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+ | <meta charset="UTF-8"> | ||
+ | <meta name="viewport" content="width=device-width, initial-scale=1, maximum-scale=1, user-scalable=no"> | ||
+ | <meta name="Keywords" content="关键词,关键词"> | ||
+ | <meta name="Description" content="描述"> | ||
+ | <!-- 使IE用最高模式渲染页面 --> | ||
+ | <meta http-equiv="X-UA-Compatible" content="IE=edge"> | ||
+ | <!-- 新 Bootstrap 核心 CSS 文件 --> | ||
+ | <link rel="stylesheet" href="css/bootstrap.min.css"> | ||
+ | <link rel="stylesheet" type="text/css" href="css/ouc-wiki.css"> | ||
+ | <link rel="stylesheet" type="text/css" href="css/index.css"> | ||
+ | <!-- jQuery文件。务必在bootstrap.min.js 之前引入 --> | ||
+ | <script src="js/jquery.js"></script> | ||
+ | <!-- 最新的 Bootstrap 核心 JavaScript 文件 --> | ||
+ | <script src="js/bootstrap.min.js"></script> | ||
+ | <title>index </title> | ||
+ | <style type="text/css"> | ||
+ | .oucNav{background:url('image/ouc-nav-1.fw.png');border:0;} | ||
+ | .oucBrand{width:200px;height:50px;background:url('image/ouc-nav-logo-1.fw.png');} | ||
+ | ul[class=dropdown-menu]{background:url('image/ouc-nav-1.fw.png');} | ||
+ | </style> | ||
+ | </head> | ||
+ | <body> | ||
+ | <div class="container"> | ||
+ | <nav class="oucNav navbar navbar-default navbar-fixed-top" role="navigation"> | ||
+ | <div class="container"> | ||
+ | <div class="navbar-header"> | ||
+ | <button type="button" class="navbar-toggle" data-toggle="collapse" | ||
+ | data-target="#example-navbar-collapse"> | ||
+ | <span class="sr-only">切换导航</span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | <span class="icon-bar"></span> | ||
+ | </button> | ||
+ | <a class="navbar-brand oucBrand" href="#"></a> | ||
+ | </div> | ||
+ | <div class="collapse navbar-collapse" id="example-navbar-collapse"> | ||
+ | <ul class="nav navbar-nav" id="oucNav"> | ||
+ | <li class="active"><a href="#">Home</a></li> | ||
+ | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Project<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="#">Overview</a></li> | ||
+ | <li><a href="#">Background</a></li> | ||
+ | <li><a href="#">Design</a></li> | ||
+ | <li><a href="#">Protocol</a></li> | ||
+ | <li><a href="#">Result</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li><a href="#">Model</a></li> | ||
+ | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Parts<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="#">Basic part</a></li> | ||
+ | <li><a href="#">Composite parts</a></li> | ||
+ | <li><a href="#">Part collection</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Attribution<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="#">Support</a></li> | ||
+ | <li><a href="#">Collaboration</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Team<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="#">Members</a></li> | ||
+ | <li><a href="#">Instructors</a></li> | ||
+ | <li><a href="#">Advisors</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Notebook<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="#">Journal</a></li> | ||
+ | <li><a href="#">Safety</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | <li class="dropdown"><a class="dropdown-toggle" data-toggle="dropdown" href="#">Human Practice<span class="caret"></span></a> | ||
+ | <ul class="dropdown-menu"> | ||
+ | <li><a href="#">Education</a></li> | ||
+ | <li><a href="#">Communication</a></li> | ||
+ | <li><a href="#">Publicity</a></li> | ||
+ | <li><a href="#">Product Design</a></li> | ||
+ | <li><a href="#">Laws and regulations</a></li> | ||
+ | </ul> | ||
+ | </li> | ||
+ | </ul> | ||
+ | </div> | ||
+ | </div> | ||
+ | </nav> | ||
+ | <div id="carousel-example-generic" class="carousel slide" data-ride="carousel"> | ||
+ | <!-- Indicators --> | ||
+ | <ol class="carousel-indicators"> | ||
+ | <li data-target="#carousel-example-generic" data-slide-to="0" class="active"></li> | ||
+ | <li data-target="#carousel-example-generic" data-slide-to="1"></li> | ||
+ | <li data-target="#carousel-example-generic" data-slide-to="2"></li> | ||
+ | <li data-target="#carousel-example-generic" data-slide-to="3"></li> | ||
+ | <li data-target="#carousel-example-generic" data-slide-to="4"></li> | ||
+ | </ol> | ||
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− | </div> | + | |
