Difference between revisions of "Team:Exeter/Project"
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<p id="pp"><b>Fig.1-6</b> The average percentage viable cells for induced and uninduced samples after 6 hrs of exposure to 12 W/m<sup>2</sup> of white light. Intensity was measured using an Ocean Optics USB2000+VIS-NIR-ES spectrometer, connected to a CC3 cosine corrector with a 3.9 mm collection diameter attached to a 0.55 mm diameter optical fibre. CFU count for the control condition was treated as 100 % and viable cells calculated as a proportion of that value. CFUs were not counted above 300, any lawns were assigned the value of 300. Error bars represent the standard error of the mean.The average temperature in the light box was 38.63 ? | <p id="pp"><b>Fig.1-6</b> The average percentage viable cells for induced and uninduced samples after 6 hrs of exposure to 12 W/m<sup>2</sup> of white light. Intensity was measured using an Ocean Optics USB2000+VIS-NIR-ES spectrometer, connected to a CC3 cosine corrector with a 3.9 mm collection diameter attached to a 0.55 mm diameter optical fibre. CFU count for the control condition was treated as 100 % and viable cells calculated as a proportion of that value. CFUs were not counted above 300, any lawns were assigned the value of 300. Error bars represent the standard error of the mean.The average temperature in the light box was 38.63 ? | ||
</p> | </p> | ||
| + | <div class="row"> | ||
| + | <div class="col-xs-6" style="padding:5px 2% 5px 10%;margin:0;"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/7/78/T--Exeter--KRcont.jpg" | ||
| + | style="max-width:100%;margin:auto;display:block;"> | ||
| + | <span class="caption">Figure 1: A graph showing the mRNA amount in blue over 100 seconds and | ||
| + | the overall rate of Protein production in red over 100 seconds.</span> | ||
| + | <br> | ||
| + | <br> | ||
| + | </div> | ||
| + | <div class="row"> | ||
| + | <div class="col-xs-6" style="padding:5px 2% 5px 10%;margin:0;"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/9/9f/T--Exeter--KRDpercent2.jpg" | ||
| + | style="max-width:100%;margin:auto;display:block;"> | ||
| + | <span class="caption">Figure 1: A graph showing the mRNA amount in blue over 100 seconds and | ||
| + | the overall rate of Protein production in red over 100 seconds.</span> | ||
| + | <br> | ||
| + | <br> | ||
| + | </div> | ||
| + | <div class="row"> | ||
| + | <div class="col-xs-6" style="padding:5px 2% 5px 10%;margin:0;"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/d/d0/T--Exeter--KOcont.jpg" | ||
| + | style="max-width:100%;margin:auto;display:block;"> | ||
| + | <span class="caption">Figure 1: A graph showing the mRNA amount in blue over 100 seconds and | ||
| + | the overall rate of Protein production in red over 100 seconds.</span> | ||
| + | <br> | ||
| + | <br> | ||
| + | </div> | ||
| + | <div class="row"> | ||
| + | <div class="col-xs-6" style="padding:5px 2% 5px 10%;margin:0;"> | ||
| + | <img src="https://static.igem.org/mediawiki/2016/0/01/T--Exeter--KOpercent2.jpg" | ||
| + | style="max-width:100%;margin:auto;display:block;"> | ||
| + | <span class="caption">Figure 1: A graph showing the mRNA amount in blue over 100 seconds and | ||
| + | the overall rate of Protein production in red over 100 seconds.</span> | ||
| + | <br> | ||
| + | <br> | ||
| + | </div> | ||
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<p id="pp"><b>Fig.7-8</b> Average number of colonies after 0 h, 24 h, 120 h and 168 h. Values were averaged across three biological repeats. A max value of 300 colonies is set as any plate with more than 300 colonies was not counted and assigned the max value. All samples were induced to a final concentration of 0.2 nM IPTG. All samples were diluted 1000 times in a final volume of 4.5 ml LB. Error bars represent the standard error of the mean</p> | <p id="pp"><b>Fig.7-8</b> Average number of colonies after 0 h, 24 h, 120 h and 168 h. Values were averaged across three biological repeats. A max value of 300 colonies is set as any plate with more than 300 colonies was not counted and assigned the max value. All samples were induced to a final concentration of 0.2 nM IPTG. All samples were diluted 1000 times in a final volume of 4.5 ml LB. Error bars represent the standard error of the mean</p> | ||
Revision as of 14:36, 10 October 2016
