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{{OUC-China}} | {{OUC-China}} | ||
<html> | <html> | ||
+ | <head> | ||
+ | <meta charset="UTF-8"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no"> | ||
+ | <meta name="Keywords" content="关键词,关键词"> | ||
+ | <meta name="Description" content="描述"> | ||
+ | <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
+ | <link href="https://2016.igem.org/Team:OUC-China/css/bootstrap-min?action=raw&ctype=text/css" rel="stylesheet"> | ||
+ | <link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/ouc_wiki?action=raw&ctype=text/css"> | ||
+ | <link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/hp?action=raw&ctype=text/css"> | ||
+ | <script type="text/javascript" src="https://2016.igem.org/Team:OUC-China/js/jquery?action=raw&ctype=text/javascript"></script> | ||
+ | <script type="text/javascript" src="https://2016.igem.org/Team:OUC-China/js/bootstrap?action=raw&ctype=text/javascript"></script> | ||
+ | <title>Protocol</title> | ||
+ | <style type="text/css"> | ||
+ | |||
+ | .banner-top{ | ||
+ | margin-left: 12%; | ||
+ | } | ||
+ | |||
+ | @media (min-width: 992px){ | ||
+ | .work-content .col-md-4{ | ||
+ | width:25%; | ||
+ | margin-right: 8%; | ||
+ | } | ||
+ | |||
+ | } | ||
+ | #contentSub, #contentcontainer, #footer-box, #sitesub, #catlinks, #search-controls, #p-logo, .h3, .printfooter, .firstHeading, .visualClear {display: none;} /*-- hides default wiki settings --*/ | ||
− | + | #globalWrapper, #content { /*-- changes default wiki settings --*/ | |
− | + | width: 100%; | |
+ | height: 100%; | ||
+ | border: 0px; | ||
+ | background-color: transparent; | ||
+ | margin: 0px; | ||
+ | padding: 0px; | ||
+ | } | ||
+ | /* disable IGEM's style */ | ||
+ | /*#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5, #bodyContent h6 { | ||
+ | border-bottom: none; | ||
+ | padding-top: 0; | ||
+ | padding-bottom: 0; | ||
+ | margin-bottom: 0; | ||
+ | } | ||
+ | #bodyContent p { | ||
+ | font-size: 100%; | ||
+ | line-height: 1em; | ||
+ | } | ||
+ | #bodyContent li { | ||
+ | margin-bottom: 0; | ||
+ | } | ||
+ | #bodyContent a { | ||
+ | font-size: 100%; | ||
+ | }*/ | ||
+ | h1{border:0;} | ||
+ | .img_banner{margin-top:-5px;} | ||
+ | .oucBanner{height:240px;background:url('https://static.igem.org/mediawiki/2016/e/ee/T--OUC-China--banner.fw.png');margin-top:-20px;} | ||
+ | .oucNav{margin-top:16px;background:url(https://static.igem.org/mediawiki/2016/d/d0/T--OUC-China--nav-1.fw.png);border:0;} | ||
+ | .oucBrand{width:200px;height:50px;background:url(https://static.igem.org/mediawiki/2016/f/fc/T--OUC-China--nav-logo-1.fw.png);} | ||
+ | ul[class=dropdown-menu]{background:url(https://static.igem.org/mediawiki/2016/d/d0/T--OUC-China--nav-1.fw.png);} | ||
+ | .oucprotocol p a{text-decoration:none;} | ||
+ | .oucprotocol p a:hover{color:red;} | ||
+ | </style> | ||
+ | </head> | ||
+ | <body> | ||
+ | <div class="container"> | ||
+ | <nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation"> | ||
+ | <div class="container"> | ||
+ | <div class="navbar-header"> | ||
+ | <button type="button" class="navbar-toggle" data-toggle="collapse" | ||
+ | data-target="#example-navbar-collapse"> | ||
+ | <span class="sr-only">切换导航</span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | </button> | ||
+ | <a class="navbar-brand oucBrand" href="#"></a> | ||
+ | </div> | ||
+ | <div class="collapse navbar-collapse" id="example-navbar-collapse"> | ||
+ | <ul class="nav navbar-nav" id="oucNav"> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China">Home</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Project">Project</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Model">Model</a></li> | ||
+ | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="#">Basic part</a></li> | ||
+ | <li><a href="#">Composite parts</a></li> | ||
+ | <li><a href="#">Part collection</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Team">Team</a></li> | ||
+ | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Notebook">Notebook</a></li> | ||
+ | <li class="active"><a href="#">Protocol</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Safety">Safety</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Human_Practices">Human Practices</a></li> | ||
+ | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Others<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Attributions">Attributions</a></li> | ||