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− | </div> | + | |
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− | + | ||
+ | <!-- Controls --> | ||
+ | <a class="left carousel-control" href="#carousel-example-generic" role="button" data-slide="prev"> | ||
+ | <span class="glyphicon glyphicon-chevron-left" aria-hidden="true"></span> | ||
+ | <span class="sr-only"></span> | ||
+ | </a> | ||
+ | <a class="right carousel-control" href="#carousel-example-generic" role="button" data-slide="next"> | ||
+ | <span class="glyphicon glyphicon-chevron-right" aria-hidden="true"></span> | ||
+ | <span class="sr-only"></span> | ||
+ | </a> | ||
+ | </div> | ||
+ | <div class="oucAbstract row"> | ||
+ | <div id="" class="col-xs-5"> | ||
+ | <img src="image/ouc-ab-icon1.png" class="img-responsive"> | ||
+ | </div> | ||
+ | <div id="" class="col-xs-2"> | ||
+ | <h1 class="text-center">Abstract</h1> | ||
+ | </div> | ||
+ | <div id="" class="col-xs-5"> | ||
+ | <img src="image/ouc-ab-icon2.png" class="img-responsive"> | ||
+ | </div> | ||
+ | <div class="text-center"> | ||
+ | <p class="text-center">As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, wecan decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.</p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="oucMovie"> | ||
+ | <img src="image/ouc-movie-1.fw.png"> | ||
+ | </div> | ||
+ | <div class="oucMenu row"> | ||
+ | <div class="col-sm-4"> | ||
+ | <a href="#"><img src="image/ouc-menu-icon-1.fw.png" class="img-responsive"><p class="text-center">Purpose</p></a> | ||
+ | </div> | ||
+ | <div class="col-sm-4"> | ||
+ | <a href="#"><img src="image/ouc-menu-icon-2.fw.png" class="img-responsive"><p class="text-center">Principle</p></a> | ||
+ | </div> | ||
+ | <div class="col-sm-4"> | ||
+ | <a href="#"><img src="image/ouc-menu-icon-3.fw.png" class="img-responsive"><p class="text-center">Potential</p></a> | ||
+ | </div> | ||
+ | <div class="clearfix"></div> | ||
+ | <div class="col-sm-4"> | ||
+ | <a href="#"><img src="image/ouc-menu-icon-4.fw.png" class="img-responsive"><p class="text-center">Attrbution</p></a> | ||
+ | </div> | ||
+ | <div class="col-sm-4"> | ||
+ | <a href="#"><img src="image/ouc-menu-icon-5.fw.png" class="img-responsive"><p class="text-center">Team</p></a> | ||
+ | </div> | ||
+ | <div class="col-sm-4"> | ||
+ | <a href="#"><img src="image/ouc-menu-icon-6.fw.png" class="img-responsive"><p class="text-center">Human Practice</p></a> | ||
+ | </div> | ||
+ | <div class="clearfix"></div> | ||
+ | </div> | ||
+ | <div class="oucMenuBottom"> | ||
+ | <h1 class="text-center">Let’s take a fantastic journey!</h1> | ||
+ | <a href="#"><p class="text-center">Check in</p></a> | ||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="oucBanner"> | ||
+ | <h3 class="text-center">Cistrons Concerto</h3> | ||
+ | </div> | ||
+ | <div class="row oucInformation"> | ||
+ | <div class="col-md-7"> | ||
+ | <b>About</b> | ||
+ | <p>Thanks to:<i><img src="image/ouc-footer-1.fw.png"></i><i><img src="image/ouc-footer-2.fw.png"></i></p> | ||
+ | <p>Designed and built by @ Jasmine Chen and @ Zexin Jiao</p> | ||
+ | <p>Code licensed under Apache License v2.0</p> | ||
+ | </div> | ||
+ | <div class="col-md-5"> | ||
+ | <b>Contact us</b> | ||
+ | <p>E-mail: oucigem@163.com</p> | ||
+ | <p>Follow us on Facebook@ iGEM OUC<i><img src="image/ouc-footer-3.fw.png"></i></p> | ||
+ | <p>Find us on Google Map</p> | ||
+ | <p>We are OUC-iGEM<i><img src="image/ouc-footer-4.fw.png"></i><i><img src="image/ouc-footer-5.fw.png"></i><i><img src="image/ouc-footer-6.fw.png"></i></p> | ||
+ | </div> | ||
+ | </div> | ||
+ | <div class="oucBottom"> | ||
+ | </div> | ||
+ | </div> | ||
+ | </div> | ||
+ | </body> | ||
</html> | </html> |
Revision as of 09:58, 1 October 2016
![](image/ouc-ab-icon1.png)
Abstract
![](image/ouc-ab-icon2.png)
As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, wecan decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.
![](image/ouc-movie-1.fw.png)
Let’s take a fantastic journey!
Check in
Cistrons Concerto
Thanks to:
Designed and built by @ Jasmine Chen and @ Zexin Jiao
Code licensed under Apache License v2.0
E-mail: oucigem@163.com
Follow us on Facebook@ iGEM OUC
Find us on Google Map
We are OUC-iGEM