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<h2 class="red">Week 5th September-11th September</h2> | <h2 class="red">Week 5th September-11th September</h2> | ||
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− | + | An automated 96 well microplate for fabric was made using a fluorescent microplate, 96 well tip holder and laser-cut cotton circles. | |
− | + | 1. Spread flat a wet cotton sheet on glass substrate of 96 well plate dimension. | |
+ | <br> | ||
+ | 2. 96 Wet cotton circles of 6.5mm are laser cut on glass substrate. | ||
+ | <br> | ||
+ | 3. Remove the fabric. The circles of cotton stay on the glass substrate. | ||
+ | <br> | ||
+ | 4. Press the glass substrate against fluorescent microplate such that all circles align with the wells and move the glass substrate laterally so that all the cotton circles fall into the wells. | ||
+ | <br> | ||
+ | 5. Put the tip plate holder on the top of the microplate and adhere it to the microplate using a glue. | ||
+ | <br> | ||
+ | 6. Cover it with a lid and the microplate is ready to use on cotton fabric. The same can be done for other fabrics like silk, Polystyrene, nylon etc. | ||
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<img class="assay" src="https://static.igem.org/mediawiki/2016/b/b4/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview.jpg" alt=« Success" height=“150px“ /> | <img class="assay" src="https://static.igem.org/mediawiki/2016/b/b4/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview.jpg" alt=« Success" height=“150px“ /> | ||
<img class="assay" src="https://static.igem.org/mediawiki/2016/1/1e/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview2.jpg" alt=« Success" height=“150px“ /> | <img class="assay" src="https://static.igem.org/mediawiki/2016/1/1e/Paris_Bettencourt-Notebook_Assay_Finaldesign_topview2.jpg" alt=« Success" height=“150px“ /> | ||
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<img class="assay" src="https://static.igem.org/mediawiki/2016/7/75/Paris_Bettencourt-Notebook_Assay_Finaldesign_Dilutiontest.jpg" alt=« Success" height=“150px“ /> | <img class="assay" src="https://static.igem.org/mediawiki/2016/7/75/Paris_Bettencourt-Notebook_Assay_Finaldesign_Dilutiontest.jpg" alt=« Success" height=“150px“ /> | ||
<img class="assay" src="https://static.igem.org/mediawiki/2016/d/d6/Paris_Bettencourt-Notebook_Assay_Finaldesign_beforedilution.jpg" alt=« Success" height=“150px“ /> | <img class="assay" src="https://static.igem.org/mediawiki/2016/d/d6/Paris_Bettencourt-Notebook_Assay_Finaldesign_beforedilution.jpg" alt=« Success" height=“150px“ /> |
Revision as of 18:46, 30 September 2016
Week 5th September-11th September
An automated 96 well microplate for fabric was made using a fluorescent microplate, 96 well tip holder and laser-cut cotton circles.
1. Spread flat a wet cotton sheet on glass substrate of 96 well plate dimension.
2. 96 Wet cotton circles of 6.5mm are laser cut on glass substrate.
3. Remove the fabric. The circles of cotton stay on the glass substrate.
4. Press the glass substrate against fluorescent microplate such that all circles align with the wells and move the glass substrate laterally so that all the cotton circles fall into the wells.
5. Put the tip plate holder on the top of the microplate and adhere it to the microplate using a glue.
6. Cover it with a lid and the microplate is ready to use on cotton fabric. The same can be done for other fabrics like silk, Polystyrene, nylon etc.