Difference between revisions of "Team:KoreaSonyeodul/Demonstrate"

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{{KoreaSonyeodul}}
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<html lang="en">
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<html>
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    <style>
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      #wrap {width: 60%; margin: auto;}
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      body{
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      margin: 0;
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      background: url('https://static.igem.org/mediawiki/2016/e/ee/T--KoreaSonyeodul--TitleBackground.png');
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      background-size: 100%;
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      background-repeat: no-repeat;
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      background-attachment: top;
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      .site .mainBox .mainBox1 {width: 1000px; margin-left: auto; margin-right: auto;}
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      .site {width: 100%}
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      .site h1 {
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      margin-top: 250px;
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      color: #ffffff;
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      font-size: 80px;
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      height: 35px;
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      background-color: rgba(255, 255, 255, 0.0);}
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      .site h2 {margin-top: 0; position: relative; margin-bottom: 300px; padding-bottom: 100px;
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      font-size: 50px;
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      color: #ffffff;
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      height: 30px;
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      table {text-align: center;}
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      .site .mainBox .mainBox1 {width: 1000px; margin-left: auto; margin-right: auto;}
  
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      <!--FadeIn-->
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      @import url(http://fonts.googleapis.com/css?family=Open+Sans+Condensed:300);
  
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<div class="column full_size" >
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      body {padding: 0; margin: 0; background-color: #333;}
  
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<p>Here you can describe the results of your project and your future plans. </p>
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<h5>What should this page contain?</h5>
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<ul>
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<li> Clearly and objectively describe the results of your work.</li>
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      @keyframes fadeIn { from { opacity:0; } to { opacity:1; } }
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<li> Future plans for the project </li>
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<li> Considerations for replicating the experiments </li>
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</ul>
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</div>
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      animation:fadeIn ease-in 1;
  
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      -webkit-animation-fill-mode:forwards;
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      animation-fill-mode:forwards;
  
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      -webkit-animation-duration:1s;
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      -moz-animation-duration:1s;
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      animation-duration:1s;
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      }
  
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<h5> Project Achievements </h5>
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      -moz-animation-delay: 0.7s;
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<p>You can also include a list of bullet points (and links) of the successes and failures you have had over your summer. It is a quick reference page for the judges to see what you achieved during your summer.</p>
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<ul>
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      .fade-in.three {
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<li>A list of linked bullet points of the successful results during your project</li>
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      -webkit-animation-delay: 1.6s;
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<li>A list of linked bullet points of the unsuccessful results during your project. This is about being scientifically honest. If you worked on an area for a long time with no success, tell us so we know where you put your effort.</li>
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      -moz-animation-delay: 1.6s;
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</ul>
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      animation-delay: 1.6s;
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      }
  
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</div>
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      .fade-in.four {
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      -webkit-animation-delay: 1.6s;
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      -moz-animation-delay: 1.6s;
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      animation-delay: 1.6s;
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      h1{
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      font-weight: bold; font-size: 30px; line-height: 1.3em;
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      }
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      h2{
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      font-weight: normal; font-size: 25px; line-height: 1.3em;
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      }
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      h3{
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      font-weight: normal; font-size: 20px; line-height: 1.3em;
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      }
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    </style>
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    <link href="http://netdna.bootstrapcdn.com/font-awesome/4.2.0/css/font-awesome.css" rel="stylesheet"></link>
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    <link href="https://fonts.googleapis.com/css?family=Roboto" rel="stylesheet">
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      <meta name= "viewport" content = "initial-scale=1.0, width= device-width" />
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    </link>
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  </head>
  
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  <body>
  
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<div class="column half_size" >
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    <!--Title-->
  
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<h5>Inspiration</h5>
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    <div class="box fade-in one">
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<p>See how other teams presented their results.</p>
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      <div id="" align="center">
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<ul>
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        <div id="fontlight" class="site">
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<li><a href="https://2014.igem.org/Team:TU_Darmstadt/Results/Pathway">2014 TU Darmstadt </a></li>
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          <h1>
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<li><a href="https://2014.igem.org/Team:Imperial/Results">2014 Imperial </a></li>
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            <font face="Roboto" weight="200">Demonstrate</font>
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<li><a href="https://2014.igem.org/Team:Paris_Bettencourt/Results">2014 Paris Bettencourt </a></li>
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          </h1>
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</ul>
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          <h2>
 +
            <font face="Roboto">Main Subtitle</font>
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          </h2>
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        </div>
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      </div>
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    </div>
  
