Difference between revisions of "Team:UST Beijing/Collaborations"

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<h1 class="intro-lead">Collaborations</h1>
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<h1 class="intro-lead">Safety</h1>
 
<p>We contected with team of Tsinghua university called Tsinghua-A. We come to their laboratory and did experiment with them. Also, we set together to discuss our project and show different ideas of each other. We exchanged advises which mill help us doing better in next work.</p>
 
<p>We contected with team of Tsinghua university called Tsinghua-A. We come to their laboratory and did experiment with them. Also, we set together to discuss our project and show different ideas of each other. We exchanged advises which mill help us doing better in next work.</p>
 
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<h3>Collaborations</h3>
 
<h3>Collaborations</h3>
 
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<li><a href="#part1">Project communication</a></li>
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<li><a href="#part1">Experiment plan</a></li>
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<li><a href="#part2">Discussion on the problem</a></li>
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<li><a href="#part2">How our project works</a></li>
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<li><a href="#part3">Visiting laboratory</a></li>
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<li><a href="#part3">About risk</a></li>
 
<li><a href="#part4">Summary</a></li>
 
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<p class="animate-box">The friendship between University of Science and Technology Beijing(USTB) and Tsinghua University began from the middle of last century. In 1952, Beijing iron and Steel Industry School (The predecessor of USTB) was found. It was under the support of the teachers and students in Tsinghua University that our academes could be able to set up and perfect gradually developed. Because of the deep friendship between two universities, we contacted the IGEM team of TSINGHUA University, Tsinghua-A. Thanks to the help of the members of Tsinghua-A for helping us solve some problem through our communication and learning with each other.</p>
 
  
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<img src="https://static.igem.org/mediawiki/igem.org/e/ec/T--UST_Beijing--collaboration01.png" style="width:700px;"></br>
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<div id="part1"><h2>Experiment plan</h2>
  
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<p class="animate-box">In our experiments, we also used Saccharomyces cerevisiae, Rhizopus oryzae, Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium longum and Bifidobacterium breve to ferment the notogensing root powder. All these microorganisms were purchased commericially from a supermarket and considered GRAS. We aim at breaking down the cell wall of notogensing.</p></div>
  
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<p class="animate-box">We hoped to visit the laboratory TSINGHUA University, so being the host, they introduced their IGEM project to us firstly. Most members in their team are majored in automation, so we were not familiar with the Professional NOUNs like ‘noise reduction processing’ and information theory. But through a brief description, we could be able to get the point that they were interested in the problem of noise in the process of signal transmission, they hoping to methods of mathematical modeling to display how noise can have impacts on the process of signal transmission.</p>
 
  
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<p class="animate-box">Considering most members in their team are not majored in biology, we used some simple words to explain our idea rather than biological professional nouns. We mainly introduced characteristics and application of traditional Chinese medicine, notoginseng. The idea that we use notoginseng as culture medium directly for fermenting E.coli caught their attention. Then we focused on explaining the process of our double plasmid system experiments, and talking about that the phenomena were contrary to our expectation. Their assistant were interested  in our experiments. He discussed about the feasibility of some experiments.</p>
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<div id="part2"><h2>How our project works</h2>
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<p class="animate-box">E.coli strain BL21(DE3) has been transformed with two plasmids. They could express beta-gluscosidase to hydrolyse the saccharides of saponins in notogensing to improve bioavailablity. In addition, BL21(AI) strain has been transformed with one plasmid to test expression efficiency of the beta-gluosidase. Furthermore, we used traditional methods, e.g. weak acid to hydrolyse notoginseng saponins and GRAS strains in solid fermentation, to explore bioavailablity improvement.</p></div>
  
