Difference between revisions of "Team:OUC-China/Protocol"
| Line 10: | Line 10: | ||
<link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/ouc_wiki?action=raw&ctype=text/css"> | <link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/ouc_wiki?action=raw&ctype=text/css"> | ||
<link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/hp?action=raw&ctype=text/css"> | <link rel="stylesheet" href="https://2016.igem.org/Team:OUC-China/css/hp?action=raw&ctype=text/css"> | ||
| − | <script src=" | + | <script type="text/javascript" src="https://2016.igem.org/Team:OUC-China/js/jquery?action=raw&ctype=text/javascript"></script> |
| − | <script src=" | + | <script type="text/javascript" src="https://2016.igem.org/Team:OUC-China/js/bootstrap?action=raw&ctype=text/javascript"></script> |
<title>Human practice</title> | <title>Human practice</title> | ||
<style type="text/css"> | <style type="text/css"> | ||
Revision as of 09:27, 10 October 2016
1. Elementary Operation
1) Luria-Bertani liquid and Luria-Bertani solid medium
2) Inoculation
3) Polymerase Chain Reaction
4) Gel Electrophoresis
5) Restriction Enzyme Digestion
6) Gel Extraction
7) Ligation system
8) Transformation of E.coli
9) Conservation
10) Sterilization
11) Plasmid extraction
2. Plasmid Construction
1) Overlap PCR
2) Gibson
3) DNA cassettes
3. Systems detection
1) RNA extraction
2) Reserve transcription
3) Quantitative Real-time PCR
4) Fluorescence measurement protocol
Cistrons Concerto
About
Thanks to:
Designed and built by @ Jasmine Chen and @ Zexin Jiao
Code licensed under Apache License v2.0
Contact us
E-mail: oucigem@163.com
Follow us on Facebook@ iGEM OUC
Find us on Google Map
We are OUC-iGEM
