Difference between revisions of "Team:OUC-China/Protocol"
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1) <a href="https://static.igem.org/mediawiki/2016/1/15/T--OUC-China--Luria-Bertani_medium.pdf">Luria-Bertani liquid and Luria-Bertani solid medium</a><br> | 1) <a href="https://static.igem.org/mediawiki/2016/1/15/T--OUC-China--Luria-Bertani_medium.pdf">Luria-Bertani liquid and Luria-Bertani solid medium</a><br> | ||
2) <a href="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--Inoculation.pdf">Inoculation</a><br> | 2) <a href="https://static.igem.org/mediawiki/2016/0/0c/T--OUC-China--Inoculation.pdf">Inoculation</a><br> | ||
| − | 3) <a href="">Polymerase Chain Reaction</a><br> | + | 3) <a href="https://static.igem.org/mediawiki/2016/d/dc/T--OUC-China--Polymerase_Chain_Reaction.pdf">Polymerase Chain Reaction</a><br> |
4) <a href="https://static.igem.org/mediawiki/2016/c/cb/T--OUC-China--Gel_Electrophoresis.pdf">Gel Electrophoresis</a><br> | 4) <a href="https://static.igem.org/mediawiki/2016/c/cb/T--OUC-China--Gel_Electrophoresis.pdf">Gel Electrophoresis</a><br> | ||
| − | 5) <a href="">Restriction Enzyme Digestion</a><br> | + | 5) <a href="https://static.igem.org/mediawiki/2016/0/00/T--OUC-China--Restriction_Enzyme_Digestion.pdf">Restriction Enzyme Digestion</a><br> |
6) <a href="https://static.igem.org/mediawiki/2016/d/df/T--OUC-China--Gel_Extraction.pdf">Gel Extraction</a><br> | 6) <a href="https://static.igem.org/mediawiki/2016/d/df/T--OUC-China--Gel_Extraction.pdf">Gel Extraction</a><br> | ||
7) <a href="https://static.igem.org/mediawiki/2016/1/14/T--OUC-China--Ligation.pdf">Ligation system</a><br> | 7) <a href="https://static.igem.org/mediawiki/2016/1/14/T--OUC-China--Ligation.pdf">Ligation system</a><br> | ||
| − | 8) <a href="">Transformation of <i>E.coli</i></a><br> | + | 8) <a href="https://static.igem.org/mediawiki/2016/f/fb/T--OUC-China--Transformation_of_Escherichia_coli.pdf">Transformation of <i>E.coli</i></a><br> |
9) <a href="https://static.igem.org/mediawiki/2016/8/84/T--OUC-China--Conservation.pdf">Conservation</a><br> | 9) <a href="https://static.igem.org/mediawiki/2016/8/84/T--OUC-China--Conservation.pdf">Conservation</a><br> | ||
| − | 10) <a href="">Sterilization</a><br> | + | 10) <a href="https://static.igem.org/mediawiki/2016/c/c8/T--OUC-China--Sterilization.pdf">Sterilization</a><br> |
11) <a href="https://static.igem.org/mediawiki/2016/1/1f/T--OUC-China--Plasmid_extraction.pdf">Plasmid extraction</a> | 11) <a href="https://static.igem.org/mediawiki/2016/1/1f/T--OUC-China--Plasmid_extraction.pdf">Plasmid extraction</a> | ||
</p> | </p> | ||
| Line 132: | Line 132: | ||
<p> | <p> | ||
1) <a href="">Overlap PCR</a><br> | 1) <a href="">Overlap PCR</a><br> | ||
| − | 2) <a href="">Gibson</a><br> | + | 2) <a href="https://static.igem.org/mediawiki/2016/d/db/T--OUC-China--Gibson_aassembly.pdf">Gibson</a><br> |
| − | 3) <a href="">DNA cassettes</a> | + | 3) <a href="https://static.igem.org/mediawiki/2016/6/6a/T--OUC-China--DNA_cassettes.pdf">DNA cassettes</a> |
</p> | </p> | ||
<h3>3. Systems detection</h3> | <h3>3. Systems detection</h3> | ||
<p> | <p> | ||
| − | 1) <a href="">RNA extraction</a><br> | + | 1) <a href="https://static.igem.org/mediawiki/2016/b/b7/T--OUC-China--Total_RNA_Extraction.pdf">RNA extraction</a><br> |
| − | 2) <a href="">Reserve transcription</a><br> | + | 2) <a href="https://static.igem.org/mediawiki/2016/b/b9/T--OUC-China--Reserve_transcription.pdf">Reserve transcription</a><br> |
| − | 3) <a href="">Quantitative Real-time PCR</a><br> | + | 3) <a href="https://static.igem.org/mediawiki/2016/7/7a/T--OUC-China--Quantitative_Real-time_PCR.pdf">Quantitative Real-time PCR</a><br> |
| − | 4) <a href="">Fluorescence measurement protocol</a> | + | 4) <a href="https://static.igem.org/mediawiki/2016/2/2f/T--OUC-China--Fluorescence_measurement.pdf">Fluorescence measurement protocol</a> |
</p> | </p> | ||
</div> | </div> | ||
Revision as of 14:45, 8 October 2016
1. Elementary Operation
1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction
2. Plasmid Construction
1) Overlap PCR
2) Gibson
3) DNA cassettes
3. Systems detection
1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol
Cistrons Concerto
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