Difference between revisions of "Team:Pasteur Paris/Overview"
| Line 77: | Line 77: | ||
As a result of these three functions, the protein is able to specifically catch viral proteins. And with a simple marking solution, it reveals the presence of the virus. Our novel protein is integrated into a cellulose patch giving a user friendly detection device. It is rigid, easy to be manipulated, and made from a new composite biomaterial. In addition, by choosing the antibody that we fix on the protein BpA, we choose the virus that we want to detect (Zika, Chikunguya, Dengue, Yellow Fever…).</br></br> | As a result of these three functions, the protein is able to specifically catch viral proteins. And with a simple marking solution, it reveals the presence of the virus. Our novel protein is integrated into a cellulose patch giving a user friendly detection device. It is rigid, easy to be manipulated, and made from a new composite biomaterial. In addition, by choosing the antibody that we fix on the protein BpA, we choose the virus that we want to detect (Zika, Chikunguya, Dengue, Yellow Fever…).</br></br> | ||
| − | To learn more about the design of our patch with the production of this fusion protein, you can go to the <a href="https://2016.igem.org/Team:Pasteur_Paris/Science"> | + | To learn more about the design of our patch with the production of this fusion protein, you can go to the <a href="https://2016.igem.org/Team:Pasteur_Paris/Science">SCIENCE</a> section. |
</br></br> | </br></br> | ||
| Line 102: | Line 102: | ||
<img src="https://static.igem.org/mediawiki/2016/0/0b/Little_timeline_pasteur.png" width="100%" alt="image"/></img></br></br></br> | <img src="https://static.igem.org/mediawiki/2016/0/0b/Little_timeline_pasteur.png" width="100%" alt="image"/></img></br></br></br> | ||
| − | To learn more about the design of the trap and the diagnostic KIT, you can go to the <a href="https://2016.igem.org/Team:Pasteur_Paris/Moskit_devices"> | + | To learn more about the design of the trap and the diagnostic KIT, you can go to the <a href="https://2016.igem.org/Team:Pasteur_Paris/Moskit_devices">DEVICE</a> section.</br></br> |
| + | |||
| + | |||
| + | The Mos(kit)o device is easy to use and it does not require scientific expertise so it is possible to train people locally. From the set up of the trap to the analysis of the patch, the steps are simple and designed to reduce user error. For example, the quick read function has a color change component to ensure quick and accurate interpretation of results; changing from no color to color for positive results. </br></br> | ||
| + | |||
| + | We also reflected on the different stages of using our kit by establishing several scenarios on our project. | ||
| + | Let us present to you our Mos(kit)O project in <a href="https://2016.igem.org/Team:Pasteur_Paris/Scenarios">SCENARIOS</a> section! </br></br> | ||
</p> | </p> | ||
With the assistance of synthetic biology, we successfully modified E. coli, within a contained fully functioning biosafety laboratory. With it, we produce a novel fusion protein needed to create biosilica, and bind antibodies onto a cellulose support. We selected biosilica to increase rigidity of our patch and because it is completely biodegradable. The innovative design of the patch creates a multilayered matrix coated with antibodies capable of detecting a wide panel of vector-borne pathogens (like Zika, Chikunguya, Dengue, Yellow Fever…) and insecticide resistant proteins from captured mosquitoes.
As a result of these three functions, the protein is able to specifically catch viral proteins. And with a simple marking solution, it reveals the presence of the virus. Our novel protein is integrated into a cellulose patch giving a user friendly detection device. It is rigid, easy to be manipulated, and made from a new composite biomaterial. In addition, by choosing the antibody that we fix on the protein BpA, we choose the virus that we want to detect (Zika, Chikunguya, Dengue, Yellow Fever…).
To learn more about the design of our patch with the production of this fusion protein, you can go to the 
The patch is at the very heart of the kit we want to provide. The kit is made of three parts:
• A trapping system
• A detection system (the patch)
• A mapping system
The trapping system is designed to catch mosquitoes in the most effective way. It works by mimicking the signals that attract mosquito toward humans and animals. It works on three scales:
• the mosquito is attracted by the plume of CO2 emitted in a 10 to 50-meter radius
• the color contrast, that depends on the species of mosquito we target
• the moisture in the trap
When we decide to introduce a detection system against arboviruses in the environment, it is essential to take into account the safety of local people and surrounding ecosystems. That's why our new fusion protein and its cellulose support are fully biodegradable. In addition, we have thought about combining our patch with our trap in a completely sealed container that can be opened only with a key, for example. It will be necessary to train people to read the results of this test and open this container.
We imagined 2 kinds of traps. On the one hand, a stationary trap for fixed positions in urban or suburban areas, on the other, a drone based trap to span areas that are difficult to access like tropical forests. In the latter case, the drone will be programmed to come back to its base with the trap full of mosquitoes. The CO2 plume is produced by pouring vinegar on a solution of NaHCO3. We use this way to product CO2 because it is very simple and economically suitable for poor countries. Mosquitoes are attracted and trapped in a chamber.
Once the diagnosis has been performed, a trained person will come to read the results of the test kit and replace refill the system.
To learn more about the design of the trap and the diagnostic KIT, you can go to the 