Scientific Collaborations

Collaboration with Sheffield iGEM Team

We met the iGEM Team from Sheffield University at the European Experience in Paris where we presented our projects to each other. When the told us that the are using the human Lipocalin 2 (Lcn2) in order to detect the siderophors of pathogenic bacteria, we came up with the idea that we could provide the team with highly pure Lcn2 protein. This was an useful collaboration as the main PI of the Munich iGEM team 2016, Prof. Dr. Arne Skerra, is one of the leading experts in the field of lipocalins. For this reason the expression constructs and the protocols for the production of this recombinant protein are well established in the lab we are affiliated to. The main challenge for the production of the recombinant protein was that the Sheffield iGEM team needed the apo-form of the protein without the bacterial siderophor as they want to scavenge siderophors from samples and analyse the protein afterwards for their concent of siderophors. The challenge here was to produce the recombinant Protein in E. coli in a way that it is no occupied by the siderophor that is produced by most E. coli strains.

Meeting the iGEM community

Under construction. Meanwhile this:

The European Experience, Paris July 2nd to 3rd

The first big journey for our team lead us all the way to Paris, France. Following the invitation by the teams from iGEM IONIS and Evry we packed our suitcases and off we went.

And after a little climbing tour to Montmartre and maybe some Super-Last-Minute poster printing ;), we settled for the night in our shady "lodging" to be ready to showcase our project for the first time publicly.

Early in morning we headed to the event location. First objective: COFFEE,
which ended to be a great challenge than expected. Don't bring a knife (instant coffee) to a gun fight (horde of thirsty, tired students) :D

But after breakfast was aquired things started to get interesting. Dozens of awesome projects from all over Europe and even the UK!
It was great to see all those ideas and of course to be able to show our bioprinting project to the world. We also got some helpful tips and hints.
After the poster sessions was over and food was ingested, some interesting panels on ethics and entrepreneurship in synthetic biology followed before it was time for Randy to drop some final Brexit jokes and end the scientific section of the event.

The evening promised to be a great dance party in "downtown paris" including a live stream of the quarterfinal match of the European Championship between Italy and of course Germany. After this sweat-braking super-tight overly nerve-wrecking game was finally over, with the better end for us :), we hit the dance floor down in the underground car park. The midnight surprise, breathtaking fire-breather, which for some reason was interrupted by the security guard, I wonder why ;).
With this awesome party night marking the end of the European Experience we used the following Sunday to enjoy a wonderful french breakfast and explore the tourist sites of the "City of Love" before it was time to board the plane back to the lab. What a great event!

iGEM meets Marburg, Marburg August 5th to 7th

Helping other teams

iGEM experiment protocols in all languages by the METU HS Ankara iGEM team

65 roses campaign by the iGEM team Tel Hai (Israel)

Bildunterschrift

References