Wednesday, September 7th
Transformation Results:
No visible colonies on our Cas9 ligation. The gel screen showed a faint band of the correct size, so we should have seen colonies. We suspect that our transformation efficiency is low.
Figure 1: Cas9 ligation
Figure 2: Positive Gibson control
Figure 3: Transformation positive control
Figure 4: Cas9+ SS Gibson
Tasks:
Michelle
- Ran gel screen on Cas9 restriction digest
- Really faint bands
- Ligation had 3 bands, were really hard to see
- pSB1C3 size, Cas9 size, and one above that
Sam
- Worked on pamphlet
