Difference between revisions of "Team:OUC-China/Protocol"
| Line 123: | Line 123: | ||
5) <a href="">Restriction Enzyme Digestion</a><br> | 5) <a href="">Restriction Enzyme Digestion</a><br> | ||
6) <a href="https://static.igem.org/mediawiki/2016/d/df/T--OUC-China--Gel_Extraction.pdf">Gel Extraction</a><br> | 6) <a href="https://static.igem.org/mediawiki/2016/d/df/T--OUC-China--Gel_Extraction.pdf">Gel Extraction</a><br> | ||
| − | 7) <a href="">Ligation system</a><br> | + | 7) <a href="https://static.igem.org/mediawiki/2016/1/14/T--OUC-China--Ligation.pdf">Ligation system</a><br> |
8) <a href="">Transformation of <i>E.coli</i></a><br> | 8) <a href="">Transformation of <i>E.coli</i></a><br> | ||
9) <a href="https://static.igem.org/mediawiki/2016/8/84/T--OUC-China--Conservation.pdf">Conservation</a><br> | 9) <a href="https://static.igem.org/mediawiki/2016/8/84/T--OUC-China--Conservation.pdf">Conservation</a><br> | ||
10) <a href="">Sterilization</a><br> | 10) <a href="">Sterilization</a><br> | ||
| − | 11) <a href="">Plasmid extraction</a> | + | 11) <a href="https://static.igem.org/mediawiki/2016/1/1f/T--OUC-China--Plasmid_extraction.pdf">Plasmid extraction</a> |
</p> | </p> | ||
<h3>2. Plasmid Construction</h3> | <h3>2. Plasmid Construction</h3> | ||
Revision as of 14:39, 8 October 2016
1. Elementary Operation
1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction
2. Plasmid Construction
1) Overlap PCR
2) Gibson
3) DNA cassettes
3. Systems detection
1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol
Cistrons Concerto
About
Thanks to:
Designed and built by @ Jasmine Chen and @ Zexin Jiao
Code licensed under Apache License v2.0
Contact us
E-mail: oucigem@163.com
Follow us on Facebook@ iGEM OUC
Find us on Google Map
We are OUC-iGEM
