Difference between revisions of "Team:Aachen/Basic Part"

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Revision as of 15:11, 11 October 2016

Welcome to iGEM Aachen 2016

Parts

BioBrick Description
K2020000 subtilisin E gene, optimized for E. coli codon usage
K2020001 subtilisin E gene, optimized for E. coli codon usage, with leader sequence pelB
K2020002 expression system of subtilisin E in E. coli
K2020003 mutated version of expression system of subtilisin E in E. coli (S221Y)
K2020004 mutated version of expression system of subtilisin E in E. coli (S221stop)
K2020005 mutated version of expression system of subtilisin E in E. coli (Y-1W)
K2020006 mutated version of expression system of subtilisin E in E. coli (Y-1stop)
K2020020 subtilisin E gene, optimized for S. cerevisiae codon usage
K2020021 subtilisin E gene, optimized for S. cerevisiae codon usage, with the leader sequence MFalpha
K2020022 subtilisin E gene, optimized for S. cerevisiae codon usage, with the leader sequence MFalpha and His Tag
K2020023 expression system of subtilisin E in S. cerevisiae
K2020024 mutated version of expression system of subtilisin E in S. cerevisiae (S221W)
K2020025 mutated version of expression system of subtilisin E in S. cerevisiae (S221stop)
K2020026 leader sequence MFalpha (different version of the biobrick BBa_K792002)
K2020027 leader sequence MFalpha (BBa_K2020026) plus fluorescence protein RFP (BBa_E2060)
K2020028 leader sequence MFalpha (BBa_K2020026) plus fluorescence protein YFP (BBa_K165005)
K2020029 leader sequence MFalpha (BBa_K2020026) plus fluorescence protein mOrange (BBa_E2050)
K2020040 screening plasmid for incorporation of non-canonical amino acids → pRXG (twin pFRY)
K2020041 screening plasmid for incorporation of non-canonical amino acids → pRYG (twin pFRYC)
K2020042 tRNA specific for tyrosine and UAG codon in E. coli
K2020043 tRNA synthetase specific for the ncAA AzF and UAG codon in E. coli → AzF-RS
K2020044 tRNA synthetase specific for the ncAA AminoY and UAG codon in E. coli → AminoY-RS
K2020045 tRNA synthetase specific for the ncAA NitroY and UAG codon in E. coli → NitroY-RS
K2020046 tRNA synthetase specific for the ncAA CNF and UAG codon in E. coli → CNF-RS
K2020047 tRNA synthetase specific for the ncAA 5HTP and UAG codon in E. coli → 5HTP-RS
K2020050 tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS
K2020051 mutated tRNA synthetase specific for tyrosine and UAG codon in E. coli → Y-RS with Y32G
K2020052 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 1
K2020053 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 2
K2020054 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 3
K2020055 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 4
K2020056 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 5
K2020057 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 6
K2020058 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 7
K2020059 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 8
K2020060 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 9
K2020061 tRNA synthetase specific for DMNB-serine and UAG codon in E. coli → version 10

Best New Basic Part

Best New Composite Part

Parts Collection

Expression In E. coli

Basic Building Blocks


Mutated Versions


Expression In S. cerevisiae

Basic Building Blocks


Mutated Versions


Improvement of an Existing Part


Evolution of a New Synthetase

Screening System


tRNA and Synthetases