Revision as of 00:12, 6 October 2016

Making the Agarose Gel

Experiment & Protocol

*Note: Pipettes are needed.

Ingredients/Materials: *

- 0.6g agarose

- 50mL TBE 1x

- 5μL SYBRsafe / 2.5μL EcoStain (light- and heat-sensitive)

- 250mL conical flask

- scale

- Microwave

- Gel mold

Directions:

1. Weigh 0.6g of agarose into flask.

2. Add 50mL of TBE 1x Buffer

3. Microwave agarose solution until dissolved for 1 min (take out halfway to swirl)

(If the liquid looks distorted, agarose solution need to be microwaved more)

4. Cool slightly and add SYBRsafe or EcoStain

5. Pour gel into mold and put the combs into their spots

6. Cool until solidified.

@@ Line 10: / Line 10: @@
 <h1><center>Experiment & Protocol</center></h1>
 <center><img src="https://s-media-cache-ak0.pinimg.com/originals/86/08/18/860818cfc65e5ff04725bb0f0c05a8af.png" alt="Alverno iGEM Logo" style="width:300px;"></center>
-<h1><center>Making the Agarose Gel</center></h1>
+<h1><center>Making the Agarose Gel</center><img src="https://static.igem.org/mediawiki/2016/5/50/T--Alverno_CA--ProtocolPicture_Gel.png" alt="A Picture of a Gel  style="width:100px;"></h1>
 <p>       *Note: Pipettes are needed. </p>
 <body>
 <center><p>Ingredients/Materials: *</p></center>
-<p>- 0.6g agarose <img src="https://static.igem.org/mediawiki/2016/5/50/T--Alverno_CA--ProtocolPicture_Gel.png" alt="A Picture of a Gel  style="width:200px;"></p>
+<p>- 0.6g agarose </p>
 <p>- 50mL TBE 1x</p>
 <p>- 5μL SYBRsafe / 2.5μL EcoStain (light- and heat-sensitive)</p>