Difference between revisions of "Team:Aix-Marseille/Design"

(Biosorption and reduction using flagellin and peptides)
(Mobilisation by a siderophore)
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==Mobilisation by a siderophore==
 
==Mobilisation by a siderophore==
  
In the previous article<ref>Improving recoveries of platinum and palladium from oxidized Platinum-group element ores of the Great Dyke, Zimbabwe, using the biogenic siderophore Desferrioxamine B, Dennis Kraemer & al.
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In the previous article <ref> Improving recoveries of platinum and palladium from oxidized Platinum-group element ores of the Great Dyke, Zimbabwe, using the biogenic siderophore Desferrioxamine B, Dennis Kraemer & al. </ref>, authors made bioleaching with synthetised siderophores. By this way, they extracted approximately 80% of the total platinum found in the ore. But we were looking for a synthetic biological approach.  
 
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</ref>, authors made bioleaching with synthetised siderophores. By this way, they extracted approximately 80% of the total platinum found in the ore. But we were looking for a synthetic biological approach.  
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===Pathway of the desferrioxamine B biosynthesis===
 
===Pathway of the desferrioxamine B biosynthesis===
 
[[File:T--Aix-Marseille--desABCD.jpeg|600px|center|thumb|Desferrioxamine B pathway]]  
 
[[File:T--Aix-Marseille--desABCD.jpeg|600px|center|thumb|Desferrioxamine B pathway]]  
  
Previous work showed that siderophore which are molecule secreted by bacteria to catch iron are able to catch other metals by default. Especifically, many articles showed a high affinity of Desferrioxamine B ( produce by ''Streptomyces coelicolor'') for tetravalent metal ions and more specifically platinum. These results encouraged us to make a biobrick coding the sequence corresponding to the 4 enzymes involved on the metabolic pathway of Desferrioxamine B. <i>E. coli</i> was used to produce this biobrick. Produce a gram positive bacteria pathway in a gram negative bacteria is restrictive<ref> Wandersman & Delepaire https://www.ncbi.nlm.nih.gov/pubmed/15487950  </ref> , considering the risk of toxicity for the conductress bacteria. To counter this potential issue, we regulate trasnscription using the control of an inductible promotor (pBAD/araC).   
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Previous work showed that siderophore which are molecule secreted by bacteria to catch iron are able to catch other metals by default. Especifically, many articles showed a high affinity of Desferrioxamine B ( produce by ''Streptomyces coelicolor'') for tetravalent metal ions and more specifically platinum. These results encouraged us to make a biobrick coding the sequence corresponding to the 4 enzymes involved on the metabolic pathway of Desferrioxamine B. <i>E. coli</i> was used to produce this biobrick. Produce a gram positive bacteria pathway in a gram negative bacteria is restrictive <ref> Wandersman & Delepaire https://www.ncbi.nlm.nih.gov/pubmed/15487950  </ref> , considering the risk of toxicity for the conductress bacteria. To counter this potential issue, we regulate trasnscription using the control of an inductible promotor (pBAD/araC).   
  
 
Our strategy for the siderophore mobilisation results in two main steps :
 
Our strategy for the siderophore mobilisation results in two main steps :

Revision as of 12:08, 14 October 2016