Composite part
Siderophore
BBa_K1951004 : DesA producer
Part Composition:
This part is composed of 3 subparts :
- Bba_I0500 : Ribosome Binding Site
- Bba_B0034 : pARA/araC inductible promoter
- Bba_K1951000 : Lysine decarboxylase
Lysine decarboxylase DesA:
Lysine descarboxylase from Streptomyces coelicolor is an enzyme from the lyase family that converts lysine to cadaverin. The enzyme realizes the carbonyl group of the lysin amino acid. Cadaverine(1,5-diaminopentane) is a primary diamine which alkaline environment. The lysine decarboxylase is an enzyme induced the synthesis of which is promoted by anaerobiosis and an acidic pH.
In bacteriology, this enzyme is sought through the middle of Moeller lysine or medium lysine Taylor.
We registered the original sequence of this subpart in the iGEM registry of standard parts (BBa_K1951000). We optimized our sequence for E.coli and ordered the synthesis by addition of an inductible promoter.
Flagellin
BBa_K1941008 : FliC E. coli producer
Part Composition:
This part is a composite part composed of 2 Biobricks :
- BBa_K1951005: flagellin C
- BBa_K880005: Strong promoter, strong RBS combination
Flagellin C from Escherichia coli
Flagellin C (FliC) protein from Escherichia coli strain is the main protein constitutive of the flagelar filament and is involved to promote bacterial swimming. This sequence is conserved in many bacterial strains. It has been demonstrated that Flagellin has the ability to adsorb precious metal such as Platinum, gold...
We made a FliC mutant by transduction using phage P1 in a E. Coli W3110 strain. Then we have complemented the FliC mutant W3110 with Bba_K151008 and performed a swimming test for every background. The result has shown that swimming was recovered into the complemented FliC mutant W3110
Part Assembly:
The subparts were assembled using standard BioBrick Assembly.
Biobrick amelioration
This biobrick has been improoved from a previous one designed by Glasgow 2014 team. Please find the link of this biobrick below :
http://parts.igem.org/Part:BBa_K1463601
Instead of Bba_J23106 and Bba_J23116, we used strong promoter, strong RBS combination for high expression levels of the flagellin. By the combination of Bba_K880005 and Bba_K1951005, we made a high flagellin expression vector able to recover swimming.
BBa_K1941009 : FliC Desulfovibrio vulgaris producer
BBa_K19410010 : CsgA Escherichia coli producer
BBa_K1941011 : DesABCD Streptomyces coelicolor producer
