Difference between revisions of "Team:Northwestern/06 22"

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     <h1>Wednesday, June 22<sup>nd</sup></h3>
 
     <h1>Wednesday, June 22<sup>nd</sup></h3>
 
     <h2>Agenda:</h2>
 
     <h2>Agenda:</h2>
−
 
+
<ul>
 +
      <li>Check over the GenScript grant</li>
 +
      <li>Update experiment presentation</li>
 +
      <li>Research Questions</li>
 +
      <li>Troubleshooting the transformation</li>
 +
      <li>iGEM safety sheets</li>
 +
      <li>FITC dilutions</li>
 +
      <li>Website CSS</li>
 +
    </ul>
 +
    <h2>Tasks:</h2>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Paul &amp; Sara</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Troubleshooting the transformation
 +
            <ul>
 +
              <li>Followed the competent cell protocol from the third boot camp with the cells we grew overnight</li>
 +
              <li>Placed 5 mL LB and 50 uL of cell culture into each Falcon tube, then placed in the incubator for ~3 hours</li>
 +
              <li>After making competent cells, we followed the iGEM transformation <a href="https://drive.google.com/open?id=0B9EAXnPGprpBNXFNVmJoV2FOUHc">protocol</a> using the competent cells made that day, competent cells that Kelly brought us as a control, and control competent cells with kanamycin resistance</li>
 +
              <li>With these controls, we should get a better idea of whether the transformation failure was due to the DNA volume typo on the iGEM page, bad chloramphenicol, or badly made competent cells.</li>
 +
              <li>Incubated overnight </li>
 +
            </ul>
 +
          </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sam &amp; Tasfia</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>FITC dilutions
 +
            <ul>
 +
              <li>The Ludox from the iGEM interlab kit was not suspended properly</li>
 +
              <li>Absorbance was 1.2 instead of 0.4</li>
 +
              <li>Emailed iGEM HQ asking them to resend the Ludox</li>
 +
            </ul>
 +
          </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Michelle</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Researched questions that Patrick pointed out, ClyA and LPS toxicity, as well as where in/on the body OMVs can be applied</li>
 +
          <li>Worked on the website</li>
 +
          <li>Helped with the plate reader<br>
 +
          </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Shu</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Worked on the team logo</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Tyler</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Worked with Genome Compiler and looked into designing plasmids</li>
 +
          <li>Emailed sponsors</li>
 +
          <li>More background research</li>
 +
          <li>Meeting with sponsor at Fri. 10:30 </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Sam</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Did the dilutions</li>
 +
          <li>Helped with the Genscript grant</li>
 +
        </ul>
 +
      </div>
 +
    </div>
 +
    <div class="row">
 +
      <div class="col-sm-2">
 +
        <p>Jordan</p>
 +
      </div>
 +
      <div class="col-sm-10">
 +
        <ul>
 +
          <li>Emailed CTD to set up outreach opportunities</li>
 +
          <li>Set up lab tours for high school kids on July 8, Mike and Josh’s labs</li>
 +
          <li>Helped plate </li>
 +
        </ul>
 +
      </div>
 +
    </div>
 
<footer id="nav">
 
<footer id="nav">
 
       <div class="row">
 
       <div class="row">

Revision as of 14:34, 12 October 2016

Notebook

Wednesday, June 22nd

Agenda:

  • Check over the GenScript grant
  • Update experiment presentation
  • Research Questions
  • Troubleshooting the transformation
  • iGEM safety sheets
  • FITC dilutions
  • Website CSS

Tasks:

Paul & Sara

  • Troubleshooting the transformation
    • Followed the competent cell protocol from the third boot camp with the cells we grew overnight
    • Placed 5 mL LB and 50 uL of cell culture into each Falcon tube, then placed in the incubator for ~3 hours
    • After making competent cells, we followed the iGEM transformation protocol using the competent cells made that day, competent cells that Kelly brought us as a control, and control competent cells with kanamycin resistance
    • With these controls, we should get a better idea of whether the transformation failure was due to the DNA volume typo on the iGEM page, bad chloramphenicol, or badly made competent cells.
    • Incubated overnight

Sam & Tasfia

  • FITC dilutions
    • The Ludox from the iGEM interlab kit was not suspended properly
    • Absorbance was 1.2 instead of 0.4
    • Emailed iGEM HQ asking them to resend the Ludox

Michelle

  • Researched questions that Patrick pointed out, ClyA and LPS toxicity, as well as where in/on the body OMVs can be applied
  • Worked on the website
  • Helped with the plate reader

Shu

  • Worked on the team logo

Tyler

  • Worked with Genome Compiler and looked into designing plasmids
  • Emailed sponsors
  • More background research
  • Meeting with sponsor at Fri. 10:30

Sam

  • Did the dilutions
  • Helped with the Genscript grant

Jordan

  • Emailed CTD to set up outreach opportunities
  • Set up lab tours for high school kids on July 8, Mike and Josh’s labs
  • Helped plate