Difference between revisions of "Team:Paris Saclay/Notebook/August/17"

(Purification of gBlocks 2, 3 and 4)
(Wednesday 17th August)
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The purification was carried out following the usual [[Team:Paris_Saclay/Experiments#DNA_extraction_from_agarose_gels_with_NucleoSpin_Gel_and_PCR_Clean-up|protocol]].
 
The purification was carried out following the usual [[Team:Paris_Saclay/Experiments#DNA_extraction_from_agarose_gels_with_NucleoSpin_Gel_and_PCR_Clean-up|protocol]].
  
===Visualization===
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==== Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM ====
 
==== Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM ====
 
''By Léa and Naiane''
 
''By Léa and Naiane''
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{{Team:Paris_Saclay/notebook_footer}}
 
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 15:09, 17 August 2016

Wednesday 17th August

Lab work

Visualization

Extraction of pPS16003, pPS16004 and GFP1-9_PSB1C3 (clones 2 and 3)

By Charlène

Clones 2 and 3 of GFP1-9_PSB1C3, and pPS16003, pPS16004 were extracted with the Plasmid MiniPrep kit.

The extracts were put to migrated on a 0.8%agarose gel with BET.

Purification of gBlocks 2, 3 and 4

By Terrence

The purification was carried out following the usual protocol.


Cloning with pJET the gBlock 1.2, FRB, FKBP and SgNM

By Léa and Naiane

The cloning was carried out using a new protocol which uses pJET as cloning vector.

A heat shock transformation was made on the cloning samples using the following protocol


=