Difference between revisions of "Team:Slovenia/Protease signaling/Light dependent mediator"
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<h1 class = "ui centered dividing header"><span class="section">nbsp;</span>Light-depended mediator</h1> | <h1 class = "ui centered dividing header"><span class="section">nbsp;</span>Light-depended mediator</h1> | ||
<div class = "ui segment"> | <div class = "ui segment"> | ||
| + | <p>We designed and successfully tested three light inducible split proteases: CIBN/CRY2PHR light inducible split TEV protease, | ||
| + | CIBN/CRY2PHR light inducible split PPVp and CIBN/CRY2PHR light inducible split TEVpE. | ||
| + | </p> | ||
| + | </div> | ||
| + | <div class = "ui segment"> | ||
<h4>Introduction</h4> | <h4>Introduction</h4> | ||
<p>In the recent years, light has been extensively explored as a trigger signal for activation of different biological processes. Small molecules and other chemical | <p>In the recent years, light has been extensively explored as a trigger signal for activation of different biological processes. Small molecules and other chemical | ||
Revision as of 18:05, 15 October 2016
nbsp;Light-depended mediator
We designed and successfully tested three light inducible split proteases: CIBN/CRY2PHR light inducible split TEV protease, CIBN/CRY2PHR light inducible split PPVp and CIBN/CRY2PHR light inducible split TEVpE.
Introduction
In the recent years, light has been extensively explored as a trigger signal for activation of different biological processes. Small molecules and other chemical signals lack spatial resolution and their temporal resolution is limited by the time required for the cell permeation. In comparison, induction by light as developed by the optogenetics offers many advantages. It is fast as well as inexpensive and allows for excellent spatial, temporal and dose-dependent control.
