Difference between revisions of "Team:Hannover"
| Line 11: | Line 11: | ||
</div> | </div> | ||
| + | <h3>★ Enable the usage of TAL-effector proteins in-vitro </h3> | ||
| + | <p>TAL (Transcription Activator Like) –effector proteins are a new possibility for genetic engineering. Due to a special, repeating sequence of amino acids, a so-called repeat domain, TAL-effectors can easily bind to a certain DNA sequence and perform various functions. | ||
| + | </p><p> | ||
| + | Originally, those proteins were discovered in Xanthomonas. Those bacteria use TAL-effectors to specifically regulate host genes. After decoding the amino-acid-code of TAL-effectors, genetic changes can be generated. In this way, DNA-fragments can be replaced and precisely cut or foreign DNA can be inserted. | ||
| + | </p><p> | ||
| + | TAL-effector proteins offer a significant advantage compared to usual procedures e.g. with restriction enzymes: Trough an easy change of the amino acid sequence, the protein can be customized to any DNA sequence. The function of TAL-effectors could be proven in vivo in cell cultures and also in animals. However, they show a high instability outside living organisms. This instability leads to the problem that the purification of TAL-effectors as well as the in-vitro application in the lab is difficult to perform. For this reason, TAL-effector proteins are excluded from a huge field of application, because a lot of genetic work usually takes place “in test tubes”. | ||
| + | </p><p> | ||
| + | Our aim is to develop a circular TAL-effector with the help of a linker in order to stabilize the protein. Thereby, TAL-effectors could be utilized on a daily basis and enable new techniques of genetic engineering in the lab. | ||
| + | </p> | ||
<div class="column half_size" > | <div class="column half_size" > | ||
| Line 42: | Line 51: | ||
<h5> Editing your wiki </h5> | <h5> Editing your wiki </h5> | ||
<p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p> | <p>On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world! </p> | ||
| − | <p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> | + | <p> <a href="https://2016.igem.org/wiki/index.php?title=Team:Example&action=edit"> </a>Use WikiTools - Edit in the black menu bar to edit this page</p> |
</div> | </div> | ||
| Line 78: | Line 87: | ||
<h5> Uploading pictures and files </h5> | <h5> Uploading pictures and files </h5> | ||
<p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br /> | <p> You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name. <br /> | ||
| − | When you upload, set the "Destination Filename" to <code> | + | When you upload, set the "Destination Filename" to <br><code>T--YourOfficialTeamName--NameOfFile.jpg</code>. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)</p> |
Revision as of 18:29, 30 June 2016
Welcome to iGEM 2016!
Your team has been approved and you are ready to start the iGEM season!
★ Enable the usage of TAL-effector proteins in-vitro
TAL (Transcription Activator Like) –effector proteins are a new possibility for genetic engineering. Due to a special, repeating sequence of amino acids, a so-called repeat domain, TAL-effectors can easily bind to a certain DNA sequence and perform various functions.
Originally, those proteins were discovered in Xanthomonas. Those bacteria use TAL-effectors to specifically regulate host genes. After decoding the amino-acid-code of TAL-effectors, genetic changes can be generated. In this way, DNA-fragments can be replaced and precisely cut or foreign DNA can be inserted.
TAL-effector proteins offer a significant advantage compared to usual procedures e.g. with restriction enzymes: Trough an easy change of the amino acid sequence, the protein can be customized to any DNA sequence. The function of TAL-effectors could be proven in vivo in cell cultures and also in animals. However, they show a high instability outside living organisms. This instability leads to the problem that the purification of TAL-effectors as well as the in-vitro application in the lab is difficult to perform. For this reason, TAL-effector proteins are excluded from a huge field of application, because a lot of genetic work usually takes place “in test tubes”.
Our aim is to develop a circular TAL-effector with the help of a linker in order to stabilize the protein. Thereby, TAL-effectors could be utilized on a daily basis and enable new techniques of genetic engineering in the lab.
Before you start:
Please read the following pages:
Styling your wiki
You may style this page as you like or you can simply leave the style as it is. You can easily keep the styling and edit the content of these default wiki pages with your project information and completely fulfill the requirement to document your project.
While you may not win Best Wiki with this styling, your team is still eligible for all other awards. This default wiki meets the requirements, it improves navigability and ease of use for visitors, and you should not feel it is necessary to style beyond what has been provided.
Wiki template information
We have created these wiki template pages to help you get started and to help you think about how your team will be evaluated. You can find a list of all the pages tied to awards here at the Pages for awards link. You must edit these pages to be evaluated for medals and awards, but ultimately the design, layout, style and all other elements of your team wiki is up to you!
Editing your wiki
On this page you can document your project, introduce your team members, document your progress and share your iGEM experience with the rest of the world!
Use WikiTools - Edit in the black menu bar to edit this page
Tips
This wiki will be your team’s first interaction with the rest of the world, so here are a few tips to help you get started:
- State your accomplishments! Tell people what you have achieved from the start.
- Be clear about what you are doing and how you plan to do this.
- You have a global audience! Consider the different backgrounds that your users come from.
- Make sure information is easy to find; nothing should be more than 3 clicks away.
- Avoid using very small fonts and low contrast colors; information should be easy to read.
- Start documenting your project as early as possible; don’t leave anything to the last minute before the Wiki Freeze. For a complete list of deadlines visit the iGEM 2016 calendar
- Have lots of fun!
Inspiration
You can also view other team wikis for inspiration! Here are some examples:
Uploading pictures and files
You can upload your pictures and files to the iGEM 2016 server. Remember to keep all your pictures and files within your team's namespace or at least include your team's name in the file name.
When you upload, set the "Destination Filename" to T--YourOfficialTeamName--NameOfFile.jpg. (If you don't do this, someone else might upload a different file with the same "Destination Filename", and your file would be erased!)
