Difference between revisions of "Team:TJUSLS China/Collaborations"

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                         <h3>Safety</h3>
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                         <h3>Attribution</h3>
 
                         <a href="https://2016.igem.org/wiki/index.php?title=Team:TJUSLS_China" class="active">home</a>
 
                         <a href="https://2016.igem.org/wiki/index.php?title=Team:TJUSLS_China" class="active">home</a>
 
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                 <h3>Collaborations</h3>
 
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                        <p class="link">09/05/2015 by<a href="#">Admin</a>13 comments</p>
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                        <h4><a href="#">Vestibulum iaculis</a></h4>
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                        <p>Raemuis ultricaretra gc accumsan malda or sit amet errepsm do elentu vel curslor sitmt conaiing.</p>
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                        <p class="link">19/8/2013 by<a href="#">Admin</a>10 comments</p>
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                        <h4><a href="#">Pellentesque sed dolor</a></h4>
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                        <p class="link">29/9/2015 by<a href="#">Admin</a>09 comments</p>
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                        <h4><a hrf="#">Vestibulum iaculis</a></h4>
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                        <p>Raemuis ultricaretra gc accumsan malda or sit amet errepsm do elentu vel curslor sitmt conaiing.</p>
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                             <br>
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                             <b>TJUSLS_China for Nankai_China</b><br>
 +
                            1. During the Tianjin University Begonia Science Popularization Festival, we invited the Team Nankai_China to participate in our activities. We led them to visit the campus of Tianjin University and our labs then have in-depth and friendly exchanges, laying a solid foundation for our future cooperation.
 +
                            <br>2. We provided the two parts (Plate3 6C. BBA_I13602, Plate2 3K. BBa_K561000) to the Team Nankai_China when they lost their Distribution Kit.
  
                             <div align="left" style="font-size: 25px; font-family: Caroline Golino; color: #808080"> <b>Project Design</b><br>
+
                             <br><b>Nankai_China for TJUSLS_China</b><br>
 +
                            1. The Team Nankai_China invited us to visit Nankai University during the Lotus  Festival. We did introductions of our projects at an exchange meeting with the attendance of two professors from Nankai University. In the exchange we have broaden the experimental ideas and got a lot of pertinent suggestions from professors.
 +
                            <br>2. It is really touching that the captain offered to lend us their fluorescent microscope on his own initiative while ours has been broken. It helps a lot.
  
 
                            1.  PETase is nontoxic
 
 
                            Our project is on the basis of PETase and its respective mutants.PETase is isolated from a novel bacterium,Ideonella sakaiensis 201-F6, that is able to use PET as its major energy and carbon source. Thus, we have good reasons to believe there isn't any safety problem about PETase.
 
 
                            <br>
 
                            2. Our new applications are more environment-friendly
 
 
                            About our Trainning Protein project, there is no doubt that it is environment-friendly. It not only helps to solve the plastic pollution problem, using the ezymolysis way not just landfill, incineration and chemical decomposition, but also enhances its effect.
 
 
                            <br></div>
 
                            <div align="left" style="font-size: 25px; font-family: Caroline Golino; color: #808080"> <br><br><b>Lab Work</b><br>
 
 
 
                            In order to avoid danger and train our team member, everyone accepted the biological safety test even some of our members in charge of wiki. Of course, they all qualified. What’s more, when we formally conducted experiments, every member will be supervised by our instructors and each other. If we found who conducted work nonstandardly, we will punish him/her by being on duty at that day. And all our waste are discarded safely, strictly followed stipulations of our school.
 
 
                            We chose BL21 (DE3) and Pichia Pastoris as our expressing strain, and we first constructed expression vectors in E. coli DH5alpha. In the process, use kits which are safe to extract, construct and transform our plasmids. ALL THE ORGANISM we use in our lab are safety level 1 organisms. We conducted our operations on open clean benches and all the waste contains microorganisms are sterilized before further dispose to ensure safety. According to the situation, we will choose to wear protective equipment.
 
 
                            And the following is our regulations of lab:
 
 
                            Be sure that you have worn protective equipment if necessary.
 
 
                            Be sure that you have informed the instructor of the next step of the experiment plan and gotten the permission of him.
 
 
                            Keep the super clean bench clean and operate correctly.
 
 
                            Properly handle the waste generated in the experiment.
 
 
                            Clean the table after the experiment.
 
 
                            Make the experiment record and finish experiment results in time.
 
 
                            <div align="left" style="font-size: 25px; font-family: Caroline Golino; color: #808080"> <b>Shipment</b><br>
 
 
                            To achieve safe shipment, we followed the iGEM official advice for Teams in China to send the samples to GenScript in Nanjin,China which is quick and convenient.
 
