Difference between revisions of "ASIJProjectDescription"

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Upon conducting our research on plastic degradation, shortly after deciding to focus on this topic, our team came across a joint collaboration research project from Keio University and the Kyoto Institute of Technology, two prestigious universities in Japan. Their paper explained their recent research on PET degradation into Terephthalic acid and ethylene glycol using a new bacterium originating from Japan called <i>Ideonella Saikainesis</i>. We were inspired by <i>Ideonella Saikainesis</i> because it was an effective PET degrader (containing PETase) from Osaka, Japan, our current location. Considering this, we realized that the production of PETase was based on unique Japanese research, so it was only apt to center our project around this as it showcases a special (and scientifically recent) aspect of the Japanese environment. Consequently, our aim and experimental idea were born: to optimize the use of PETase and hopefully add a new Biobrick to the iGEM catalog. Subsequently, when looking at past iGEM projects of Turkey 2014, University of Washington's 2012 and Darmstadt 2012, all centered on plastic degradation, provided us with inspiration on the potential applications for our project in addition to lab protocol ideas. </h4>
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Upon conducting our research on plastic degradation, shortly after deciding to focus on this topic, our team came across a joint collaboration research project from Keio University and the Kyoto Institute of Technology, two prestigious universities in Japan. Their paper explained their recent research on PET degradation into Terephthalic acid and ethylene glycol using a new bacterium originating from Japan called <i>Ideonella Saikainesis</i>. We were inspired by <i>Ideonella Saikainesis</i> because it was an effective PET degrader (containing PETase) from Osaka, Japan, our current location. Considering this, we realized that the production of PETase was based on unique Japanese research, so it was only apt to center our project around this as it showcases a special (and scientifically recent) aspect of the Japanese environment. Consequently, our aim and experimental idea were born: to optimize the use of PETase and hopefully add a new Biobrick to the iGEM catalog. Subsequently, when looking at past iGEM projects of Turkey 2014, University of Washington 2012 and Darmstadt 2012, all centered on plastic degradation, provided us with inspiration on the potential applications for our project in addition to lab protocol ideas. </h4>
  
 
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We chose to optimize PETase as our goal to make a new type of biobrick available to future iGEM teams. We hope that this will make experiments in the following years easier to conduct. However, in order to even get to that stage, we recognized that we needed a well thought out procedure. Our team is currently working on this step. Thus far we have determined we will be using a western blot test to help us deal with the signal peptides. Currently we also have three promoters (one on the slightly weaker side and two on the stronger side) taken from the list of Anderson promoters to test in order to determine how to make PETase most efficient. Before testing such promoters though, we need to isolate evaluate each promoters' effectiveness and efficiency. This information is essential before we proceed onto our next steps and think about real world applications. Questions we may ask ourselves following this first step include: “How long will the degradation take?” and “Will the byproducts of the degradation be harmful in any way to the environment or to people?” Our aim, as stated above, is to find a faster, more efficient process to degrade plastic. Answers to such questions will be essential in seeking solutions to global environmental problems. In regards to the first question, to our current knowledge, 450 years is the timeframe for the natural biodegradation of PET. From our perspective this is much too inefficient when you consider how much PET we use daily. As a result, our experiment will focus on increasing the efficiency of the degradation of plastic through the manipulation of PETase. These are just some examples of the types of questions that we must first consider. Once our confidence in our research progresses, we can then fully focus on individual procedural steps of our project such as the Western Blot.</p>
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We chose to optimize PETase as our goal, aiming to make a new type of biobrick available to future iGEM teams. We hoped that this will make experiments in the following years easier to conduct. However, in order to even get to that stage, we recognized that we needed a well thought out procedure. Our team is currently working on this step. Thus far we have determined that we will be using a western blot test to help us deal with the signal peptides. We also have three promoters (one on the slightly weaker side and two on the stronger side) taken from the list of Anderson promoters to test in order to determine how to make PETase most efficient.  
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However, before testing such promoters, we need to isolate and evaluate each promoters' effectiveness and efficiency. This information is essential before we proceed onto our next steps and think about real world applications. Questions we may ask ourselves following this first step include: “How long will the degradation take?” and “Will the byproducts of the degradation be harmful in any way to the environment or to people?”  
 +
 
 +
Our aim, as stated above, is to find a faster, more efficient process to degrade plastic. Answers to such questions will be essential in seeking solutions to global environmental problems. In regards to the first question, to our current knowledge, 450 years is the timeframe for the natural biodegradation of PET. From our perspective, this time frame is inefficient when you consider how much PET is used daily. As a result, our experiment focuses on increasing the efficiency of the degradation of plastic through the manipulation of PETase. These are just some examples of the types of questions that we must first consider. Once our confidence in our research progresses, we can fully focus on individual procedural steps of our project such as the Western Blot test.</p>
 
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<h1 >Abstract </h1>
 
<h1 >Abstract </h1>

Revision as of 07:16, 13 October 2016

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