Difference between revisions of "Team:Aix-Marseille/Design"
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[[File:T--Aix-Marseille--Flagel_platine.jpeg|200px|left|thumb|Adsorption of the platinum on the flagellum, Deplenche & al. 2008]] | [[File:T--Aix-Marseille--Flagel_platine.jpeg|200px|left|thumb|Adsorption of the platinum on the flagellum, Deplenche & al. 2008]] | ||
| − | It was shown that proteins are able to bind platinium and especially bacterial flagellums which are naturally a high platinum adsober <ref>Capeness & al. 2015, http://eprints.nottingham.ac.uk/27979/1/Michael%20Capeness%20-%20Thesis%20-%20PDF.pdf</ref><ref>Deplanche & al., 2007 http://onlinelibrary.wiley.com/doi/10.1002/bit.21688/abstract. </ref> Furthermore, many peptides were generated based on sequences selected by phage display in order to enhance metal ions | + | It was shown that proteins are able to bind platinium and especially bacterial flagellums which are naturally a high platinum adsober <ref>Capeness & al. 2015, http://eprints.nottingham.ac.uk/27979/1/Michael%20Capeness%20-%20Thesis%20-%20PDF.pdf</ref><ref>Deplanche & al., 2007 http://onlinelibrary.wiley.com/doi/10.1002/bit.21688/abstract. </ref> Furthermore, many peptides were generated based on sequences selected by phage display in order to enhance metal ions adsorption including gold, silver platinum and a plenty of other metals.<ref>Uratu Ozgur Safak Seker & al., 2011, http://www.mdpi.com/1420-3049/16/2/1426.</ref> |
| − | + | Hence the potential of our process to be performed with other metals. | |
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This step aims to : | This step aims to : | ||
| − | * adsorb ions on bacterial | + | * adsorb ions on bacterial flagella protein |
| − | * the ambient reducer power reduces ions into | + | * the ambient reducer power reduces ions into oxidized nano particles |
| − | All together, these findings incite us to use these natural properties to build a | + | All together, these findings incite us to use these natural properties to build a biobricks which is a high affinity binder of platinum based on <i>E. coli</i> and <i>Desulfovibrio vulgaris</i> flagellum and synthetic peptides sequences. To this end, we analyze the flagella sequence and structural proprieties of the external part of the flagella. Then, on the part of the flagellin faced to the external medium, an insertion restriction site will be inserted. Then specific precious metal peptides would be added using this insertion site to increase the level of adsorption specificity and yield. In this way, peptide would be faced to the external medium and able to bind metallic ions. To obtain a high transcription level of this sequence, we put transcription control under a strong promotor enabling a high flagellin production. |
[[File:T--Aix-Marseille--flagel_captation.jpeg|500px|center|thumb|Adsorption of the platinum on the flagellin]] | [[File:T--Aix-Marseille--flagel_captation.jpeg|500px|center|thumb|Adsorption of the platinum on the flagellin]] | ||
Next, the flagellin produced will be added to the first concentrated platinum solution. Flagellin containing specific peptides will bind the free or available ions of the medium and reduce them into oxydised nanoparticules usable in industry. A simple centrifugation of the flagellin binding to the platinum allow to concentrate the metal a second time. | Next, the flagellin produced will be added to the first concentrated platinum solution. Flagellin containing specific peptides will bind the free or available ions of the medium and reduce them into oxydised nanoparticules usable in industry. A simple centrifugation of the flagellin binding to the platinum allow to concentrate the metal a second time. | ||
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