein expression plasmids, J23101, J23106, and J23117. We transformed the parts with iGEM's transformation protocol. Our bacteria was cultured on a LB agar plate and cultured in LB broth. To measure the differences in fluorescence caused by each plasmid, we used a plate reader with a OD600 reference point and FITC standard curve.<p> |
− | + | ||
<p><h3>Protocol:</h3> </p> | <p><h3>Protocol:</h3> </p> |
Revision as of 17:49, 12 August 2016
Alverno iGEM Interlab Study
Quantifying different sources of variation in gene expression using
fluorescent transformed E. coli bacteria
Summary:
Leverage agile frameworks to provide a robust synopsis for high level overviews. Iterative approaches to corporate strategy foster collaborative thinking to further the overall value proposition. Organically grow the holistic world view of disruptive innovation via workplace diversity and empowerment.
Project Description:
We tested 3 different fluorescent protein expression plasmids, J23101, J23106, and J23117. We transformed the parts with iGEM's transformation protocol. Our bacteria was cultured on a LB agar plate and cultured in LB broth. To measure the differences in fluorescence caused by each plasmid, we used a plate reader with a OD600 reference point and FITC standard curve.
Protocol:
Podcasting operational change management inside of workflows to establish a framework. Taking seamless key performance indicators offline to maximise the long tail. Keeping your eye on the ball while performing a deep dive on the start-up mentality to derive convergence on cross-platform integration.