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</div>
 
  
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    <!--TitleBoxes-->
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    <div id="wrap">
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      <div class="box fade-in two">
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        <h1>Results</h1>
 +
        <h2>Average Decomposition Rate Experiment</h2>
 +
        <h3>
 +
          ◆ Experiment 1 : Influence of light to one meal worm
 +
        </h3>
 +
        <h3>[OVERVIEW]</h3>
 +
        <h3>In order to understand the ideal state for the meal worms, our team measured several conditions that could affect the growth rate of the meal worms. One of them is "light". To measure the effect of light to the mealworms, we set several initial conditions. </h3>
 +
        <h3>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Meal worms are in transparent container of its area of base (20X13)cm^2<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Meal worms' size in average was (2X0.2)cm^2<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Experiment period : 5/20(Fri)~7/01 (measured every Friday)<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Meal worms are eating only polystyrene(4.00g)<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-One container is put in shade, another in light<br/>
 +
        </h3>
 +
        <h3>[KEY ACHIEVEMENTS]
 +
      </h3>
 +
        <h3>
 +
          &nbsp;&nbsp;-Meal worms become imagoes faster in light than in shade<br/><br/>
 +
        </h3>
 +
     
 +
        <h3>
 +
          <br/>◆ Experiment 2 : Duration of survival of the meal worms only relying on polystrene
 +
        </h3>
 +
        <h3>[OVERVIEW]</h3>
 +
        <h3>
 +
          In order to use the mealworm as a mean of degrading polystrene, our team needed to ensure the survival ability of mealworms relying on polystrene. To measure the possible duration of its survival, we conducted this experiment. There were 5 initial conditions.
 +
        </h3>
 +
        <h3>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Meal worms are in transparent contain of its area of base 0000cm^2<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Meal worms's size in average was (2.6X0.4)cm^2<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Experiment period : 7/01(Fri)~ (measured once in two weeks)<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Meal worms are put in shade<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Meal worms are eating only polystyrene<br/>
 +
        </h3>
 +
        <h3>[KEY ACHIEVEMENTS]</h3>
 +
        <h3>
 +
          -Imago cannot survive depending only on polystyrene<br/>
 +
          -Imagoes reproduced, and we could find about 40 larvae that were newly born<br/>
 +
          (there may be more since we couldn't separate the larvae of less than 7mm from excreta)<br/>
 +
          -Newly born larvae were gathered and grown in another transparent container in sawdust<br/><br/>
 +
        </h3>
 +
       
 +
        <h3>
 +
          <br/>◆ Experiment 3 : Decomposition Rate of Polystyrene by Mealworms
 +
        </h3>
 +
        <h3>[OVERVIEW]</h3>
 +
        <h3>
 +
          To further understand the current polystyrene decomposition rate of the meal worms, we implemented several initial conditions.
 +
        </h3>
 +
        <h3>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Group A and B each has 10 meal worms in their container<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Meal worms in Group A, B are fed only on polystyrene<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Group C has only polystyrene in its container<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Group A and C experiment started in 8/24<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-Group B experiment started in 8/25<br/>
 +
        </h3>
 +
        <h3>[KEY ACHIEVEMENTS]</h3>
 +
        <h3>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-The average decomposition rate of polystyrene by mealworms can be drew by the formula<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-The amount of polystyrene that one meal worm eats for day<br/>
 +
          &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;-The average amount of polystyrene that one meal eats for the duration of (31 days)<br/><br/>
 +
        </h3>
 +
      </div>
  
  
 +
      <!--OtherTitleBoxes-->
 +
      <div class="box fade-in three">
 +
       
 +
        <h2>
 +
          <br/><br/>Adding the PET degradation ability to the meal worms
 +
          : Recombination of DH+a and pBBRMCS2-Pcon-yfaL-PETase
 +
        </h2>
 +
        <h3>[OVERVIEW]</h3>
 +
        <h3>The e-coli bacteria, DH+alpha, which is already located inside the mealworm's gut has the ability to decompose polystryene. Our team is trying to recombine DH+alpha and pBBRMCS2-Pcon-yfaL-PETase by chemical transforming them. pBBRMCS2-Pcon-yfaL-PETase is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. From this experiment we are trying to further the mealworm’s ability to decompose plastic.</h3>
 +
        <h3>Measurement of mealworms (Weight and length)</h3>
 +
        <h3>
 +
          After feeding 40 mealworms with only plastic for 5 weeks, we measured the length and the weight of the 20 samples. The average weight is 0.177g per worm and the original length is about 30.4cm. Compared to the original length 20.2cm, it is noticeable that mealworms are possible to continue their lives with plastics as their only source of nutrient.<br/><br/>
 +
        </h3>
 +
      </div>
 +
      <div class="box fade-in four">
 +
     
