Difference between revisions of "Team:HUST-China"

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  <body>
 
  <body>
  
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   <div class="position_content" id="page_position_content">
     <img class="block" id="u75_img" src="https://static.igem.org/mediawiki/2016/c/ce/HUST-China_main_picture.jpeg" alt="" width="438" height="438"/>
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  <div class="clearfix grpelem" id="u81-11"><!-- content -->
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      <div class="grpelem" id="u84"><!-- simple frame --></div>
    <p id="u81-2">Bistable responses in bacterial genetic networks are an important model in synthetic biology. Case study as lac operon, molecular mechanism responsible for the lysis versus lysogeny decision in bacteriaphage γ,&nbsp; Dpp and Egfr pathways in Drosophila’s wing vein patterning and so on,all prove that bistability exists in every living process of the cell. Synbio and the iGEM coummunity are trying to analyze them and take the advantage of those delicate biological system to biuld artificial ones.Based on previous achievements about bistability in bacteria, we found something interesting to study.</p>
+
    </div>
    <p id="u81-3">&nbsp;</p>
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    <div class="clearfix grpelem" id="u85-4"><!-- content -->
    <p id="u81-5">Usually, the responses level to the external stimulation in the engineered strain depend on the stimulus intensity. What if we could build an circuit that could transform the input into the output no matter what stimulus level it is. It works like a genetic filter which could senses even a pulse of input and turns out a stable output.</p>
+
      <p>more&gt;&gt;</p>
    <p id="u81-6">&nbsp;</p>
+
    </div>
    <p id="u81-8">So this year, we plan to developing a basic tool when people want to design a genetically engineered micro-organism which could sense the pulse signal from outer environment and switch its inner stable state. Additionally, we provide eukaryotic and prokaryotic types to suits multiple applications.</p>
+
    <div class="clip_frame grpelem" id="u97"><!-- image -->
    <p>&nbsp;</p>
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      <img class="block" id="u97_img" src="https://2016.igem.org/File:Description.png" alt="" width="179" height="56"/>
  </div>
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    </div>
  <div class="grpelem" id="u82"><!-- simple frame --></div>
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     </div>
  <div class="clearfix grpelem" id="u83-4"><!-- content -->
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    <div class="clearfix colelem" id="pu75"><!-- group -->
    <p>Description</p>
+
    <div class="clip_frame grpelem" id="u75"><!-- image -->
  </div>
+
      <img class="block" id="u75_img" src="https://static.igem.org/mediawiki/2016/c/ce/HUST-China_main_picture.jpeg" alt="" width="469" height="469"/>
  <div class="grpelem" id="u84"><!-- simple frame --></div>
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    </div>
  <div class="clearfix grpelem" id="u85-4"><!-- content -->
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    <div class="clearfix grpelem" id="u81-7"><!-- content -->
    <p>more&gt;&gt;</p>
+
      <p id="u81-2">Auto-regulation parts are very commonly used regulation elements in gene networks which form kinds of biological process such as bio-oscillation, cell cycle, etc[1]. In nature, prokaryotic cells mostly employ negative feedback regulation to ensure their physiological homeostasis[2]. While in&nbsp; eukaryotic cells, they commonly regulate their homeostasis with both negative and positive feedback[3]. The positive feedback systems, which underlie bi-stable or binary response in cells, are very important and powerful parts for synthetic biological research and development. In bi-stable system, transition between two stable states could occur when the system’s input parameters change. For example, the feedback system of cI/cro in bacteria phage γ triggers a binary switch that decides the fate of cells. Considering the functionality and significance of positive feedback to universal synthetic biology applications in bi-stable or even multi-stable systems, this year, HUST-China team tries to build a set of positive feedback fundamental tool kits for synthetic biology engineers. The systems we design will not only be adaptable to any input and output, but also can change its threshold to meet the requirement from different project purpose. As the positive feedback regulation system can transform an input pulse into stable states or outputs, it can also be applied as signal filter in circuits. Additionally, to make it a competent basic tool kits, we tries to provide both prokaryotic and eukaryotic versions, for synthetic biology engineers to compare and select for further application.</p>
  </div>
+
      <p id="u81-3">&nbsp;</p>
  <div class="clearfix grpelem" id="u86-4"><!-- content -->
+
      <p id="u81-4">&nbsp;</p>
    <p>Fliter</p>
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      <p>&nbsp;</p>
 +
    </div>
 +
    <div class="clearfix grpelem" id="u89-10"><!-- content -->
 +
      <p id="u89">&nbsp;</p>
 +
      <p id="u89-3">[1] Feedback control of intercellular signaling in development. Nature, 408, 313-319.</p>
 +
      <p id="u89-5">[2] From specific gene regulation to genomic networks: a global analysis of transcriptional regulation in E.coli. BioEssays, 20, 433-440.</p>
 +
      <p id="u89-7">[3] Autoregulation of eukaryotic transcription factors. Prog. Nucleic Acid Res. Mol. Biol., 60, 133-168.</p>
 +
      <p>&nbsp;</p>
 +
    </div>
 +
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Revision as of 07:02, 30 June 2016

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Auto-regulation parts are very commonly used regulation elements in gene networks which form kinds of biological process such as bio-oscillation, cell cycle, etc[1]. In nature, prokaryotic cells mostly employ negative feedback regulation to ensure their physiological homeostasis[2]. While in  eukaryotic cells, they commonly regulate their homeostasis with both negative and positive feedback[3]. The positive feedback systems, which underlie bi-stable or binary response in cells, are very important and powerful parts for synthetic biological research and development. In bi-stable system, transition between two stable states could occur when the system’s input parameters change. For example, the feedback system of cI/cro in bacteria phage γ triggers a binary switch that decides the fate of cells. Considering the functionality and significance of positive feedback to universal synthetic biology applications in bi-stable or even multi-stable systems, this year, HUST-China team tries to build a set of positive feedback fundamental tool kits for synthetic biology engineers. The systems we design will not only be adaptable to any input and output, but also can change its threshold to meet the requirement from different project purpose. As the positive feedback regulation system can transform an input pulse into stable states or outputs, it can also be applied as signal filter in circuits. Additionally, to make it a competent basic tool kits, we tries to provide both prokaryotic and eukaryotic versions, for synthetic biology engineers to compare and select for further application.

 

 

 

 

[1] Feedback control of intercellular signaling in development. Nature, 408, 313-319.

[2] From specific gene regulation to genomic networks: a global analysis of transcriptional regulation in E.coli. BioEssays, 20, 433-440.

[3] Autoregulation of eukaryotic transcription factors. Prog. Nucleic Acid Res. Mol. Biol., 60, 133-168.