Difference between revisions of "Team:DTU-Denmark"

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<h2 style="color:black"> Our Team </h2>
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<p>We are DTU BioBuilders, the iGEM team of the Technical University of Denmark. We study biotechnolgy, bioinformatics, mathematical modelling, environmental engineering and biomedical engineering. Our team consists of 13 graduates and 3 undergraduates. In addition, we have 3 highschool students assisting us in the lab. Our team members come from Denmark, Germany, the Netherlands, Greece, China and Poland. This year we tackle substrate utilization in cell factories and this is our wiki:</p>
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<h2>The brainstorm</h2>
 
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<p>We tried to identify a project that meets the following criteria:
 
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<ul>
 
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<li>Identify a local challenge that has a global impact. </li>
 
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<li>To tackle iGEM with a "Cell Factory Engineering"- perspective</li>
 
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<li>Make a novel contribution to the biobrick registry </li>
 
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<h2> The problem: Waste </h2>
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<p>In Denmark today, less than half the waste produced is recycled, which means that more than 3.5 million tons get burned off each year.</p>
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          <h2>The <strong>problem</strong></h2>
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<p>We have abundant waste streams from the industry such as glycerol from biodiesel production, byproducts from rapeseed production, used cooking oil and ordinary household waste.</p>
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<p><strong>
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The current state of industrial biotechnology means that the vast majority biorefineries relies on edible substrates such as corn, wheat or sugar canes. This has sparked sparked the food vs. fuel debate, leading to the fundamental question: “Should we use our edible crops to feed the growing human population, or use it to provide sustainable chemicals to the industrialised world?”. A better question might be: “Why are we not doing both?”. The limiting factor of current processes, is a lack of molecular tools that has limited us to rely on a small number of organisms with narrow substrate ranges. Even though efforts has been made to expand the substrate range of many conventional cell factories, such as Escherichia coli and Saccharomyces cerevisiae, the task has proven difficult and real impact has soon to come as a result of these experiments
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<p>Cell factories are becoming an increasing factor in the industry today, where different microorganisms are utilized to produce various compounds from therapeutics to food additives. Currently however, the sustainability of these industrial processes is limited by the narrow substrate range of the organisms used. The most common feeds in use are simple carbohydrates such as glucose produced by enzymatic hydrolysis from edible plants such as maize, rice and wheat.</p>
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        <h2>The <strong>solution</strong></h2>
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        The development of new technologies such as CRISPR and Next-generation sequencing has dramatically reduced the effort required to genetically modify non-model organisms, and is effectively breaking down the barrier between model and non-model organism. Therefore we though: “Why force a model organisms to grow on non-conventional substrates, when we can start of with an organism that already grows on a broad range of substrates?”. See our proposed solution by scrolling down.
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<h2>Aim of our project </h2>
 
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<p>This project aims to develop the chassis for a versatile and efficient cell factory that
 
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can transform abundant waste streams into valuable products using <em>Yarrowia lipolytica</em> and
 
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state of the art genetic editing techniques.</p>
 
 
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<h1>This is heading 1</h1>
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<h2> Our contribution: a molecular toolbox </h2>
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<p>The reason why <em>Y. lipolytica</em> has not been implemented in industry yet is the current lack of tools for genetic engineering.</p>
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<p> In our project, we develop and test tools for Y. lipolytica. This set will standardized and based on CRISPR/Cas9-mediated genome editing. Y. lipolytica naturally comes with a high potential for biotechnological applications. By using our toolbox, anyone will be able to easily customize the genome to their needs.</p>
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<h2> Wiki template information </h2>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h2> Editing your wiki </h2>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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<h1>Explore our project</h1>
  
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Revision as of 08:13, 17 August 2016

New HTML template for the wiki




The problem

The current state of industrial biotechnology means that the vast majority biorefineries relies on edible substrates such as corn, wheat or sugar canes. This has sparked sparked the food vs. fuel debate, leading to the fundamental question: “Should we use our edible crops to feed the growing human population, or use it to provide sustainable chemicals to the industrialised world?”. A better question might be: “Why are we not doing both?”. The limiting factor of current processes, is a lack of molecular tools that has limited us to rely on a small number of organisms with narrow substrate ranges. Even though efforts has been made to expand the substrate range of many conventional cell factories, such as Escherichia coli and Saccharomyces cerevisiae, the task has proven difficult and real impact has soon to come as a result of these experiments

The solution

The development of new technologies such as CRISPR and Next-generation sequencing has dramatically reduced the effort required to genetically modify non-model organisms, and is effectively breaking down the barrier between model and non-model organism. Therefore we though: “Why force a model organisms to grow on non-conventional substrates, when we can start of with an organism that already grows on a broad range of substrates?”. See our proposed solution by scrolling down.

This is heading 1

Lorem ipsum dolor sit amet, consectetur adipiscing elit. Sed eget ornare orci. Etiam sodales molestie felis quis condimentum. Aliquam vel sem fringilla, egestas justo sit amet, tincidunt augue. Nam maximus arcu sit amet eros rutrum commodo. Quisque fermentum dapibus tristique. Fusce eu neque euismod, commodo arcu maximus, rutrum magna. Proin eget cursus dui, eu bibendum lacus. Sed ac accumsan mi. Mauris at pulvinar turpis. Nulla bibendum nec felis ut fringilla. Morbi ac faucibus ex. Ut eleifend libero non rhoncus tristique. Mauris vel ornare massa. In congue, tellus semper condimentum porta, turpis tortor porta est, ac blandit elit velit vitae ex. Cras in lectus ligula. Praesent tincidunt dui et libero commodo, vitae cursus elit finibus.

Maecenas porta vitae turpis vel lacinia. Phasellus nisl urna, auctor at placerat ac, faucibus sed ligula. Phasellus arcu nunc, elementum eget pellentesque eget, dignissim vel erat. Nullam augue urna, viverra facilisis aliquet eu, dapibus id ex. Vivamus at ipsum congue, imperdiet libero et, pretium velit. Cras vel finibus erat, ac aliquet felis. Sed congue tellus vel justo cursus, id eleifend massa scelerisque. Donec pharetra ipsum sed neque aliquet, non accumsan velit pharetra. Quisque egestas condimentum tellus id blandit. Praesent in sollicitudin turpis. Proin efficitur ut est vitae sodales. Fusce tristique, diam at pulvinar feugiat, magna dolor fringilla arcu, vel semper massa elit vel nunc. Vivamus dapibus congue odio ut varius. Nullam nunc neque, scelerisque at turpis faucibus, elementum hendrerit mi. Fusce tristique dapibus lectus quis dapibus.

Cras eget faucibus odio. Aenean pharetra elit ut metus tristique, eget blandit lorem tincidunt. Integer quis justo ac dolor accumsan lobortis vitae sit amet enim. Lorem ipsum dolor sit amet, consectetur adipiscing elit. Curabitur eros lectus, feugiat sodales fermentum quis, mattis congue velit. Aenean tempor dolor vitae nisl dignissim, sit amet aliquet neque malesuada. Nullam in neque eu tortor egestas tincidunt. Fusce suscipit odio posuere nisl hendrerit volutpat. Curabitur ornare mauris ac magna viverra hendrerit. Nulla facilisi. Quisque venenatis vitae elit consequat posuere. Cras non turpis maximus, volutpat massa dignissim, fermentum augue. Sed venenatis diam eu lacus cursus pulvinar. Maecenas urna turpis, venenatis eget nunc fringilla, dignissim luctus purus. Sed faucibus arcu a lacus condimentum blandit.

Explore our project