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Revision as of 12:17, 9 October 2016

Welcome to iGEM Aachen 2016

Parts

BioBrick Description
K2020000 subtilisin E gene, optimized for E. coli codon usage
K2020001 subtilisin E gene, optimized for E. coli codon usage, with leader sequence pelB
K2020002 expression system of subtilisin E in E. coli
K2020003 mutated version of expression system of subtilisin E in E. coli (S221Y)
K2020004 mutated version of expression system of subtilisin E in E. coli (S221stop)
K2020005 mutated version of expression system of subtilisin E in E. coli (Y-1W)
K2020006 mutated version of expression system of subtilisin E in E. coli (Y-1stop)
K2020020 subtilisin E gene, optimized for S. cerevisiae codon usage
K2020021 subtilisin E gene, optimized for S. cerevisiae codon usage, with the leader sequence MFalpha
K2020022 subtilisin E gene, optimized for S. cerevisiae codon usage, with the leader sequence MFalpha and His Tag
K2020023 expression system of subtilisin E in S. cerevisiae
K2020024 mutated version of expression system of subtilisin E in S. cerevisiae (S221W)
K2020025 mutated version of expression system of subtilisin E in S. cerevisiae (S221stop)
K2020026 leader sequence MFalpha (different version of the biobrick BBa_K792002)
K2020027 leader sequence MFalpha (BBa_K2020026) plus fluorescence protein RFP (BBa_E2060)
K2020028 leader sequence MFalpha (BBa_K2020026) plus fluorescence protein YFP (BBa_K165005)
K2020029 leader sequence MFalpha (BBa_K2020026) plus fluorescence protein mOrange (BBa_E2050)
K2020040 screening plasmid for incorporation of non-canonical amino acids → pRXG (twin pFRY)
K2020041 screening plasmid for incorporation of non-canonical amino acids → pRYG (twin pFRYC)
K2020042 tRNA specific for tyrosine and UAG codon in E. coli
K2020043 tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS
K2020044 tRNA synthetase specific for the ncAA AminoY and UAG codon in E. coli → AminoY-RS
K2020045 tRNA synthetase specific for the ncAA NitroY and UAG codon in E. coli → NitroY-RS
K2020046 tRNA synthetase specific for the ncAA CNF and UAG codon in E. coli → CNF-RS
K2020047 tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS
K2020050 tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS
K2020051 mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G
K2020052 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1
K2020053 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2
K2020054 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3
K2020055 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4
K2020056 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5
K2020057 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6
K2020058 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7
K2020059 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8
K2020060 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9
K2020061 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10

Basic Part

Composite Part

Part Collection