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Revision as of 16:20, 12 October 2016




Our Sponsors

XJTLU-CHINA IGEM 2016
    • igem@xjtlu.edu.cn

Location

Rm 363, Science Building
Xi'an Jiaotong-Liverpool University
111 Ren'ai Road, Suzhou,China
215123

Around the Web

Last Year Project

About XJTLU-CHINA

XJTLU-CHINA igem is sponsored by
Synbio, XJTLU, Snapgene, NEB


Learn More
XJTLU-CHINA igem 2016

PART NAME

PART NUMBER

Basic Part

maturase BBa-K1906000
Tu BBa-K1906001
Ts BBa-K1906002
replicase BBa-K1906003
intron a BBa-K1906004
intron b BBa-K1906005

Composite Part

akb BBa-K1906007

Part Collection 1

maturase BBa-K1906001
maturase BBa-K1906002
maturase BBa-K1906003

Function

One of Qβphage proteins, a RNA-dependent RNA polymerase named Qβreplicase has an ability to induce substitution of base pairing with no significant preference at a high mutation rate (~7 × 10-2). The two parts as cofactors Tu and Ts (BBa-K1906001, BBa-K1906002) could improve the expression of Qβreplicase (BBa-K1906003) to generate our RNA random mutation pool

Part Collection 2

maturase BBa-K1906000
intron a BBa-K1906004
intron b BBa-K1906005
akb BBa-K1906007

Function

Since the mutant product of Qβreplicase is in RNA form which is not stable enough so the combination of intron a (BBa-k1906004), intron b (BBa-k1906005) as well as the selective marker Kanamycin gene could store the mutant product as cDNA by reverse transcription. Once maturase (BBa-k1906000) is induced, antibiotic resistance gene Kanamycin in intron A-Kan- Intron B (BBa-k1906007 ) will be inserted into the mutant DNA for selection purpose to achieve the goal of DNA mutagenesis library construction