+ | <li><a href="https://2016.igem.org/Team:OUC-China/Collaborations">Collaborations</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </nav> | ||
+ | <div class="clearfix"></div> | ||
+ | <div class="img_banner"><img src="https://static.igem.org/mediawiki/2016/f/fe/T--OUC-China--protocol-banner.jpg" class="img-responsive" alt="protocol-banner"></div> | ||
+ | <div style="background:#EFF2F6;"> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <br> | ||
+ | <p>We have developed our own software which provides a database of stem loops. When users send the sequence to the server by uploading their own sequence or inputting a NCBI accession number, the software can find out corresponding useful stem loops and store them into the database. Moreover, when users need the protein express in quantification, for example inputting the ratio of two different proteins, the software can provide the most suitable stem loops according to the ratio. The relationship between the quantitative expression of protein and the free energy is based on <a href="https://2016.igem.org/Team:OUC-China/Model">our modeling</a>.<br/>With the help of this software/database, we can regulate the protein expression level more rationally, more precisely and more quickly. We have already created a platform/toolbox for quantitative expression using stem loops with various free energy. We believe that our platform will play a great role in prokaryote regulation field.</p> | ||
+ | <br><br><br><br> | ||
+ | </div> | ||
+ | |||
+ | <div class="oucBanner"> | ||
+ | <h3 class="text-center">Cistrons Concerto</h3> | ||
+ | </div> | ||
+ | <div class="row oucInformation" style="text-align:left"> | ||
+ | <div class="col-md-1"></div> | ||
+ | <div class="col-md-5"> | ||
+ | <b>About:</b> | ||
+ | <p style="margin-top:20px;">Thanks to:<img src="https://static.igem.org/mediawiki/2016/5/57/T--OUC-China--foot1.jpg" style="margin-left:10px;" alt="Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences"><img src="https://static.igem.org/mediawiki/2016/f/f0/T--OUC-China--foot2.jpg" style="margin-left:10px;" alt="Biolabs"></p> | ||
+ | <p style="margin-top:20px;">Designed and built by @ Jasmine Chen and @ Zexin Jiao</p> | ||
+ | <p style="margin-top:20px;">Code licensed under Apache License v4.0</p> | ||
+ | </div> | ||
+ | <div class="col-md-1"></div> | ||
+ | <div class="col-md-5"> | ||
+ | <b>Contact us:</b> | ||
+ | <p style="margin-top:20px;">E-mail: oucigem@163.com</p> | ||
+ | <p>Follow us on Facebook@ iGEM OUC<img src="https://static.igem.org/mediawiki/2016/9/94/T--OUC-China--foot3.png" style="margin-left:20px;" alt="Facebook"></p> | ||
+ | <p>Find us on Google Map</p> | ||
+ | <p>We are OUC-iGEM<img src="https://static.igem.org/mediawiki/2016/5/58/T--OUC-China--foot4.png" style="margin-left:10px;" alt="logo-one"><img src="https://static.igem.org/mediawiki/2016/9/9b/T--OUC-China--foot5.png" style="margin-left:10px;" alt="logo-two"></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div id="" class="oucBottom"> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
− | + | </body> | |
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</html> | </html> |
Revision as of 23:34, 14 October 2016
We have developed our own software which provides a database of stem loops. When users send the sequence to the server by uploading their own sequence or inputting a NCBI accession number, the software can find out corresponding useful stem loops and store them into the database. Moreover, when users need the protein express in quantification, for example inputting the ratio of two different proteins, the software can provide the most suitable stem loops according to the ratio. The relationship between the quantitative expression of protein and the free energy is based on our modeling.
With the help of this software/database, we can regulate the protein expression level more rationally, more precisely and more quickly. We have already created a platform/toolbox for quantitative expression using stem loops with various free energy. We believe that our platform will play a great role in prokaryote regulation field.
Cistrons Concerto
Thanks to:
Designed and built by @ Jasmine Chen and @ Zexin Jiao
Code licensed under Apache License v4.0
E-mail: oucigem@163.com
Follow us on Facebook@ iGEM OUC
Find us on Google Map
We are OUC-iGEM