Difference between revisions of "Team:XJTLU-CHINA/Parts"

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<p> One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa-k1906001, BBa-k1906002) could improve the expression of Qβreplicase (BBa-k1906003) to generate our RNA random mutation pool </p>
 
<p> One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa-k1906001, BBa-k1906002) could improve the expression of Qβreplicase (BBa-k1906003) to generate our RNA random mutation pool </p>
 
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<p> Since the mutant product of Qβreplicase is in RNA form which is not stable enough so the combination of intron a (BBa-k1906004), intron b (BBa-k1906005) as well as the selective marker Kanamycin gene could store the mutant product as cDNA by reverse transcription. Once maturase (BBa-k1906000) is induced, antibiotic resistance gene Kanamycin in intron A-Kan- Intron B (BBa-k1906007 ) will be inserted into the mutant DNA for selection purpose to achieve the goal of DNA mutagenesis library construction </p>
 
<p> Since the mutant product of Qβreplicase is in RNA form which is not stable enough so the combination of intron a (BBa-k1906004), intron b (BBa-k1906005) as well as the selective marker Kanamycin gene could store the mutant product as cDNA by reverse transcription. Once maturase (BBa-k1906000) is induced, antibiotic resistance gene Kanamycin in intron A-Kan- Intron B (BBa-k1906007 ) will be inserted into the mutant DNA for selection purpose to achieve the goal of DNA mutagenesis library construction </p>
 
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Revision as of 15:53, 12 October 2016




PART NAME

PART NUMBER

Basic Part

maturase BBa-k1906000
Tu BBa-k1906001
Ts BBa-k1906002
replicase BBa-k1906003
intron a BBa-k1906004
intron b BBa-k1906005

Composite Part

akb BBa-k1906007

Part Collection 1

maturase BBa-k1906001
maturase BBa-k1906002
maturase BBa-k1906003

Function

One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa-k1906001, BBa-k1906002) could improve the expression of Qβreplicase (BBa-k1906003) to generate our RNA random mutation pool

Part Collection 2

maturase BBa-k1906000
intron a BBa-k1906004
intron b BBa-k1906005
akb BBa-k1906007

Function

Since the mutant product of Qβreplicase is in RNA form which is not stable enough so the combination of intron a (BBa-k1906004), intron b (BBa-k1906005) as well as the selective marker Kanamycin gene could store the mutant product as cDNA by reverse transcription. Once maturase (BBa-k1906000) is induced, antibiotic resistance gene Kanamycin in intron A-Kan- Intron B (BBa-k1906007 ) will be inserted into the mutant DNA for selection purpose to achieve the goal of DNA mutagenesis library construction