Difference between revisions of "Team:Tuebingen/Measurement"

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<h1>InterLab Study</h1>
            Here is a small demonstration for a block text.
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<p>Fluorescence measurement is a method abundant in all major labs.</p>
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</div>
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<p>Following up on the past years’ success, the iGEM HQ again challenged teams to take part in the InterLab study.  
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</p>
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<p>
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Measuring the fluorescence of various gene constructs becomes more and more important during everyday lab work. However, the comparability in different labs is still under debate. Using the fact that teams all over the world participate in iGEM, this offers the perfect opportunity to see how results of the same experiment performed all over the world compare.
        sadipscing elitr, sed diam nonumy eirmod tempor invidunt ut labore et dolore
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This year’s aim was to compare the fluorescence yield of five different constructs containing GFP under control of different RBS and promoters. By using a FITC standard curve and LUDOX to correct for errors in OD600 measurements, the results of all teams can be easily compared.  
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</p>
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        <figure>
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<p> Materials and Methods</p>
            <img src='../wiki/images/9/9b/Harry.jpg'' />
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</div>
            <figcaption>
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<div class="contentCell">
                Take a look at this awesome illustration!
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<p>The following five constructs from the InterLab kit were used to transform E. coli DH10beta:
            </figcaption>
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</p>
        </figure>
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<li>Positive Control</li>
        <p>
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<li>Negative control</li>
            And it still keeps going!
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<li>Device 1: J23101+I13504</li>
        </p>
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<li>Device 2: J23106+I13504</li>
    </div>
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<li>Device 3: J23117+I13504</li>
 
