Difference between revisions of "Team:Aix-Marseille/Design"

Revision as of 12:44, 12 October 2016

Design

Source of platinum

Platinum leaching makes a accumulation of this metal on the road side. By default, it has been prooved that plants are potentiel bioaccumulator of platinum, which is found concentrated on the roots, leaves.. Otherwise, the metal is also carried on the sewage sludge and is actually an issue regarding the recycling potencial of these sludge

Mobilisation by a siderophore

Improving recoveries of platinum and palladium from oxidized Platinum-group element ores of the Great Dyke, Zimbabwe, using the biogenic siderophore Desferrioxamine B, Dennis Kraemer & al.

In the previous article, authors made bioleaching with synthetised siderophores. By this way, they extracted approximately 80% of the total platinum found in the ore. But we were looking for a synthetic biological approach.

Pathway of the desferrioxamine B biosynthesis

"image voie de biosynthese de siderophore" Our strategy results for the suderophore mobilisation result in two main steps : - Transformation of E.Coli with an inductible plasmide holding the des cluster - Induction and production of the desferrioxamine B Then the produced siderophore would be transfered in the recuperation solution containing the platinum. Therefore, the platinum and other metal must be recruit by our purified siderophore. Though those siderophores would be import using a sowing of Streptomyces coelicolor . Then simple centrifugation and combustion will concentrate the solution in metal a first time.

Biosorption and reduction

This step aims to : - reduct of platinum ions Platine Pt14 into nanoparticules - bacterial flagellar protein adsorbs ions - the ambient reducer power reduces ions into nanoparticules [[file:T--Aix-Marseille--Flagel_agregat.jpeg.jpg]]

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-<p> The subparts were assembled using standard BioBrick Assembly.</p>
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