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| − | <img src="http://placehold.it/800x300/d3d3d3/f2f2f2"> | + | <img src="File:BacterianBanner.png"> |
| | </div> | | </div> |
| | + | <style> |
| | + | .description { |
| | + | color: #25836E; |
| | + | font-size: 18px !important; |
| | + | } |
| | + | h2 { |
| | + | text-align:center; |
| | + | font-weight: bold; |
| | + | font-size: 200%; |
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| − | <div class="column full_size" > | + | <div class="column full_size color" > |
| − | <h2> Welcome to Team Bulgaria's Wiki page! </h2> | + | <h2> Project Description</h2> |
| − | <p>Your team has been approved and you are ready to start the iGEM season! </p> | + | <p class = "description">With the dawn of synthetic biology and the ever increasing utilization of microfluidics in experimental practice a new understanding of single-cell biology has emerged. We now realize a lot more of the inner mechanisms that regulate individual cells. |
| − | | + | </p> |
| | + | <p class = "description">Our team aims to make the detection of carbon, phosphate and nitrogen starvation in cultured cells easier and faster. In order to accomplish this we will insert different fluorescent protein genes in a bacterial plasmid. Those will be expressed together with Escherichia coli’s starvation genes and this way we will observe light in different parts of the spectrum according to the needs of the cell culture. After stimulating the transformed cells the fluorescence will be detected through signal monitoring system. The data will be used to visualize the level of expression i.e. the phase of starvation. |
| | + | </p> |
| | + | <p class = "description">We want to make a cell culture that is cheap, easy to cultivate and that shows us exactly what it lacks in its environment. If we are successful this approach could be used in many different fields including pharmacy, bio-engineering, improving the efficiency of bioreactors in general and many more. |
| | + | We have our strains and successfully made our first electroporation. |
| | + | </p> |
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| − | <h5>Before you start: </h5>
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| − | <p> Please read the following pages:</p>
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| − | <ul>
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| − | <li> <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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| − | <li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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| − | <li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
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| − | </ul>
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| − | <h5> Styling your wiki </h5>
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| − | <p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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| − | <p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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| − | <h5> Wiki template information </h5>
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| − | <p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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| − | <h5> Editing your wiki </h5>
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| − | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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| − | <p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> Click here to edit this page! </a></p>
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| − | <h5>Tips</h5>
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| − | <p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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| − | <ul>
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| − | <li>State your accomplishments! Tell people what you have achieved from the start. </li>
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| − | <li>Be clear about what you are doing and how you plan to do this.</li>
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| − | <li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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| − | <li>Make sure information is easy to find; nothing should be more than 3 clicks away. </li>
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| − | <li>Avoid using very small fonts and low contrast colors; information should be easy to read. </li>
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| − | <li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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| − | <li>Have lots of fun! </li>
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| − | </ul>
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| − | </div>
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| − | <h5>Inspiration</h5>
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| − | <p> You can also view other team wikis for inspiration! Here are some examples:</p>
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| − | <ul>
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| − | <li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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| − | <li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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| − | <li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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| − | <li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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| − | </ul>
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| − | <h5> Uploading pictures and files </h5>
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| − | <p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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| − | When you upload, set the "Destination Filename" to <code>Team:YourOfficialTeamName/NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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Project Description
With the dawn of synthetic biology and the ever increasing utilization of microfluidics in experimental practice a new understanding of single-cell biology has emerged. We now realize a lot more of the inner mechanisms that regulate individual cells.
Our team aims to make the detection of carbon, phosphate and nitrogen starvation in cultured cells easier and faster. In order to accomplish this we will insert different fluorescent protein genes in a bacterial plasmid. Those will be expressed together with Escherichia coli’s starvation genes and this way we will observe light in different parts of the spectrum according to the needs of the cell culture. After stimulating the transformed cells the fluorescence will be detected through signal monitoring system. The data will be used to visualize the level of expression i.e. the phase of starvation.
We want to make a cell culture that is cheap, easy to cultivate and that shows us exactly what it lacks in its environment. If we are successful this approach could be used in many different fields including pharmacy, bio-engineering, improving the efficiency of bioreactors in general and many more.
We have our strains and successfully made our first electroporation.