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<p>
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FITC and LUDOX standards were measured in a TECAN plate reader, using 96-well plates with transparent bottoms. LUDOX was measured at 600nm with a bandwidth of 9 nm and 25 flashes. FITC was excited at 488 nm (bandwidth 9 nm) and emission was recorded at 520 nm (bandwidth 20nm) with a fixed gain of 50 and 25 flashes. Identical settings were used for measurement of the devices.
        <div class='contentCell contentSmallCell'>
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</p>
            <figure>
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<p>All measurements were done as described in the Interlab plate reader protocol (see <a href="https://2016.igem.org/Team:Tuebingen/Methods">Methods</a>). Transformations were performed as described <a href=#>here</a>.
                <img src='../wiki/images/9/9b/Harry.jpg' />
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                <figcaption>
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</div>
                    -- Some wizzard dude.
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                </figcaption>
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<figure>
            </figure>
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<img src="https://static.igem.org/mediawiki/2016/1/11/T--Tuebingen--fig1_fitc_standarg_interlab.png" style="cursor: zoom-in;">  
            <p>
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<figcaption>shows the FITC standard curve used for the following calculations.</figcaption>  
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                justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea
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                takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum
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                dolor sit amet, consetetur sadipscing elitr, sed diam nonumy
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                eirmod tempor invidunt ut labore et dolore magna aliquyam erat,
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                sed diam voluptua. At vero eos et accusam et justo duo dolores
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                et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus
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                consetetur sadipscing elitr, sed diam nonumy eirmod tempor
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                invidunt ut labore et dolore magna aliquyam erat, sed diam
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                voluptua. At vero eos et accusam et justo duo dolores et ea
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                rebum. Stet clita kasd gubergren, no sea takimata sanctus est
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                Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet,
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                consetetur sadipscing elitr, sed diam nonumy eirmod tempor...
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                aliquyam erat, sed diam voluptua. At vero eos et accusam et
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                justo duo dolores et ea rebum. Stet clita kasd gubergren, no sea
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                takimata sanctus est Lorem ipsum dolor sit amet. Lorem ipsum
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                dolor sit amet, consetetur sadipscing elitr, sed diam nonumy
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                eirmod tempor invidunt ut labore et dolore magna aliquyam erat,
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                sed diam voluptua. At vero eos et accusam et justo duo dolores
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                et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus
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                est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet,
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                consetetur sadipscing elitr, sed diam nonumy eirmod tempor
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                invidunt ut labore et dolore magna aliquyam erat, sed diam
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                voluptua. At vero eos et accusam et justo duo dolores et ea
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                rebum. Stet clita kasd gubergren, no sea takimata sanctus est
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                Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet,
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                consetetur sadipscing elitr, sed diam nonumy eirmod tempor...
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                Here is a small demonstration for a block text.  Lorem ipsum
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                eirmod tempor invidunt ut labore et dolore magna aliquyam erat,
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                sed diam voluptua. At vero eos et accusam et justo duo dolores
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                et ea rebum. Stet clita kasd gubergren, no sea takimata sanctus
+
                est Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet,
+
                consetetur sadipscing elitr, sed diam nonumy eirmod tempor
+
                invidunt ut labore et dolore magna aliquyam erat, sed diam
+
                voluptua. At vero eos et accusam et justo duo dolores et ea
+
                rebum. Stet clita kasd gubergren, no sea takimata sanctus est
+
                Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet,
+
                consetetur sadipscing elitr, sed diam nonumy eirmod tempor
+
                invidunt ut labore et dolore magna aliquyam erat, sed diam
+
                voluptua. At vero eos et accusam et justo duo dolores et ea
+
                rebum. Stet clita kasd gubergren, no sea takimata sanctus est
+
                Lorem ipsum dolor sit amet. Lorem ipsum dolor sit amet,
+
                consetetur sadipscing elitr, sed diam nonumy eirmod tempor...
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            </p>
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            <table>
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                <caption>Uebersicht Saeuremienenarbeit:</caption>
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                <thead>
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                    <tr>
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                        <td>Year</td>
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                        <td>Slaves</td>
+
                        <td>Income</td>
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                        <td>Bribes</td>
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                    </tr>
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                </thead>
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                <tbody>
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                    <tr>
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                        <td>2009</td>
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                        <td>9</td>
+
                        <td>$1 mio.</td>
+
                        <td>0</td>
+
                    </tr>
+
                    <tr>
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                        <td>2010</td>
+
                        <td>90</td>
+
                        <td>-$1 mio.</td>
+
                        <td>$20,000</td>
+
                    </tr>
+
                    <tr>
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                        <td>2011</td>
+
                        <td>100</td>
+
                        <td>$10 mio.</td>
+
                        <td>$50,000</td>
+
                    </tr>
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                </tbody>
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            </table>
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            <p>
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                Don't forget to refer to tables in a text. Need
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                feature btw: Auto counters!
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            <p>
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                aliquyam erat, sed diam voluptua. At vero eos et accusam et
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                justo duo dolores et ea rebum. Stet clita kasd gubergren,
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            </p>
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            <table>
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                <caption>Uebersicht Saeuremienenarbeit:</caption>
+
                <thead>
+
                    <tr>
+
                        <td>Year</td>
+
                        <td>Slaves</td>
+
                        <td>Income</td>
+
                        <td>Bribes</td>
+
                    </tr>
+
                </thead>
+
                <tbody>
+
                    <tr>
+
                        <td>2009</td>
+
                        <td>9</td>
+
                        <td>$1 mio.</td>
+
                        <td>0</td>
+
                    </tr>
+
                    <tr>
+
                        <td>2010</td>
+
                        <td>90</td>
+
                        <td>-$1 mio.</td>
+
                        <td>$20,000</td>
+
                    </tr>
+
                    <tr>
+
                        <td>2011</td>
+
                        <td>100</td>
+
                        <td>$10 mio.</td>
+
                        <td>$50,000</td>
+
                    </tr>
+
                </tbody>
+
            </table>
+
        </div>
+
    </div>
+
 
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Revision as of 15:01, 18 October 2016

InterLab Study

Fluorescence measurement is a method abundant in all major labs.

Following up on the past years’ success, the iGEM HQ again challenged teams to take part in the InterLab study.

Measuring the fluorescence of various gene constructs becomes more and more important during everyday lab work. However, the comparability in different labs is still under debate. Using the fact that teams all over the world participate in iGEM, this offers the perfect opportunity to see how results of the same experiment performed all over the world compare. This year’s aim was to compare the fluorescence yield of five different constructs containing GFP under control of different RBS and promoters. By using a FITC standard curve and LUDOX to correct for errors in OD600 measurements, the results of all teams can be easily compared.

Materials and Methods

The following five constructs from the InterLab kit were used to transform E. coli DH10beta:

  • Positive Control
  • Negative control
  • Device 1: J23101+I13504
  • Device 2: J23106+I13504
  • Device 3: J23117+I13504
  • FITC and LUDOX standards were measured in a TECAN plate reader, using 96-well plates with transparent bottoms. LUDOX was measured at 600nm with a bandwidth of 9 nm and 25 flashes. FITC was excited at 488 nm (bandwidth 9 nm) and emission was recorded at 520 nm (bandwidth 20nm) with a fixed gain of 50 and 25 flashes. Identical settings were used for measurement of the devices.

    All measurements were done as described in the Interlab plate reader protocol (see Methods). Transformations were performed as described here.

    shows the FITC standard curve used for the following calculations.