Difference between revisions of "Tracks/Measurement/Interlab study/Flow Cytometry Overview"
| Line 5: | Line 5: | ||
</div> | </div> | ||
<br><br><br><br><br> | <br><br><br><br><br> | ||
| + | <div class="oneColumn"> | ||
<h3><a href="http://parts.igem.org/Help:InterLab_Measurement_Kit">Go to InterLab Measurement Kit Information Page</a></h3> | <h3><a href="http://parts.igem.org/Help:InterLab_Measurement_Kit">Go to InterLab Measurement Kit Information Page</a></h3> | ||
| + | |||
| + | </div> | ||
<div class="oneColumn"> | <div class="oneColumn"> | ||
Revision as of 19:31, 3 June 2016
Measurement with Flow Cytometry
This is a general guide for setting up your cells for flow cytometry readings in absolute units. These steps and questions are meant to provide a general protocol and we ask that you follow them to the best of your ability.
In order to carry out this measurement protocol, you will need:
- A flow cytometer with a channel configured for measurement of fluorescein (FITC) or GFP. This channel will typically have a 488nm laser an a 530/30 filter, but the details may vary.
- Fluorescent calibration beads that have been calibrated for Molecules of Equivalent FLuorescein (MEFL) or Molecules of Equivalent GFP (MEGFP). If available, we recommend using SpheroTech Rainbow Calibration Particles RCP-30-5A, as that is what the accompanying spreadsheet has been designed for.
All teams using a flow cytometer to measure GFP must finish the following items to fulfill the InterLab:
- Email your completed Excel file to measurement (AT) igem (DOT) org [note: the empty template file is provided below]
- Fill in the Flow Cytometer Form provided at the bottom of this page
- Create an InterLab study page on your team wiki (please use this format: https://2016.igem.org/Team:YourTeamName/InterLab)
[[Excel File Template]]
Important! If you have used a flow cytometer to measure GFP for the InterLab, please fill out the following Google Form to the best of your ability:
