Line 10: | Line 10: | ||
width: 100%; | width: 100%; | ||
margin-bottom: 0; | margin-bottom: 0; | ||
+ | padding: 0; | ||
display: table; | display: table; | ||
color: white; | color: white; | ||
− | background-image: url('https://static. | + | background-image: url('https://static.pexels.com/photos/4154/clinic-doctor-health-hospital-large.jpg'); |
background-size: cover; | background-size: cover; | ||
background-position: top; | background-position: top; | ||
Line 21: | Line 22: | ||
display: table-cell; | display: table-cell; | ||
vertical-align: middle; | vertical-align: middle; | ||
+ | background-color: rgba(0, 0, 0, 0.2); | ||
} | } | ||
.parallax { | .parallax { | ||
Line 26: | Line 28: | ||
overflow-x: hidden; | overflow-x: hidden; | ||
overflow-y: auto; | overflow-y: auto; | ||
− | } | + | } |
.concept { | .concept { | ||
display: block; | display: block; | ||
Line 33: | Line 35: | ||
width: 80% | width: 80% | ||
} | } | ||
− | + | .project .container-fluid { | |
− | + | padding: 30px 25px; | |
− | + | ||
} | } | ||
− | + | .project .container-fluid .row { | |
− | + | padding-bottom: 20px; | |
− | . | + | border-bottom: 1px solid #d3d3d3; |
− | + | } | |
− | + | .project .container-fluid .row a { | |
+ | color: black; | ||
} | } | ||
− | + | .project .container-fluid .row .col-sm-4 { | |
− | + | ||
− | + | ||
− | + | ||
padding: 20px; | padding: 20px; | ||
− | |||
− | |||
− | |||
− | |||
} | } | ||
− | + | .section-image { | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
border-radius: 50%; | border-radius: 50%; | ||
− | height: | + | height: 250px; |
− | width: | + | width: 250px; |
background-color: #364C5E; | background-color: #364C5E; | ||
} | } | ||
− | + | .section-image img { | |
margin: 0 auto 0 auto; | margin: 0 auto 0 auto; | ||
− | height: | + | height: 150px; |
− | width: | + | width: 150px; |
margin-top: 25px; | margin-top: 25px; | ||
} | } | ||
− | + | .image-align { | |
padding-top: 20px; | padding-top: 20px; | ||
text-align: center; | text-align: center; | ||
− | } | + | } |
.slideanim {visibility:hidden;} | .slideanim {visibility:hidden;} | ||
.slide { | .slide { | ||
Line 110: | Line 89: | ||
} | } | ||
@media screen and (max-width: 736px) { | @media screen and (max-width: 736px) { | ||
− | |||
− | |||
} | } | ||
@media screen and (max-width: 480px) { | @media screen and (max-width: 480px) { | ||
− | } | + | } |
</style> | </style> | ||
</head> | </head> | ||
Line 132: | Line 109: | ||
<img id="prostal-logo" alt="Prostal Brand" class="featurette-image img-responsive center-block" src="https://static.igem.org/mediawiki/2016/0/0e/T--TEC-Costa_Rica--prostal_logo.png"></a> | <img id="prostal-logo" alt="Prostal Brand" class="featurette-image img-responsive center-block" src="https://static.igem.org/mediawiki/2016/0/0e/T--TEC-Costa_Rica--prostal_logo.png"></a> | ||
</div> | </div> | ||
− | |||
<div id="navbar" class="collapse navbar-collapse pull-right"> | <div id="navbar" class="collapse navbar-collapse pull-right"> | ||
<ul class="nav navbar-nav"> | <ul class="nav navbar-nav"> | ||
Line 189: | Line 165: | ||
<html> | <html> | ||
<body> | <body> | ||
− | <main class="project | + | <main class="project well"> |
<div class="jumbotron text-center parallax"> | <div class="jumbotron text-center parallax"> | ||
− | <h1 style="font-size: 50px;"> | + | <h1 style="font-size: 50px;">Project</h1> |
</div> | </div> | ||
<div class="concept"> | <div class="concept"> | ||
− | <p style="text-align: center;"> | + | <p style="text-align: center;">Prostal is a non-invasive, innovative system which aims to detect RNA and to produce a signal which is easy to read. To achieve this, we have separated our project in two main modules: I) detection of RNA and II) Signal. For the RNA detection, we chose the dCas9 (D10A, H840A) protein of <em>Streptococcus pyogenes</em>. This protein binds to the RNA and is engineered to produce a measurable signal.</p> |
− | </div> | + | </div> |
− | <h2> | + | <h2>Background</h2> |
− | <p> | + | <p>Prostate cancer (pCa) is the second most common type of cancer among men in Costa Rica, with a man dying every 27 hours in average. Yearly data since 2012 shows that an average of 373 men die every year of this disease, and 44 of every 100 000 men suffer from this illness. Globally, an estimated 300 000 men die every year of pCa, and more than a million men are diagnosed, so this is a global concern. This data is aggravated by the fact that most of deaths occur because men refuse to undergo clinical examination, which consists mainly in Digital Rectal Examination (DRE). Therefore, if a method which can detect pCa is developed in which DRE or a biopsy are not needed, the rate of deaths could be dramatically reduced, since pCa is not aggressive if properly treated. There are alternative tests, such as Prostate Specific Antigen (PSA) or RNA-PCR amplification, but the first is not really specific for cancer, and the second one is expensive and requires costly equipment. Therefore, we propose a bacterial-based system which can detect RNA and produce an easy-to-read signal.</p> |
− | < | + | <h2>Overview</h2> |
− | + | <p>ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod | |
− | + | tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, | |
− | + | quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo | |
− | + | consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse | |
− | + | cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non | |
− | + | proident, sunt in culpa qui officia deserunt mollit anim id est laborum.</p> | |
− | + | ||
− | + | <!-- Container (About Section) --> | |
− | + | <div class="container-fluid slideanim"> | |
− | + | <div class="row"> | |
− | + | <div class="col-sm-4"> | |
− | + | <div class="section-image"> | |
− | + | <div class="image-align"> | |
− | + | <a href="https://2016.igem.org/Team:TEC-Costa_Rica/Project/System"><img id="system" src="https://static.igem.org/mediawiki/2016/6/6b/T--TEC-Costa_Rica--prostal_project_system.png" class="img-responsive" alt="Project System"></a> | |
− | + | </div> | |
− | + | </div> | |
− | + | ||
</div> | </div> | ||
− | + | <div class="col-sm-8"> | |
− | + | <h2>System</h2> | |
− | + | <p>Our system was designed aiming to detect PCA3, a long non-coding RNA that works as a biomarker for prostate cancer detection, but it can be used to detect any DNA or RNA molecule. This system uses a modified dCas9 to detect an specific nucleic acid molecule and the conformational change this protein suffers when it binds to the target molecule is used to trigger the signaling mechanism.</p> | |
− | + | <br><a class="btn btn-default btn-lg" href="https://2016.igem.org/Team:TEC-Costa_Rica/Project/System">Get in Touch</a> | |
− | + | </div> | |
− | + | </div> | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</div> | </div> | ||
− | <div id=" | + | |
+ | <div class="container-fluid slideanim"> | ||
+ | <div class="row"> | ||
+ | <div class="col-sm-4 col-sm-push-8"> | ||
+ | <div class="section-image"> | ||
+ | <div class="image-align"> | ||
+ | <a href="https://2016.igem.org/Team:TEC-Costa_Rica/Project/Modelling_Simulations"><img id="modelling_simulations" src="https://static.igem.org/mediawiki/2016/1/1c/T--TEC-Costa_Rica--prostal_project_simulations.png" class="img-responsive" alt="Project Modelling & Simulations"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-sm-8 col-sm-pull-4"> | ||
+ | <h2>Modelling & Simulations</h2> | ||
+ | <p>The modelling for the Project can be classified into two categories:</p> | ||
+ | <ul> | ||
+ | <li><p>Mathematical modelling</p></li> | ||
+ | <li><p>Structural modelling</p></li> | ||
+ | </ul> | ||
+ | <br><a class="btn btn-default btn-lg" href="https://2016.igem.org/Team:TEC-Costa_Rica/Project/Modelling_Simulations">Get in Touch</a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="container-fluid slideanim"> | ||
<div class="row"> | <div class="row"> | ||
<div class="col-sm-4"> | <div class="col-sm-4"> | ||
− | + | <div class="section-image"> | |
+ | <div class="image-align"> | ||
+ | <a href="https://2016.igem.org/Team:TEC-Costa_Rica/Project/Parts"><img id="parts" src="https://static.igem.org/mediawiki/2016/1/1c/T--TEC-Costa_Rica--prostal_project_simulations.png" class="img-responsive" alt="Project Parts"></a> | ||
+ | </div> | ||
+ | </div> | ||
</div> | </div> | ||
<div class="col-sm-8"> | <div class="col-sm-8"> | ||
− | + | <h2>Parts</h2> | |
− | + | <p>Lorem ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.</p> | |
+ | <br><a class="btn btn-default btn-lg" href="https://2016.igem.org/Team:TEC-Costa_Rica/Project/Modelling_Simulations">Get in Touch</a> | ||
</div> | </div> | ||
</div> | </div> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</div> | </div> | ||
− | </main> | + | |
+ | <div class="container-fluid slideanim"> | ||
+ | <div class="row" style="border: none;"> | ||
+ | <div class="col-sm-4 col-sm-push-8"> | ||
+ | <div class="section-image"> | ||
+ | <div class="image-align"> | ||
+ | <a href="https://2016.igem.org/Team:TEC-Costa_Rica/Project/Safety"><img id="safety" src="https://static.igem.org/mediawiki/2016/5/5e/T--TEC-Costa_Rica--prostal_project_safety.png" class="img-responsive" alt="Project Safety"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-sm-8 col-sm-pull-4"> | ||
+ | <h2>Safety</h2> | ||
+ | <p>Our project is related to the detection of a human disease via a biomarker molecule found in the urine of patients with prostate cancer. Since local regulation does not allow us to work with human samples, we designed our project in a way that it can work as a proof-of-concept of a detection system and pose no risk to human health.</p> | ||
+ | <br><a class="btn btn-default btn-lg" href="https://2016.igem.org/Team:TEC-Costa_Rica/Project/Safety">Get in Touch</a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div class="container-fluid slideanim"> | ||
+ | <div class="row"> | ||
+ | <div class="col-sm-4"> | ||
+ | <div class="section-image"> | ||
+ | <div class="image-align"> | ||
+ | <a href="https://2016.igem.org/Team:TEC-Costa_Rica/Project/Collaborations"><img id="collaborations" src="https://static.igem.org/mediawiki/2016/7/76/T--TEC-Costa_Rica--prostal_project_collaborations.png" alt="Project Collaborations"></a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="col-sm-8"> | ||
+ | <h2>Collaborations</h2> | ||
+ | <p>Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. | ||
+ | Lorem ipsum dolor sit amet, consectetur adipiscing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat.</p> | ||
+ | <br><a class="btn btn-default btn-lg" href="https://2016.igem.org/Team:TEC-Costa_Rica/Project/Collaborations">Get in Touch</a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </main> | ||
<script type="text/javascript"> | <script type="text/javascript"> | ||
var system = document.getElementById('system'); | var system = document.getElementById('system'); | ||
system.addEventListener("mouseover", function() { | system.addEventListener("mouseover", function() { | ||
− | this.style.height = " | + | this.style.height = "175px"; |
− | this.style.width = " | + | this.style.width = "175px"; |
this.style.marginTop = "10px"; | this.style.marginTop = "10px"; | ||
}); | }); | ||
system.addEventListener("mouseout", function() { | system.addEventListener("mouseout", function() { | ||
− | this.style.height = " | + | this.style.height = "150px"; |
− | this.style.width = " | + | this.style.width = "150px"; |
this.style.marginTop = "25px"; | this.style.marginTop = "25px"; | ||
}); | }); | ||
var modelling_simulations = document.getElementById('modelling_simulations'); | var modelling_simulations = document.getElementById('modelling_simulations'); | ||
+ | var parts = document.getElementById('parts'); | ||
var safety = document.getElementById('safety'); | var safety = document.getElementById('safety'); | ||
var collaborations = document.getElementById('collaborations'); | var collaborations = document.getElementById('collaborations'); | ||
Line 299: | Line 292: | ||
elements.forEach(function(element) { | elements.forEach(function(element) { | ||
element.addEventListener("mouseover", function() { | element.addEventListener("mouseover", function() { | ||
− | this.style.height = " | + | this.style.height = "175px"; |
− | this.style.width = " | + | this.style.width = "175px"; |
this.style.marginTop = "5px"; | this.style.marginTop = "5px"; | ||
}); | }); | ||
element.addEventListener("mouseout", function() { | element.addEventListener("mouseout", function() { | ||
− | this.style.height = " | + | this.style.height = "150px"; |
− | this.style.width = " | + | this.style.width = "150px"; |
this.style.marginTop = "25px"; | this.style.marginTop = "25px"; | ||
}); | }); | ||
}); | }); | ||
− | </script> | + | </script> |
</body> | </body> | ||
</html> | </html> | ||
{{:Team:TEC-Costa_Rica/Template:Scripts}} | {{:Team:TEC-Costa_Rica/Template:Scripts}} | ||
{{:Team:TEC-Costa_Rica/Template:Footer}} | {{:Team:TEC-Costa_Rica/Template:Footer}} |
Revision as of 20:37, 10 October 2016
Project
Prostal is a non-invasive, innovative system which aims to detect RNA and to produce a signal which is easy to read. To achieve this, we have separated our project in two main modules: I) detection of RNA and II) Signal. For the RNA detection, we chose the dCas9 (D10A, H840A) protein of Streptococcus pyogenes. This protein binds to the RNA and is engineered to produce a measurable signal.
Background
Prostate cancer (pCa) is the second most common type of cancer among men in Costa Rica, with a man dying every 27 hours in average. Yearly data since 2012 shows that an average of 373 men die every year of this disease, and 44 of every 100 000 men suffer from this illness. Globally, an estimated 300 000 men die every year of pCa, and more than a million men are diagnosed, so this is a global concern. This data is aggravated by the fact that most of deaths occur because men refuse to undergo clinical examination, which consists mainly in Digital Rectal Examination (DRE). Therefore, if a method which can detect pCa is developed in which DRE or a biopsy are not needed, the rate of deaths could be dramatically reduced, since pCa is not aggressive if properly treated. There are alternative tests, such as Prostate Specific Antigen (PSA) or RNA-PCR amplification, but the first is not really specific for cancer, and the second one is expensive and requires costly equipment. Therefore, we propose a bacterial-based system which can detect RNA and produce an easy-to-read signal.
Overview
ipsum dolor sit amet, consectetur adipisicing elit, sed do eiusmod tempor incididunt ut labore et dolore magna aliqua. Ut enim ad minim veniam, quis nostrud exercitation ullamco laboris nisi ut aliquip ex ea commodo consequat. Duis aute irure dolor in reprehenderit in voluptate velit esse cillum dolore eu fugiat nulla pariatur. Excepteur sint occaecat cupidatat non proident, sunt in culpa qui officia deserunt mollit anim id est laborum.