Difference between revisions of "Team:Aix-Marseille/Notebook WIP"

Line 82: Line 82:
 
<ul><li>Transformations DesA, DesB, DesC, DesD, FliC E.Coli and desulfo, interlab studies</li>
 
<ul><li>Transformations DesA, DesB, DesC, DesD, FliC E.Coli and desulfo, interlab studies</li>
 
<li>Transformations failed</li>
 
<li>Transformations failed</li>
−
...
+
<li>Transformation pSB1T3 miniprep, pSB1K3 miniprep, control</li>
 +
</ul>
 +
 
 +
<h5>Tuesday, June 28th<h5>
 +
<ul><li>Miniprep promoter+RBS</li>
 +
<li>PCR of IDT sequences (fliC and DesA to D)
 +
<li>Gel purification K3, C3, T3</li>
 +
</ul>
 +
 
 +
<h5>Wednesday, June 29th</h5>
 +
<ul><li>Miniprep from interlab studies, T3 and K3
 +
<li>Ligation and transformation
 +
<li>Transformation FliC in Tg1
 +
<li>Ligation in C3 transformation in Tg1 DesA to D FliC coli desulfo
 +
</ul>
 +
 
 +
<h5>Thursday, June 30th</h5>
 +
<ul><li>Migration FliC coli, desulfo, DesB, DesC
 +
</ul>
 +
 
 +
<h5>Friday, July 1st</h5>
 +
<ul><li>Digestion PCR products and vector pSB1C3</li>
 +
<li>PCR clean up on digested pSB1C3, fliC coli desulfo, DesB and DesC, migration and transformation in Tg1<li>
 +
</ul>
 +
 
 +
 
 +
<h5>Monday, July 4th</h5>
 +
<ul><li>Isolated white colonies on dishes
 +
</ul>
 +
 
 +
<h5>Tuesday, July 5th</h5>
 +
<ul><li>Reisolated colonies from 04/07
 
</ul>
 
</ul>
  
  
 +
<h5>
  
 
</div>
 
</div>
 
</html>
 
</html>

Revision as of 09:31, 6 July 2016

Monday, June 13th
  • Preparation of heat competent DH5α cells
Tuesday, June 14th
  • Transformation of DH5α cells with test plasmid
Wednesday, June 15th
  • Preparation of heat competent Tg1 cells
  • Transformation of Tg1 cells with test plasmid
Thursday, June 16th
  • Transformation of DH5α cells with pSB1C3 + RFP/li>
  • Transformation with K880005, K823017, I0500, K1404006, B0034
Friday, June 17th
  • Miniprep K880005, K823017, I0500, K1404006, B0034
  • Miniprep pSB1C3 + RFP
Monday, June 20th
  • pSB1C3 digestion from 17/06 miniprep
  • pSB1C3 digestion gel migration : NO PHOTO
  • Biobricks minipreps gel migration : OK PHOTO
  • Transformation Tg1 with RFP+pSB1C3
Tuesday, June 21th
  • pSB1C3 digestion and gel migration, NucleoSpin gel purification and PCR cleanup
  • FliC E.Coli and FliC Desulfo digestion E/P and ligation with pSB1C3
  • E. Coli and Desulfo FliC ligation in linearized pSB1C3
  • Transformation of pSB1C3 RFP in Tg1
Wednesday, June 22th
  • Transformation in DH5α of :
    • FliC coli/desulfo in pSB1C3
    • Linearized pSB1K3
    • Linearized pSB1A3
    • Linearized pSB1T3
    • Linearized pSB1C3
  • Miniprep B0034
  • Transformation in DH5α of interlab studies plasmids
Thursday, June 23th
  • Digestion E/P pSB1C3
  • Purification pSB1C3 purified linearized 23/06
  • Digestion E/P of DesA, DesB, DesC, DesD
  • 3A Assembly of B0034 and I0500 in pSB1K3
  • Digestion with pSB1C3 23/06 insert digested fliC
  • Transformation in Tg1 and DH5α
Friday, June 24th
  • Digestion E/P pSB1C3, pSB1A3, pSB1K3, pSB1T3, and agarose gel purification
  • Colony PCR of
    • RBS+promoter
    • GFP
    • empty
  • Ligation: FliC E.Coli, FliC desulfo, DesA B C D
  • Ligation pSB1C3 and fliC E.Coli / desulfo digested E/P 21/06
  • Transformation in Tg1 and DH5α
  • Redone transformation test device 1 and 3 interlab studies
  • Miniprep pSB1C3 A3 K3 T3
Monday, June 27th
  • Transformations DesA, DesB, DesC, DesD, FliC E.Coli and desulfo, interlab studies
  • Transformations failed
  • Transformation pSB1T3 miniprep, pSB1K3 miniprep, control
Tuesday, June 28th
  • Miniprep promoter+RBS
  • PCR of IDT sequences (fliC and DesA to D)
  • Gel purification K3, C3, T3
Wednesday, June 29th
  • Miniprep from interlab studies, T3 and K3
  • Ligation and transformation
  • Transformation FliC in Tg1
  • Ligation in C3 transformation in Tg1 DesA to D FliC coli desulfo
Thursday, June 30th
  • Migration FliC coli, desulfo, DesB, DesC
Friday, July 1st
  • Digestion PCR products and vector pSB1C3
  • PCR clean up on digested pSB1C3, fliC coli desulfo, DesB and DesC, migration and transformation in Tg1
Monday, July 4th
  • Isolated white colonies on dishes
Tuesday, July 5th
  • Reisolated colonies from 04/07