Difference between revisions of "Team:Aix-Marseille/Composite Part"

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<div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">Parts</div>
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      <!-- start section 1 -->
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    <div class="col-md-6 left">
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    <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">New biobricks using existing ones</div>
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</h2></span></span>
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    <div class="space30"></div>
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    <p class="space20"><div align="justify"><span style ="font-family:Courier New">We use existing biobrick to create new biobricks we needed:
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<ul><br/>
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<li>-T7 promoter with RBS (Bba_K525998) and optimized Laccase from <i>T.Thermophilus</i> (Bba_K863011), protein likely to be more thermostable by its origin. A tag 10xHis (Bba_K844000)is located to the end of the protein (Cterm) to enable its purification (BBa_K1601015)</li><br/>
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<li>-T7 promoter with RBS and optimized Laccase from <i>T.Thermophilus</i>, protein likely to be more thermostable by its origin. The stop codon has been deleted to facilitate a protein fusion (BBa_S05313)</li>
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</ul>
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<br/><br/><br/><br/><br/><br/><br/><br/><br/>
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                          <img src="https://static.igem.org/mediawiki/2015/3/35/Our.png" class="img-responsive" width="475" height="325">
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      <!-- start section 2 -->
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    <div class="container">
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    <div class="row">
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    <div class="col-md-6 left">
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    <div align="center"><h2 class="title wow bounce in up"><span style="color:#8E3B8C"><span style="font-family:Armalite Rifle">New biobricks we designed</div>
 
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Revision as of 19:55, 11 October 2016

Chew fight

Parts

New biobricks using existing ones

We use existing biobrick to create new biobricks we needed:

  • -T7 promoter with RBS (Bba_K525998) and optimized Laccase from T.Thermophilus (Bba_K863011), protein likely to be more thermostable by its origin. A tag 10xHis (Bba_K844000)is located to the end of the protein (Cterm) to enable its purification (BBa_K1601015)

  • -T7 promoter with RBS and optimized Laccase from T.Thermophilus, protein likely to be more thermostable by its origin. The stop codon has been deleted to facilitate a protein fusion (BBa_S05313)










New biobricks we designed

We use sequences whiche were not present in the biobrick registry to create new biobricks we needed:
  • -Cytochrome C Synechocystis sp (BBa_K1601001), a cyanobacteria that suggests a cytochrome c

  • -Cytochrome C of S.oneidensis (BBa_K1601000), a proteobacterium which can live in both environments with or without oxygen that suggests a cytochrome with high activity. The stop codon has been deleted to facilitate a protein fusion.

  • -Heme A from E.coli K12 (BBa_K1601002)that enables the heme overproduction

  • -Yeast signal CXXCH from Synechocystis sp responsible for the S.oneidensis CytC translocation into the periplasm using TAT system (BBa_S05319)

  • -Yeast signal CXXCH from Synechocystis sp responsible for the Synechocystis sp CytC translocation into the periplasm using TAT system (BBa_S05320)







Linkers we designed

We designed some linker to facilitate the meeting of the laccase and the cytochrom C

  • Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC S.oneidensis (BBa_K1601008)

  • Rigid and structured linker designed to link (or connect) Laccase T.Thermophilus and CytC P.denitrificans (BBa_K1601011)

  • Rigid and structured linker designed to link (or connect) T.Thermophilus and CytC Synechocystis sp(BBa_K1601013)

  • Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC P.denitrificans (BBa_K1601009)

  • Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC Synechocystis sp (BBa_K1601010)

  • Rigid and structured linker designed to link (or connect) Laccase E.coli and CytC S.oneidensis(BBa_K1601012)







New biobricks using existing ones

We use existing biobrick to create new biobricks we needed:
  • -Yeast signal CXXCH from Synechocystis sp responsible for the S. oneidensis CytC translocation into the periplasm using TAT system
  • -T7 promoter with RBS and Laccase E.coli (Bba_K863006) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601003)
  • -T7 promoter with RBS and Laccase T.Thermophilus (Bba_K863011) without stop codon. The stop codon has been deleted to facilitate a protein fusion.(BBa_K1601004)
  • -Cytochrome C Heme Lyase (CCHL)(BBa_K1601005)
  • -Yeast signal CXXCH (BBa_K1601006)
  • -Cytochrome C P.Denitrificans (BBa_K1601007)
  • -Cytochrome C Synechocystis sp - His Tag (BBa_S05314)
  • -Cytochrome C S.oneidensis - HisTag (BBa_S05315)






New biobricks using existing ones

We use existing biobrick to create new biobricks we needed:
  • Yeast signal CXXCH - Cytochrome C Synechocystis sp (BBa_S05316)
  • Cytochrome C P.Denitrificans - His Tag (BBa_S05317)
  • Yeast signal CXXCH - Cytochrome C S.oneidensiswithout stop codon (BBa_S05318)
  • T7 promoteur,RBS - Cytochrome C S.oneidensis - HisTag (BBa_K1601016)






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  • Aix Marseille Université
  • Marseille
  • France

Chew figth project, for the iGEM competition. See you soon in Boston !