Difference between revisions of "Team:ASIJ Tokyo/Safety"

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                   <h1>Anderson Promoters </h1>
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                   <h1>Safety</h1>
  
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<h3> Types </h3>
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<h3> Safety Information </h3>
 
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<h4>  
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Anderson Promoters
 
 
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Strength: Weak (0.33) BBa_J23110
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Chassis Organism: <i> E. coli </i>, strain K-12
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<li>Wanted a weak promoter to measure its PET plastic degradation up against the other promoters</li>
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<br>
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Strength: moderate (0.58) BBa_J23111
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In addition to the chassis organism, ASIJ_Tokyo will be working with a synthesized DNA sequence found in Ideonella Sakaiensis called PETase.
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<li>Wanted a moderate promoter to measure its PET plastic degradation up against the other promoters</li>
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Strength: strong (1) BBa_J23100</li>
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Goal
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<li>Wanted a strong promoter to measure its PET plastic degradation up against the other promoters</li>
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Our project is to construct a usable PETase biobrick. This will be accomplished by optimizing the production and secretion of a PET degrading enzyme called PETase. Included within this construction will be an ideal promoter. We will accomplish this within an E. Coli system. We hope that this addition will allow researchers to pursue better methods of of PET degradation in the environment. 
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Risk Level
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E. Coli is a risk group level 1 organism, so there is relatively low risk.  To minimise risk, our group followed safety level 1 procedures. We washed hands after every experiment, disinfected lab benches, and disinfected apparatus. We also worked under biosafety cabinets. To the extent of our knowledge, the bacteria does not pose a risk to human health. Therefore, we believe that the project poses minimal to no risk if developed into a real product.
 
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Revision as of 01:27, 18 October 2016

The BIG TEMPLATE : RESPONSIVE and FREE

Safety

Safety Information


Chassis Organism: E. coli , strain K-12
In addition to the chassis organism, ASIJ_Tokyo will be working with a synthesized DNA sequence found in Ideonella Sakaiensis called PETase.
Goal
Our project is to construct a usable PETase biobrick. This will be accomplished by optimizing the production and secretion of a PET degrading enzyme called PETase. Included within this construction will be an ideal promoter. We will accomplish this within an E. Coli system. We hope that this addition will allow researchers to pursue better methods of of PET degradation in the environment.
Risk Level
E. Coli is a risk group level 1 organism, so there is relatively low risk. To minimise risk, our group followed safety level 1 procedures. We washed hands after every experiment, disinfected lab benches, and disinfected apparatus. We also worked under biosafety cabinets. To the extent of our knowledge, the bacteria does not pose a risk to human health. Therefore, we believe that the project poses minimal to no risk if developed into a real product.