Difference between revisions of "Team:Kyoto/Description"

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<p>Even though Norovirus (NoV) remains the leading cause of food poisoning in the developed countries, its cultivation system has yet to come. Thus, when detecting NoV with high sensitivity as in food inspection, one must depend on a qRT-PCR of the virus RNA coupled with special virus concentration method. As a more economical alternative to this method, iGEM Kyoto is developing a biodevice that utilizes recombinant E.coli expressing a surface-displayed NoV antibody (only the variable fragment) and cellulose binding domain to detect miniscule amount of NoVs. We are also thinking of utilizing the said E.coli to treat/prevent NoV infection by directly introducing them to the patient small intestine.
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As we have been working on this project since last summer, we already have constructed the plasmids that codes for the expression of the surface-displayed variable fragment of NoV antibody and the surface-displayed cellulose binding domain, respectively. Additionally, through both western blotting and scanning electron microscopy, we have confirmed the specific affinity of NoV to the E.coli expressing the said recombinant proteins. This summer, we plan on measuring the affinity of cellulose to the surface-displayed cellulose binding domain, expressing the two surface-displayed proteins simultaneously, and ultimately testing the functionality, reliability, and practicality of our NoV detection system.</p>
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<h3>★  ALERT! </h3>
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<p>This page is used by the judges to evaluate your team for the<a href="https://2016.igem.org/Judging/Medals"> improve a previous part or project gold medal criterion</a>. </p>
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<p> Delete this box in order to be evaluated for this medal. See more information at <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions"> Instructions for Pages for awards</a>.</p>
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<p>Tell us about your project, describe what moves you and why this is something important for your team.</p>
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<h5>What should this page contain?</h5>
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<li> A clear and concise description of your project.</li>
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<li>A detailed explanation of why your team chose to work on this particular project.</li>
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<li>References and sources to document your research.</li>
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<li>Use illustrations and other visual resources to explain your project.</li>
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<h5>Advice on writing your Project Description</h5>
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We encourage you to put up a lot of information and content on your wiki, but we also encourage you to include summaries as much as possible. If you think of the sections in your project description as the sections in a publication, you should try to be consist, accurate and unambiguous in your achievements.
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Judges like to read your wiki and know exactly what you have achieved. This is how you should think about these sections; from the point of view of the judge evaluating you at the end of the year.
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<h5>References</h5>
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<p>iGEM teams are encouraged to record references you use during the course of your research. They should be posted somewhere on your wiki so that judges and other visitors can see how you thought about your project and what works inspired you.</p>
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<h5>Inspiration</h5>
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<p>See how other teams have described and presented their projects: </p>
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<li><a href="https://2014.igem.org/Team:Imperial/Project"> Imperial</a></li>
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<li><a href="https://2014.igem.org/Team:UC_Davis/Project_Overview"> UC Davis</a></li>
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<li><a href="https://2014.igem.org/Team:SYSU-Software/Overview">SYSU Software</a></li>
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Revision as of 14:56, 11 October 2016

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