Difference between revisions of "Team:OUC-China"

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{{OUC-China}}
 
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<h3 style="font-size:24px; color:#00afd1;" align="center">Project Description</h3>
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It has been proved that in <i>Clostridium cellulolyticum</i>, there is a kind of post-transcriptional regulation called selective RNA processing and stabilization (SRPS), which describes how stem-loops make a difference to mRNA level in a polycistron.
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As we all know, stem-loops which have lower folding free energy (∆G) than most oligonucleotides. And mRNA with stem-loops on 3’ or 5’ end often more stable than those without stem-loops. In some extent, the lower (∆G) of stem-loop is, the more stable corresponding mRNA is.
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Our team would like to design a series of stem-loops followed by a putative RNase E site and put them in intergenic region of a polycistron. We hope to decouple the expression level of upstream and downstream genes by this design though they are under the control of the same one promoter. And finally we would give a series of pithy regulation parts for others so that they can have another more choice.
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<h2> Welcome to iGEM 2016! </h2>
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<p>Your team has been approved and you are ready to start the iGEM season! </p>
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<h5>Before you start: </h5>
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<p> Please read the following pages:</p>
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<li>  <a href="https://2016.igem.org/Requirements">Requirements page </a> </li>
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<li> <a href="https://2016.igem.org/Wiki_How-To">Wiki Requirements page</a></li>
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<li> <a href="https://2016.igem.org/Resources/Template_Documentation"> Template Documentation </a></li>
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<h5> Styling your wiki </h5>
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<p>You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.</p>
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<p>While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.</p>
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<h5> Wiki template information </h5>
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<p>We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the <a href="https://2016.igem.org/Judging/Pages_for_Awards/Instructions">Pages for awards</a> link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!</p>
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<h5> Editing your wiki </h5>
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<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p>
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<p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p>
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<h5>Tips</h5>
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<p>This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started: </p>
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<li>State your accomplishments! Tell people what you have achieved from the start. </li>
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<li>Be clear about what you are doing and how you plan to do this.</li>
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<li>You have a global audience! Consider the different backgrounds that your users come from.</li>
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<li>Make sure information is easy to find; nothing should be more than 3 clicks away.  </li>
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<li>Avoid using very small fonts and low contrast colors; information should be easy to read.  </li>
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<li>Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the <a href="https://2016.igem.org/Calendar">iGEM 2016 calendar</a> </li>
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<li>Have lots of fun! </li>
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<h5>Inspiration</h5>
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<p> You can also view other team wikis for inspiration! Here are some examples:</p>
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<li> <a href="https://2014.igem.org/Team:SDU-Denmark/"> 2014 SDU Denmark </a> </li>
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<li> <a href="https://2014.igem.org/Team:Aalto-Helsinki">2014 Aalto-Helsinki</a> </li>
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<li> <a href="https://2014.igem.org/Team:LMU-Munich">2014 LMU-Munich</a> </li>
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<li> <a href="https://2014.igem.org/Team:Michigan"> 2014 Michigan</a></li>
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<li> <a href="https://2014.igem.org/Team:ITESM-Guadalajara">2014 ITESM-Guadalajara </a></li>
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<li> <a href="https://2014.igem.org/Team:SCU-China"> 2014 SCU-China </a></li>
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<h5> Uploading pictures and files </h5>
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<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br />
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When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p>
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UPLOAD FILES
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<h1 class="text-center">Abstract</h1>
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<p class="text-center">As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, wecan decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.</p>
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<div class="col-sm-4">
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<a href="#"><img src="image/ouc-menu-icon-1.fw.png" class="img-responsive"><p class="text-center">Purpose</p></a>
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</div>
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<div class="col-sm-4">
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<a href="#"><img src="image/ouc-menu-icon-2.fw.png" class="img-responsive"><p class="text-center">Principle</p></a>
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<a href="#"><img src="image/ouc-menu-icon-3.fw.png" class="img-responsive"><p class="text-center">Potential</p></a>
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<div class="clearfix"></div>
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<div class="col-sm-4">
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<a href="#"><img src="image/ouc-menu-icon-4.fw.png" class="img-responsive"><p class="text-center">Attrbution</p></a>
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<div class="col-sm-4">
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<a href="#"><img src="image/ouc-menu-icon-5.fw.png" class="img-responsive"><p class="text-center">Team</p></a>
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</div>
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<div class="col-sm-4">
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<a href="#"><img src="image/ouc-menu-icon-6.fw.png" class="img-responsive"><p class="text-center">Human Practice</p></a>
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</div>
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<div class="clearfix"></div>
  
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</div>
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<div class="oucMenuBottom">
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<h1 class="text-center">Let’s take a fantastic journey!</h1>
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<a href="#"><p class="text-center">Check in</p></a>
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</div>
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<div class="row">
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<div class="oucBanner">
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<h3 class="text-center">Cistrons Concerto</h3>
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</div>
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<div class="row oucInformation">
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<div class="col-md-7">
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<b>About</b>
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<p>Thanks to:<i><img src="image/ouc-footer-1.fw.png"></i><i><img src="image/ouc-footer-2.fw.png"></i></p>
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<p>Designed and built by @ Jasmine Chen and @ Zexin Jiao</p>
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<p>Code licensed under Apache License v2.0</p>
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</div>
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<div class="col-md-5">
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<b>Contact us</b>
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<p>E-mail: oucigem@163.com</p>
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<p>Follow us on Facebook@ iGEM OUC<i><img src="image/ouc-footer-3.fw.png"></i></p>
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<p>Find us on Google Map</p>
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<p>We are OUC-iGEM<i><img src="image/ouc-footer-4.fw.png"></i><i><img src="image/ouc-footer-5.fw.png"></i><i><img src="image/ouc-footer-6.fw.png"></i></p>
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Revision as of 09:58, 1 October 2016

index

Abstract

As is known, once forming stem-loops, the oligonucleotides will be more stable than the single-stranded ones. And mRNA with stem-loop at its 3’ or 5’ end often get a longer lifetime than the linear one owe to the stem-loop’s resistance to exonuclease. Our team tend to design a series of stem-loops each followed by the same endonuclease site and are transcribed as one polycistron. Once digested by endonuclease and seperate into several independent fragments, cistrons with different ∆G stem-loops will get different stability, thus influence the amount of expressed proteins. In this way, wecan decouple the expression level of upstream and downstream genes of the same operon by simply designing different stem-loops. Futhermore, with quantitative ∆G of stem-loops, we even can achieve the ratio expression of target proteins. It is a creative regulating method and we attempt to provide a series of standard regulation parts for others.

Purpose

Principle

Potential

Attrbution

Team

Human Practice

Let’s take a fantastic journey!

Check in

Cistrons Concerto

About

Thanks to:

Designed and built by @ Jasmine Chen and @ Zexin Jiao

Code licensed under Apache License v2.0

Contact us

E-mail: oucigem@163.com

Follow us on Facebook@ iGEM OUC

Find us on Google Map

We are OUC-iGEM