Difference between revisions of "Team:Exeter/Parts"
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<h6>Name:</h6> | <h6>Name:</h6> | ||
| − | <p id="pp"> | + | <p id="pp">pT7 Lysozyme C E.coli codon optimised, signal peptide, flag tag</p> |
<h6>Description:</h6> | <h6>Description:</h6> | ||
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<p id="pp">Lysozyme C from chicken egg white, attacks the cell wall in bacteria by hydrolysing the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine of peptidoglycan [4].</p> | <p id="pp">Lysozyme C from chicken egg white, attacks the cell wall in bacteria by hydrolysing the β-1,4 linkages between N-acetylmuramic acid and N-acetylglucosamine of peptidoglycan [4].</p> | ||
| − | <p id="pp"> We are submitting this Lysozyme C as a composite part under a T7 promoter, an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015). We have codon optimised the protein coding region, added a FLAG tag and signal peptide directing Lysozyme to the periplasm. The sequence | + | <p id="pp"> We are submitting this Lysozyme C as a composite part under a T7 promoter, an Elowitz ribosome binding site (BBa_B0034) and a double terminator (BBa_B0015). We have codon optimised the protein coding region, added a FLAG tag and signal peptide directing Lysozyme to the periplasm. The sequence can be found here (BBa_K1914005).</p> |
<p id="pp">Characterisation of this part involved using the Enzcheck assay kit which detects lysozyme activity as a measure of fluorescence as well as using the same protocol we used to characterise KillerRed (<a href="https://2016.igem.org/Team:Exeter/Project#section_4">protocol</a>)and the results of which can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a></p> | <p id="pp">Characterisation of this part involved using the Enzcheck assay kit which detects lysozyme activity as a measure of fluorescence as well as using the same protocol we used to characterise KillerRed (<a href="https://2016.igem.org/Team:Exeter/Project#section_4">protocol</a>)and the results of which can be found <a href="https://2016.igem.org/Team:Exeter/Project#section_2">here</a></p> | ||
Revision as of 20:27, 17 October 2016
