Difference between revisions of "Team:Vilnius-Lithuania/Design"
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| − | <h1 class="Raleway" style="text-align:center;"> | + | <h1 class="Raleway" style="text-align:center;">Project</h1> |
<span class="line" style="margin:0 auto;"> </span> | <span class="line" style="margin:0 auto;"> </span> | ||
| − | <p class="Raleway"> | + | <p class="Raleway">Our project has three main approaches which enable more efficient and liable performance of the designed probiotic.</p> |
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| + | <h2 class="Raleway">PAL</h2> | ||
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| + | <p class="Raleway">The first approach is described by the expression of phenylalanine metabolizing enzyme – phenylalanine ammonia lyase (PAL). Originally, human body uses another enzyme called phenylalanine hydroxylase (PAH) to break down this amino acid; however, this enzyme requires oxygen as a co-factor and acts in the bloodstream. Normally, oxygen is not abundant in the intestinal tract, so PAL happens to be more applicable. When breaking down phenylalanine, cinammic acid and ammonia are produced, both of which are considered to be harmless to human body in the occuring concentrations.</p> | ||
| + | |||
| + | <p class="Raleway">For this approach, PAL sequence from already existing biobrick in iGEM parts registry was used (BBa_K1692004). We have slightly changed the sequence by adding His-Tags to C-terminal, N-terminal and to both C- and N-termini instead of the Flag-Tags which originally existed in the biobrick. His-Tags were added with the help of oligonucleotide primers.</p> | ||
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</div> | </div> | ||
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Revision as of 23:13, 16 October 2016
Project
Our project has three main approaches which enable more efficient and liable performance of the designed probiotic.
PAL
The first approach is described by the expression of phenylalanine metabolizing enzyme – phenylalanine ammonia lyase (PAL). Originally, human body uses another enzyme called phenylalanine hydroxylase (PAH) to break down this amino acid; however, this enzyme requires oxygen as a co-factor and acts in the bloodstream. Normally, oxygen is not abundant in the intestinal tract, so PAL happens to be more applicable. When breaking down phenylalanine, cinammic acid and ammonia are produced, both of which are considered to be harmless to human body in the occuring concentrations.
For this approach, PAL sequence from already existing biobrick in iGEM parts registry was used (BBa_K1692004). We have slightly changed the sequence by adding His-Tags to C-terminal, N-terminal and to both C- and N-termini instead of the Flag-Tags which originally existed in the biobrick. His-Tags were added with the help of oligonucleotide primers.
