Biosorption and reduction using flagellin and peptides
This step aims to :
- adsorb ions on bacterial flagellar protein
- the ambient reducer power reduces ions into oxydised nanoparticules
It was shown that proteins are able to bind platinium and especially bacteria flagellum which are naturally a high platinum adsober. Furthermore, many peptides were generated based on sequences selected by phage display in order to enhance metal ions adsorpion including gold, silver and platinium.
All together, these findings incite us to use these natural properties to build a biobrick which is a high affinity binder of platinium based on E. coli and Desulfovibrio vulgaris flagellum and synthetic peptides sequences. To this end, we analyze the flagellin sequence and structural proprieties of the external part of the flagel. Then, on the part of the flagellin faced to the extarnal medium, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorbsion specificity and yield. In this way, peptide would be faced to the external medium and able to bind metallic ions. To obtain a high transcription level of this sequence, we put transcription control under a strong promotor enabling a high flagellin production.
Next, the flagellin produced will be added to the first concentrated platinum solution. Flagellin containing specific peptides will bind the free or available ions of the medium and reduce them into oxydised nanoparticules usable in industry. A simple centrifugation of the flagellin binding to the platinum allow to concentrate the metal a second time
