Difference between revisions of "Team:OUC-China/Protocol"
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<ul class="nav navbar-nav" id="oucNav"> | <ul class="nav navbar-nav" id="oucNav"> | ||
<li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li> | ||
| − | <li | + | <li><a href="https://2016.igem.org/Team:OUC-China/Project">Project</a></li> |
<li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li> | ||
<li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a> | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a> | ||
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</li> | </li> | ||
<li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li> | ||
| − | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<span class="caret"></span></a> | + | <li class="dropdown active"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<span class="caret"></span></a> |
<ul class="dropdown-menu"> | <ul class="dropdown-menu"> | ||
<li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li> | <li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li> | ||
| − | <li><a href=" | + | <li><a href="#">Protocol</a></li> |
<li><a href="#">Safety</a></li> | <li><a href="#">Safety</a></li> | ||
</ul> | </ul> | ||
Revision as of 13:40, 8 October 2016
1. Elementary Operation
1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction
2. Plasmid Construction
1) Overlap PCR
2) Gibson
3) DNA cassettes
3. Systems detection
1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol
Cistrons Concerto
About
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Designed and built by @ Jasmine Chen and @ Zexin Jiao
Code licensed under Apache License v2.0
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E-mail: oucigem@163.com
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