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<p id="pp">All samples from the ministat were tested using the KillerRed, KillerOrange protocol found <a href="#KRKOProt"> | <p id="pp">All samples from the ministat were tested using the KillerRed, KillerOrange protocol found <a href="#KRKOProt"> | ||
here</a>. Glycerol stocks were made of the samples taken at each time interval, testing was done using these glycerol stocks.</p> | here</a>. Glycerol stocks were made of the samples taken at each time interval, testing was done using these glycerol stocks.</p> | ||
− | <p id="pp"><b>Fig. | + | <p id="pp"><b>Fig.1,2</b> Average number of colonies after 0 h, 24 h, 120 h and 168 h of continuous culture. Values were |
averaged across three biological repeats. A max value of 300 colonies is set as any plate with more than 300 colonies was | averaged across three biological repeats. A max value of 300 colonies is set as any plate with more than 300 colonies was | ||
not counted and assigned the max value. All samples were induced to a final concentration of 0.2 nM IPTG. All samples were | not counted and assigned the max value. All samples were induced to a final concentration of 0.2 nM IPTG. All samples were | ||
diluted 1000 times in a final volume of 4.5 ml LB. Error bars represent the standard error of the mean</p> | diluted 1000 times in a final volume of 4.5 ml LB. Error bars represent the standard error of the mean</p> | ||
− | <p id="pp"> <b>Fig. | + | <p id="pp"> <b>Fig. 3,4</b> Data from Fig.7,8 represented as percentage viable cells over time. 100% viable is given |
when the CFU count for the kill switch condition equaled the control. Error bars represent the standard error of the mean.</p> | when the CFU count for the kill switch condition equaled the control. Error bars represent the standard error of the mean.</p> | ||
<div class="row"> | <div class="row"> | ||
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<img src="https://static.igem.org/mediawiki/2016/7/78/T--Exeter--KRcont.jpg" | <img src="https://static.igem.org/mediawiki/2016/7/78/T--Exeter--KRcont.jpg" | ||
style="max-width:100%;margin:auto;display:block;"> | style="max-width:100%;margin:auto;display:block;"> | ||
− | <span class="caption">Fig. | + | <span class="caption">Fig. 1. Comparison of CFUs formed by KillerRed exposed to light and kept in the dark.</span> |
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<img src="https://static.igem.org/mediawiki/2016/9/9f/T--Exeter--KRDpercent2.jpg" | <img src="https://static.igem.org/mediawiki/2016/9/9f/T--Exeter--KRDpercent2.jpg" | ||
style="max-width:100%;margin:auto;display:block;"> | style="max-width:100%;margin:auto;display:block;"> | ||
− | <span class="caption">Fig. | + | <span class="caption">Fig. 2. Percentage viable cells of KillerRed exposed to light.</span> |
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<img src="https://static.igem.org/mediawiki/2016/d/d0/T--Exeter--KOcont.jpg" | <img src="https://static.igem.org/mediawiki/2016/d/d0/T--Exeter--KOcont.jpg" | ||
style="max-width:100%;margin:auto;display:block;"> | style="max-width:100%;margin:auto;display:block;"> | ||
− | <span class="caption">Fig. | + | <span class="caption">Fig. 3. Comparison of CFUS of KillerOrange exposed to light and in the dark.</span> |
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<img src="https://static.igem.org/mediawiki/2016/0/01/T--Exeter--KOpercent2.jpg" | <img src="https://static.igem.org/mediawiki/2016/0/01/T--Exeter--KOpercent2.jpg" | ||
style="max-width:100%;margin:auto;display:block;"> | style="max-width:100%;margin:auto;display:block;"> | ||
− | <span class="caption">Fig. | + | <span class="caption">Fig. 4. Percentage viable cells of KillerOrange exposed to light.</span> |
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Revision as of 12:46, 16 October 2016