Difference between revisions of "Team:Paris Saclay/Notebook/August/17"

(Purification of gBlocks 2, 3 and 4)
(Purification of gBlocks 2, 3 and 4)
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''By Terrence''
 
''By Terrence''
  
The purification were carried out following the usual [[Team:Paris_Saclay/Experiments
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The purification were carried out following the usual [[Team:Paris_Saclay/Experiments#DNA_extraction_from_agarose_gels_:_with_NucleoSpin_Gel_and_PCR_Clean-up|protocol]].
#DNA_extraction_from_agarose_gels_:_with_NucleoSpin_Gel_and_PCR_Clean-up|protocol]].
+
  
 
===Visualization===
 
===Visualization===

Revision as of 12:35, 17 August 2016

Wednesday 17th August

Lab work

Visualization

Extraction of pPS16003, pPS16004 and GFP1-9_PSB1C3 (clones 2 and 3)

By Charlène

Clones 2 and 3 of GFP1-9_PSB1C3, and pPS16003, pPS16004 were extracted with the Plasmid MiniPrep kit.

The extracts were put to migrated on a 0.8%agarose gel with BET.

Purification of gBlocks 2, 3 and 4

By Terrence

The purification were carried out following the usual protocol.

Visualization

Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM

By Léa and Naiane