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<img src="https://static.igem.org/mediawiki/igem.org/e/e3/T--UST_Beijing--collaboration02.jpeg" style="width:700px;"></br>
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<div id="part3"><h2>About risk</h2>
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<p class="animate-box">Risks of our experiments include escape of our engineered bacteria. To prevent the accident from happenning, we used strongest commercail disinfectants we can find to treat all experimental materials exposed to bacteria. And we designed a experiment to reconfirm the lackness of genetic stability of plasmid in our bacteria under no antibiotic culture. The result proves that the plasmid will be lost in short time if the bacteria escapes the lab culture conditions.</p>
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<p class="animate-box">If we fully develop fermented notoginseng into a real product, there will be some risks for real people. During the process of notoginseng solid fermentation, some other unwanted microorganism may pollute our product, such as A. flavus. It may pose a hidden danger for human health.</p></div>
  
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<img src="https://static.igem.org/mediawiki/igem.org/6/60/T--UST_Beijing--collaboration03.jpeg" style="width:700px;"></br>
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<div id="part4"><h2>Further comments </h2>
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<p class="animate-box">Our project focus on human health, and it will benefit all the people around the world, regardless of personal wealth. People can use our DIY method to produce their own health product of notoginseng.</p></div>
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<p class="animate-box">We brought out that we met with difficulties when synthetizing 4 gene fragments into a plasmid. The Tsinghua students gave us many practical advices. One of them is suggesting us using Gibson assembly kit. With this kit, the DNA fragments could be bound to each other by using master mix (contain 3 kinds of enzymes) in a constant temperature bath for 1 hour. However, Gibson assembly kit could only assemble the fragments less than 80 bp, while the DNA primers we need to assemble were 150bp. Thanks to team members of TSINGHUA-A, we were able to amplify our plasmid by the method of using both PCR and assembly kit.</p>
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<p class="animate-box">Meanwhile, we discussed about other problems about wiki and social practice, and we all got a satisfactory result.</p></div>
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<div id="part3"><h2>Visiting laboratory</h2>
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<p class="animate-box">There were not many researchers in their Lab when we visited, so they have times to show us around their labs and discuss about conducting experiments.</p>
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<div id="part4"><h2>Summary</h2>
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<p class="animate-box">Through this communication with fellows in Tsinghua University, we have learned much about automation. It not only expanded our horizons, but also enlightened us that the combination of biology and information theory would have a broad prospect. Besides, fellows in Tsinghua University also impressed by our creative ideas of conducting experiments. In short, it’s been a pleasant and successful communication.</p>
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Revision as of 08:50, 18 October 2016

iGEM team wiki of UST_Beijing

Safety

We contected with team of Tsinghua university called Tsinghua-A. We come to their laboratory and did experiment with them. Also, we set together to discuss our project and show different ideas of each other. We exchanged advises which mill help us doing better in next work.

Experiment plan

In our experiments, we also used Saccharomyces cerevisiae, Rhizopus oryzae, Lactobacillus casei, Lactobacillus rhamnosus, Lactobacillus acidophilus, Bifidobacterium longum and Bifidobacterium breve to ferment the notogensing root powder. All these microorganisms were purchased commericially from a supermarket and considered GRAS. We aim at breaking down the cell wall of notogensing.

How our project works

E.coli strain BL21(DE3) has been transformed with two plasmids. They could express beta-gluscosidase to hydrolyse the saccharides of saponins in notogensing to improve bioavailablity. In addition, BL21(AI) strain has been transformed with one plasmid to test expression efficiency of the beta-gluosidase. Furthermore, we used traditional methods, e.g. weak acid to hydrolyse notoginseng saponins and GRAS strains in solid fermentation, to explore bioavailablity improvement.

About risk

Risks of our experiments include escape of our engineered bacteria. To prevent the accident from happenning, we used strongest commercail disinfectants we can find to treat all experimental materials exposed to bacteria. And we designed a experiment to reconfirm the lackness of genetic stability of plasmid in our bacteria under no antibiotic culture. The result proves that the plasmid will be lost in short time if the bacteria escapes the lab culture conditions.

If we fully develop fermented notoginseng into a real product, there will be some risks for real people. During the process of notoginseng solid fermentation, some other unwanted microorganism may pollute our product, such as A. flavus. It may pose a hidden danger for human health.

Further comments

Our project focus on human health, and it will benefit all the people around the world, regardless of personal wealth. People can use our DIY method to produce their own health product of notoginseng.

北京科技大学