Safety
Safe Project Design
Every strains used during this competition were no-pathogenic (i.e., Escherichia coli), therefore, no special safety features were used.
The project has been thinking to used transgenic bacterias in a controlled environment and plans to grow them in a bioreactor.
Safe Lab Work
Our trainer, Chantal Socia, and us
Before the beginning of the competition, all of the team members followed a well-being and safety formation with Chantal Socia, a dedicated member of the Laboratoire d'Ingénierie des Systèmes Macromoléculaires [http://lism.cnrs-mrs.fr/ (LISM)].
Every day, each member used a waterless hand cleaner before and after experiments. Benches were disinfected with bleach and one bench was dedicated for BET visualization procedures. Lab coat was used every time, gloves were used for molecular biology (e.g., Plasmid DNA Purification, PCR) and biochemestry (e.g., Electrophoresis), glasses and mask were used for procedure such as prelimary HPLC protocol (High Performance Liquid Chromatography) or gel extraction. Biowastes and chemical wastes were sorted in the respect of the lab rules. Laboratory hood were only used for HPLC derivatization reaction step.
Safe Shipment
No safety problems have been encoutered in sending DNA parts to the Registry.