                            </div>
 
 
                         </p>
 
                         </p>
<!--                        <div id="part1">
 
                            <p>
 
                                1111111111111111111111111111111111111111111111111
 
                                PETase
 
                                PET降解酶发现于一种以PET为主要碳源的微生物(Ideonella sakaiensis 201-F6),该酶能将大分子聚合物降解为单体。表面展示技术是通过目的蛋白或多肽的编码基因与锚定蛋白的基因融合,通过融合蛋白的后翻译、折叠,将目的蛋白展示在宿主细胞壁的表面,从而获得全细胞催化剂的一种方法。我团队课题以PETase为主体,利用表面展示技术,从三个方面进行研究:
 
                                第一,通过蛋白质晶体学以及X射线衍射技术来解析PETase的结构,找到酶的催化中心和结合中心,并依据其结构特性指导突变位点的选择,从而对PETase进行定向突变以提高其降解效率和热稳定性;第二,将其进行原核(大肠杆菌)和真核(毕赤酵母)表面展示以进行全细胞酶催化反应。第三,将其与疏水蛋白在毕赤酵母中进行融合分泌,利用疏水蛋白两面性中的强疏水性为PET降解反应提供疏水环境以促进降解效率。同时,将疏水蛋白与PETase在毕赤酵母中进行共展示,利用疏水蛋白能改造细胞表面性质以适应极端环境的特点进一步提高全细胞催化剂的降解效率,打破了反应条件的限制,极大地拓宽了PETase降解反应的条件,以实现PETase的工业应用。
 
                            </p>
 
                            <img src="https://static.igem.org/mediawiki/2016/7/77/B1.jpg" alt="/">
 
                        </div>
 
                        <div id="part2">
 
                            <p>
 
                                2222222222222222222222222222222222222222222222222
 
                                PETase
 
                                PET降解酶发现于一种以PET为主要碳源的微生物(Ideonella sakaiensis 201-F6),该酶能将大分子聚合物降解为单体。表面展示技术是通过目的蛋白或多肽的编码基因与锚定蛋白的基因融合,通过融合蛋白的后翻译、折叠,将目的蛋白展示在宿主细胞壁的表面,从而获得全细胞催化剂的一种方法。我团队课题以PETase为主体,利用表面展示技术,从三个方面进行研究:
 
                                第一,通过蛋白质晶体学以及X射线衍射技术来解析PETase的结构,找到酶的催化中心和结合中心,并依据其结构特性指导突变位点的选择,从而对PETase进行定向突变以提高其降解效率和热稳定性;第二,将其进行原核(大肠杆菌)和真核(毕赤酵母)表面展示以进行全细胞酶催化反应。第三,将其与疏水蛋白在毕赤酵母中进行融合分泌,利用疏水蛋白两面性中的强疏水性为PET降解反应提供疏水环境以促进降解效率。同时,将疏水蛋白与PETase在毕赤酵母中进行共展示,利用疏水蛋白能改造细胞表面性质以适应极端环境的特点进一步提高全细胞催化剂的降解效率,打破了反应条件的限制,极大地拓宽了PETase降解反应的条件,以实现PETase的工业应用。
 
                            </p>
 
                            <img src="https://static.igem.org/mediawiki/2016/7/77/B1.jpg" alt="/">
 
                        </div>
 
                        <div id="part3">
 
                            <p>
 
                                333333333333333333333333333333333333333333333333
 
                                PETase
 
                                PET降解酶发现于一种以PET为主要碳源的微生物(Ideonella sakaiensis 201-F6),该酶能将大分子聚合物降解为单体。表面展示技术是通过目的蛋白或多肽的编码基因与锚定蛋白的基因融合,通过融合蛋白的后翻译、折叠,将目的蛋白展示在宿主细胞壁的表面,从而获得全细胞催化剂的一种方法。我团队课题以PETase为主体,利用表面展示技术,从三个方面进行研究:
 
                                第一,通过蛋白质晶体学以及X射线衍射技术来解析PETase的结构,找到酶的催化中心和结合中心,并依据其结构特性指导突变位点的选择,从而对PETase进行定向突变以提高其降解效率和热稳定性;第二,将其进行原核(大肠杆菌)和真核(毕赤酵母)表面展示以进行全细胞酶催化反应。第三,将其与疏水蛋白在毕赤酵母中进行融合分泌,利用疏水蛋白两面性中的强疏水性为PET降解反应提供疏水环境以促进降解效率。同时,将疏水蛋白与PETase在毕赤酵母中进行共展示,利用疏水蛋白能改造细胞表面性质以适应极端环境的特点进一步提高全细胞催化剂的降解效率,打破了反应条件的限制,极大地拓宽了PETase降解反应的条件,以实现PETase的工业应用。
 
