Difference between revisions of "Team:OUC-China/Protocol"
| Line 117: | Line 117: | ||
<h3>1. Elementary Operation</h3> | <h3>1. Elementary Operation</h3> | ||
<p> | <p> | ||
| − | 1) <a href="">Luria-Bertani liquid and Luria-Bertani solid medium</a><br> | + | 1) <a href="https://static.igem.org/mediawiki/2016/1/15/T--OUC-China--Luria-Bertani_medium.pdf">Luria-Bertani liquid and Luria-Bertani solid medium</a><br> |
| − | 2) <a href="">Inoculation</a><br> | + | 2) <a href="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--Inoculation.pdf">Inoculation</a><br> |
3) <a href="">Polymerase Chain Reaction</a><br> | 3) <a href="">Polymerase Chain Reaction</a><br> | ||
| − | 4) <a href="">Gel Electrophoresis</a><br> | + | 4) <a href="https://static.igem.org/mediawiki/2016/c/cb/T--OUC-China--Gel_Electrophoresis.pdf">Gel Electrophoresis</a><br> |
5) <a href="">Restriction Enzyme Digestion</a><br> | 5) <a href="">Restriction Enzyme Digestion</a><br> | ||
| − | 6) <a href="">Gel Extraction</a><br> | + | 6) <a href="https://static.igem.org/mediawiki/2016/d/df/T--OUC-China--Gel_Extraction.pdf">Gel Extraction</a><br> |
7) <a href="">Ligation system</a><br> | 7) <a href="">Ligation system</a><br> | ||
8) <a href="">Transformation of <i>E.coli</i></a><br> | 8) <a href="">Transformation of <i>E.coli</i></a><br> | ||
| − | 9) <a href="">Conservation</a><br> | + | 9) <a href="https://static.igem.org/mediawiki/2016/8/84/T--OUC-China--Conservation.pdf">Conservation</a><br> |
10) <a href="">Sterilization</a><br> | 10) <a href="">Sterilization</a><br> | ||
11) <a href="">Plasmid extraction</a> | 11) <a href="">Plasmid extraction</a> | ||
Revision as of 14:37, 8 October 2016
1. Elementary Operation
1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction
2. Plasmid Construction
1) Overlap PCR
2) Gibson
3) DNA cassettes
3. Systems detection
1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol
Cistrons Concerto
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