Difference between revisions of "Team:Paris Saclay/Notebook/August/17"

(Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM)
(Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM)
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''By Léa and Naiane''
 
''By Léa and Naiane''
  
The cloning was made with this new [[Team:Paris_Saclay/Experiments#ligation with pJet|protocol]] which uses pJET as cloning vector.
+
The cloning was carried out using a new [[Team:Paris_Saclay/Experiments#ligation with pJet|protocol]] which uses pJET as cloning vector.
A heat shock transformation was carried out with the cloning samples using the following [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]
+
A heat shock transformation was made on the cloning samples using the following [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]]
  
  

Revision as of 13:56, 17 August 2016

Wednesday 17th August

Lab work

Visualization

Extraction of pPS16003, pPS16004 and GFP1-9_PSB1C3 (clones 2 and 3)

By Charlène

Clones 2 and 3 of GFP1-9_PSB1C3, and pPS16003, pPS16004 were extracted with the Plasmid MiniPrep kit.

The extracts were put to migrated on a 0.8%agarose gel with BET.

Purification of gBlocks 2, 3 and 4

By Terrence

The purification were carried out following the usual protocol.

Visualization

Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM

By Léa and Naiane

The cloning was carried out using a new protocol which uses pJET as cloning vector. A heat shock transformation was made on the cloning samples using the following protocol