                            </p>
 
                            <img src="https://static.igem.org/mediawiki/2016/7/77/B1.jpg" alt="/">
 
                        </div>
 
                        <div id="part4">
 
                            <p>
 
                                44444444444444444444444444444444444444444444444444
 
                                PETase
 
                                PET降解酶发现于一种以PET为主要碳源的微生物(Ideonella sakaiensis 201-F6),该酶能将大分子聚合物降解为单体。表面展示技术是通过目的蛋白或多肽的编码基因与锚定蛋白的基因融合,通过融合蛋白的后翻译、折叠,将目的蛋白展示在宿主细胞壁的表面,从而获得全细胞催化剂的一种方法。我团队课题以PETase为主体,利用表面展示技术,从三个方面进行研究:
 
                                第一,通过蛋白质晶体学以及X射线衍射技术来解析PETase的结构,找到酶的催化中心和结合中心,并依据其结构特性指导突变位点的选择,从而对PETase进行定向突变以提高其降解效率和热稳定性;第二,将其进行原核(大肠杆菌)和真核(毕赤酵母)表面展示以进行全细胞酶催化反应。第三,将其与疏水蛋白在毕赤酵母中进行融合分泌,利用疏水蛋白两面性中的强疏水性为PET降解反应提供疏水环境以促进降解效率。同时,将疏水蛋白与PETase在毕赤酵母中进行共展示,利用疏水蛋白能改造细胞表面性质以适应极端环境的特点进一步提高全细胞催化剂的降解效率,打破了反应条件的限制,极大地拓宽了PETase降解反应的条件,以实现PETase的工业应用。
 
                            </p>
 
                            <img src="https://static.igem.org/mediawiki/2016/7/77/B1.jpg" alt="/">
 
                        </div>
 
                        <div id="part5">
 
                            <p>
 
                                55555555555555555555555555555555555555555555555555
 
                                PETase
 
                                PET降解酶发现于一种以PET为主要碳源的微生物(Ideonella sakaiensis 201-F6),该酶能将大分子聚合物降解为单体。表面展示技术是通过目的蛋白或多肽的编码基因与锚定蛋白的基因融合,通过融合蛋白的后翻译、折叠,将目的蛋白展示在宿主细胞壁的表面,从而获得全细胞催化剂的一种方法。我团队课题以PETase为主体,利用表面展示技术,从三个方面进行研究:
 
                                第一,通过蛋白质晶体学以及X射线衍射技术来解析PETase的结构,找到酶的催化中心和结合中心,并依据其结构特性指导突变位点的选择,从而对PETase进行定向突变以提高其降解效率和热稳定性;第二,将其进行原核(大肠杆菌)和真核(毕赤酵母)表面展示以进行全细胞酶催化反应。第三,将其与疏水蛋白在毕赤酵母中进行融合分泌,利用疏水蛋白两面性中的强疏水性为PET降解反应提供疏水环境以促进降解效率。同时,将疏水蛋白与PETase在毕赤酵母中进行共展示,利用疏水蛋白能改造细胞表面性质以适应极端环境的特点进一步提高全细胞催化剂的降解效率,打破了反应条件的限制,极大地拓宽了PETase降解反应的条件,以实现PETase的工业应用。
 
                            </p>
 
                            <img src="https://static.igem.org/mediawiki/2016/7/77/B1.jpg" alt="/">
 
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                 </div>

Revision as of 13:58, 14 October 2016

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Collaborations

TJUSLS_China for Nankai_China
1. During the Tianjin University Begonia Science Popularization Festival, we invited the Team Nankai_China to participate in our activities. We led them to visit the campus of Tianjin University and our labs then have in-depth and friendly exchanges, laying a solid foundation for our future cooperation.
2. We provided the two parts (Plate3 6C. BBA_I13602, Plate2 3K. BBa_K561000) to the Team Nankai_China when they lost their Distribution Kit.
Nankai_China for TJUSLS_China
1. The Team Nankai_China invited us to visit Nankai University during the Lotus Festival. We did introductions of our projects at an exchange meeting with the attendance of two professors from Nankai University. In the exchange we have broaden the experimental ideas and got a lot of pertinent suggestions from professors.
2. It is really touching that the captain offered to lend us their fluorescent microscope on his own initiative while ours has been broken. It helps a lot.