 +
        <h2>
 +
          <br/>Future plans for the project</h2>
 +
        <h3>After this project of adding the PET degradation ability of mealworms, we have planned several future projects that could continue our previous one. We can synthesize the mealworm’s gut bacteria itself with PETase DNA. Then, we should modify these bacteria to allow it to survive not only in the mealworm’s gut, but also in natural environments.</h3>
 +
      </div>
 +
     
 +
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      <!--Footer-->
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    </body>
 
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Revision as of 07:17, 3 October 2016

Demonstrate

Main Subtitle

Results

Average Decomposition Rate Experiment

◆ Experiment 1 : Influence of light to one meal worm

[OVERVIEW]

In order to understand the ideal state for the meal worms, our team measured several conditions that could affect the growth rate of the meal worms. One of them is "light". To measure the effect of light to the mealworms, we set several initial conditions.

     -Meal worms are in transparent container of its area of base (20X13)cm^2
     -Meal worms' size in average was (2X0.2)cm^2
     -Experiment period : 5/20(Fri)~7/01 (measured every Friday)
     -Meal worms are eating only polystyrene(4.00g)
     -One container is put in shade, another in light

[KEY ACHIEVEMENTS]

  -Meal worms become imagoes faster in light than in shade


◆ Experiment 2 : Duration of survival of the meal worms only relying on polystrene

[OVERVIEW]

In order to use the mealworm as a mean of degrading polystrene, our team needed to ensure the survival ability of mealworms relying on polystrene. To measure the possible duration of its survival, we conducted this experiment. There were 5 initial conditions.

     -Meal worms are in transparent contain of its area of base 0000cm^2
     -Meal worms's size in average was (2.6X0.4)cm^2
     -Experiment period : 7/01(Fri)~ (measured once in two weeks)
     -Meal worms are put in shade
     -Meal worms are eating only polystyrene

[KEY ACHIEVEMENTS]

-Imago cannot survive depending only on polystyrene
-Imagoes reproduced, and we could find about 40 larvae that were newly born
(there may be more since we couldn't separate the larvae of less than 7mm from excreta)
-Newly born larvae were gathered and grown in another transparent container in sawdust


◆ Experiment 3 : Decomposition Rate of Polystyrene by Mealworms

[OVERVIEW]

To further understand the current polystyrene decomposition rate of the meal worms, we implemented several initial conditions.

     -Group A and B each has 10 meal worms in their container
     -Meal worms in Group A, B are fed only on polystyrene
     -Group C has only polystyrene in its container
     -Group A and C experiment started in 8/24
     -Group B experiment started in 8/25

[KEY ACHIEVEMENTS]

     -The average decomposition rate of polystyrene by mealworms can be drew by the formula
     -The amount of polystyrene that one meal worm eats for day
     -The average amount of polystyrene that one meal eats for the duration of (31 days)



Adding the PET degradation ability to the meal worms : Recombination of DH+a and pBBRMCS2-Pcon-yfaL-PETase

[OVERVIEW]

The e-coli bacteria, DH+alpha, which is already located inside the mealworm's gut has the ability to decompose polystryene. Our team is trying to recombine DH+alpha and pBBRMCS2-Pcon-yfaL-PETase by chemical transforming them. pBBRMCS2-Pcon-yfaL-PETase is one of a series of plasmid cloning vectors created by Joachim Messing and co-workers. From this experiment we are trying to further the mealworm’s ability to decompose plastic.

Measurement of mealworms (Weight and length)

After feeding 40 mealworms with only plastic for 5 weeks, we measured the length and the weight of the 20 samples. The average weight is 0.177g per worm and the original length is about 30.4cm. Compared to the original length 20.2cm, it is noticeable that mealworms are possible to continue their lives with plastics as their only source of nutrient.


Future plans for the project

After this project of adding the PET degradation ability of mealworms, we have planned several future projects that could continue our previous one. We can synthesize the mealworm’s gut bacteria itself with PETase DNA. Then, we should modify these bacteria to allow it to survive not only in the mealworm’s gut, but also in natural environments.